Restriction map for pF1KE1079
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AvaI        AviII       
AvrII       BanII       BbeI        BbrPI       BbvCI       
BcgI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspEI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      CpoI        DraII       
DsaI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoO109I    EcoO65I     FseI        FspI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SwaI        Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         2390  GTCTAC               GT^[AC][GT]AC
AlwNI        2618  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       133  AGCGCT               AGC^GCT
Asp700I      2947  GAAACATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1340  GTGCTC               G[AT]GC[AT]^C
AspI         2046  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1860  GGATCC               G^GATCC
BanI         3031  GGTGCC               G^G[CT][GA]CC
BciVI        3135  GGATAC               GTATCC(6/5)
BglII        2713  AGATCT               A^GATCT
BmyI         1340  GTGCTC               G[AGT]GC[ACT]^C
BpmI         1619  CTCCAG               CTGGAG(16/14)
BsgI         1580  CTGCAC               GTGCAG(16/14)
BsiHKAI      1340  GTGCTC               G[AT]GC[AT]^C
Bsp1286I     1340  GTGCTC               G[AGT]GC[ACT]^C
BspDI        2113  ATCGAT               AT^CGAT
BspHI        2292  TCATGA               T^CATGA
BspMI        1086  GCAGGT               ACCTGC(4/8)
BstAPI       1004  GCATTCGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI          134  GCGC                 GCG^C
ClaI         2113  ATCGAT               AT^CGAT
DraIII        400  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         2550  GAAGAG               CTCTTC(1/4)
Eco47III      133  AGCGCT               AGC^GCT
EcoRI        2296  GAATTC               G^AATTC
EcoRV        2137  GATATC               GAT^ATC
EcoT14I      2402  CCAAGG               C^C[AT][AT]GG
FauI          180  GCGGG                CCCGC(4/6)
HaeII         133  AGCGCT               [GA]GCGC^[CT]
HapII        1734  CCGG                 C^CGG
HhaI          134  GCGC                 GCG^C
HinP1I        134  GCGC                 G^CGC
HindIII       174  AAGCTT               A^AGCTT
HpaII        1734  CCGG                 C^CGG
Ksp632I      2550  GAAGAG               CTCTTC(1/4)
MspI         1734  CCGG                 C^CGG
NdeI         2923  CATATG               CA^TATG
PflFI        2046  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2292  TCATGA               T^CATGA
SexAI          27  ACCTGGT              A^CC[AT]GGT
SfcI          657  CTATAG               C^T[GA][CT]AG
SphI          297  GCATGC               GCATG^C
SspI         1231  AATATT               AAT^ATT
StuI         1998  AGGCCT               AGG^CCT
StyI         2402  CCAAGG               C^C[AT][AT]GG
Tth111I      2046  GACTGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2572  CCATCTTCATTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2947  GAAACATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AvaII        1279  GGACC                G^G[AT]CC
             2406  GGACC               
BalI          681  TGGCCA               TGG^CCA
             1879  TGGCCA              
BseRI        2627  CTCCTC               GAGGAG(10/8)
             3000  CTCCTC              
BsiYI          82  CCTACCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2001  CCTTGTAGAGG         
BslI           82  CCTACCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             2001  CCTTGTAGAGG         
BsmAI        3088  GAGAC                GTCTC(1/5)
              994  GTCTC               
Bsp1407I      138  TGTACA               T^GTACA
             2352  TGTACA              
BsrGI         138  TGTACA               T^GTACA
             2352  TGTACA              
DrdI          194  GACATGTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1805  GACAGCATAGTC        
EaeI          681  TGGCCA               [CT]^GGCC[GA]
             1879  TGGCCA              
EcoNI          82  CCTACCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2001  CCTTGTAGAGG         
EcoT22I      1002  ATGCAT               ATGCA^T
             3041  ATGCAT              
MaeII        2205  ACGT                 A^CGT
             2528  ACGT                
MfeI          154  CAATTG               C^AATTG
              734  CAATTG              
MluNI         681  TGGCCA               TGG^CCA
             1879  TGGCCA              
MscI          681  TGGCCA               TGG^CCA
             1879  TGGCCA              
MunI          154  CAATTG               C^AATTG
              734  CAATTG              
NsiI         1002  ATGCAT               ATGCA^T
             3041  ATGCAT              
SspBI         138  TGTACA               T^GTACA
             2352  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        BbsI        BbvI        BclI        
BfaI        BpuAI       BsaJI       BsmFI       BsmI        
BspLU11I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EcoRII      FbaI        Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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