Restriction map for pF1KE0031
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiWI       BsmBI       BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BssHII      BstBI       BstEII      
BstPI       BstXI       BstYI       Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
FauI        FbaI        FseI        HaeII       HgaI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        MaeII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NgoMIV      NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         1060  GTATAC               GT^[AC][GT]AC
AccII          12  CGCG                 CG^CG
AspHI        1001  GAGCTC               G[AT]GC[AT]^C
AviII        1107  TGCGCA               TGC^GCA
BbsI          943  GAAGAC               GAAGAC(2/6)
BbvCI        1252  GCTGAGG              CCTCAGC(-5/-2)
BcgI          646  CGAGGTCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI         943  GAAGAC               GAAGAC(2/6)
BsaBI        1384  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          948  CTGCAC               GTGCAG(16/14)
BsiEI          38  CGACCG               CG[GA][CT]^CG
BsiHKAI      1001  GAGCTC               G[AT]GC[AT]^C
BsmFI         291  GGGAC                GGGAC(10/14)
Bsp1407I      833  TGTACA               T^GTACA
BsrGI         833  TGTACA               T^GTACA
BssSI         727  CACGAG               CACGAG(-5/-1)
Bst1107I     1060  GTATAC               GTA^TAC
BstUI          12  CGCG                 CG^CG
BstZ17I      1060  GTATAC               GTA^TAC
CfoI         1108  GCGC                 GCG^C
DraII        1352  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI          277  GACAGTTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1356  CCTAGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1352  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       335  CCATGG               C^C[AT][AT]GG
EcoT22I       107  ATGCAT               ATGCA^T
FspI         1107  TGCGCA               TGC^GCA
HhaI         1108  GCGC                 GCG^C
HinP1I       1108  GCGC                 G^CGC
HphI         1381  GGTGA                GGTGA(8/7)
KspI           11  CCGCGG               CCGC^GG
MamI         1384  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI           12  CGCG                 CG^CG
NcoI          335  CCATGG               C^CATGG
NdeI          824  CATATG               CA^TATG
NheI          184  GCTAGC               G^CTAGC
NsiI          107  ATGCAT               ATGCA^T
NspI          343  GCATGT               [GA]CATG^[CT]
PleI         1332  GACTC                GAGTC(4/5)
PpuMI        1352  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          747  CTGCAG               CTGCA^G
PvuII        1111  CAGCTG               CAG^CTG
SacI         1001  GAGCTC               GAGCT^C
SacII          11  CCGCGG               CCGC^GG
SapI          998  GAAGAGC              GCTCTTC(1/4)
SfcI          747  CTGCAG               C^T[GA][CT]AG
SspBI         833  TGTACA               T^GTACA
StyI          335  CCATGG               C^C[AT][AT]GG
Tsp45I        599  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          508  GAATTT               [GA]^AATT[CT]
             1461  GAATTT              
ApoI          508  GAATTT               [GA]^AATT[CT]
             1461  GAATTT              
Asp700I       611  GAATGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1275  GAAGAAGTTC          
BanII         362  GAGCCC               G[GA]GC[CT]^C
             1001  GAGCTC              
BcnI          234  CCGGG                CC^[GC]GG
              288  CCGGG               
BsmAI          85  GAGAC                GTCTC(1/5)
             1046  GAGAC               
BsrDI         741  CATTGC               GCAATG(2/0)
              559  GCAATG              
BsrI          372  ACTGG                ACTGG(1/-1)
             1247  CCAGT               
BstAPI        109  GCATACTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              740  GCATTGCCTGC         
DsaI           11  CCGCGG               C^C[GA][CT]GG
              335  CCATGG              
MaeIII        599  GTGAC                ^GT[ACGT]AC
             1010  GTTAC               
MseI          800  TTAA                 T^TAA
              908  TTAA                
MspA1I         11  CCGCGG               C[AC]G^C[GT]G
             1111  CAGCTG              
NciI          234  CCGGG                CC^[GC]GG
              288  CCGGG               
SmlI          541  CTCAAG               C^T[CT][GA]AG
              993  CTTGAG              
StuI          734  AGGCCT               AGG^CCT
             1298  AGGCCT              
TaqI           56  TCGA                 T^CGA
              201  TCGA                
TfiI          616  GATTC                G^A[AT]TC
             1152  GATTC               
Tru9I         800  TTAA                 T^TAA
              908  TTAA                
TthHB8I        56  TCGA                 T^CGA
              201  TCGA                
XmnI          611  GAATGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1275  GAAGAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BfaI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmI        Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HindIII     HinfI       HpaII       
ItaI        Ksp632I     MaeI        MboI        MboII       
MnlI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TseI        Tsp509I     
TspRI       
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