Restriction map for pF1KE0168
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaJI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsoBI       BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrDI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DpnII       DraI        DraII       DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HhaI        HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MboI        MboII       MfeI        
MflI        MluI        MroI        MseI        MspA1I      
MunI        MvnI        NarI        NciI        NcoI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        StuI        StyI        
SwaI        Tru9I       Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI          318  GCGG                 CCGC(-3/-1)
AcyI          213  GGCGTC               G[GA]^CG[CT]C
AflIII          9  ACATGT               A^C[GA][CT]GT
AgeI          359  ACCGGT               A^CCGGT
AspHI         178  GTGCTC               G[AT]GC[AT]^C
AvaII         102  GGACC                G^G[AT]CC
BalI          185  TGGCCA               TGG^CCA
BanI          122  GGCACC               G^G[CT][GA]CC
BfaI           16  CTAG                 C^TAG
BsaHI         213  GGCGTC               G[GA]^CG[CT]C
BsaWI         359  ACCGGT               [AT]^CCGG[AT]
BsiHKAI       178  GTGCTC               G[AT]GC[AT]^C
BsmI          388  GAATGC               GAATGC(1/-1)
Bsp1407I      155  TGTACA               T^GTACA
BspLU11I        9  ACATGT               A^CATGT
BsrGI         155  TGTACA               T^GTACA
DdeI          330  CTCAG                C^T[ACGT]AG
DraIII        127  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          185  TGGCCA               [CT]^GGCC[GA]
Hin1I         213  GGCGTC               G[GA]^CG[CT]C
MaeI           16  CTAG                 C^TAG
MluNI         185  TGGCCA               TGG^CCA
MscI          185  TGGCCA               TGG^CCA
MslI          124  CACCACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          287  GCCGGC               GCC^GGC
NgoMIV        287  GCCGGC               G^CCGGC
PflMI         126  CCACTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         359  ACCGGT               A^CCGGT
PstI          204  CTGCAG               CTGCA^G
SfcI          204  CTGCAG               C^T[GA][CT]AG
SmlI          253  CTCAAG               C^T[CT][GA]AG
SphI          338  GCATGC               GCATG^C
SspBI         155  TGTACA               T^GTACA
TaqI          108  TCGA                 T^CGA
TfiI          136  GATTC                G^A[AT]TC
Tsp509I       394  AATT                 ^AATT
TthHB8I       108  TCGA                 T^CGA
Van91I        126  CCACTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BmyI          178  GTGCTC               G[AGT]GC[ACT]^C
              199  GGGCAC              
BsiYI         126  CCACTATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              353  CCACCTACCGG         
BslI          126  CCACTATGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              353  CCACCTACCGG         
Bsp1286I      178  GTGCTC               G[AGT]GC[ACT]^C
              199  GGGCAC              
BsrFI         287  GCCGGC               [GA]^CCGG[CT]
              359  ACCGGT              
BstF5I         23  CATCC                GGATG(2/0)
              366  CATCC               
Cac8I         287  GCCGGC               GC[ACGT]^[ACGT]GC
              338  GCATGC              
Cfr10I        287  GCCGGC               [GA]^CCGG[CT]
              359  ACCGGT              
Cfr13I        102  GGACC                G^G[ACGT]CC
              290  GGCCC               
FokI           23  CATCC                GGATG(9/13)
              366  CATCC               
HaeIII        186  GGCC                 GG^CC
              290  GGCC                
HapII         288  CCGG                 C^CGG
              360  CCGG                
HgaI          110  GACGC                GACGC(5/10)
              214  GCGTC               
HpaII         288  CCGG                 C^CGG
              360  CCGG                
HphI           48  GGTGA                GGTGA(8/7)
              147  TCACC               
MspI          288  CCGG                 C^CGG
              360  CCGG                
NspI            9  ACATGT               [GA]CATG^[CT]
              338  GCATGC              
PleI           97  GACTC                GAGTC(4/5)
              344  GACTC               
Sau96I        102  GGACC                G^G[ACGT]CC
              290  GGCCC               
SfaNI         326  GATGC                GCATC(5/9)
               22  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AfaI        AluI        BbvI        BssKI       BstNI       
EcoRII      Fnu4HI      HinfI       ItaI        MaeIII      
MnlI        MvaI        MwoI        NlaIII      NlaIV       
RsaI        ScrFI       TseI        Tsp45I      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com