Restriction map for pF1KB5147
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DraII       DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       FbaI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
HpaII       KasI        KspI        MaeI        MfeI        
MluI        MroI        MslI        MspI        MunI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AciI          137  CCGC                 CCGC(-3/-1)
AflIII       1050  ACGTGT               A^C[GA][CT]GT
AlwNI         289  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI            6  CCTAAGG              CC^T[ACGT]AGG
BanII          89  GAGCCC               G[GA]GC[CT]^C
BbrPI        1049  CACGTG               CAC^GTG
BbsI          924  GAAGAC               GAAGAC(2/6)
BciVI         677  GTATCC               GTATCC(6/5)
BmyI           89  GAGCCC               G[AGT]GC[ACT]^C
BpuAI         924  GAAGAC               GAAGAC(2/6)
BsaAI        1049  CACGTG               [CT]AC^GT[GA]
BsaBI         661  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          353  GAGACC               GGTCTC(1/5)
BsmI           45  GCATTC               GAATGC(1/-1)
Bsp1286I       89  GAGCCC               G[AGT]GC[ACT]^C
BstF5I         51  CATCC                GGATG(2/0)
BstYI         876  GGATCT               [GA]^GATC[CT]
Bsu36I          6  CCTAAGG              CC^T[ACGT]AGG
Cac8I         154  GCTGGC               GC[ACGT]^[ACGT]GC
DraI          624  TTTAAA               TTT^AAA
DraIII         70  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I          6  CCTAAGG              CC^T[ACGT]AGG
EcoRV         637  GATATC               GAT^ATC
EcoT14I       477  CCATGG               C^C[AT][AT]GG
EcoT22I       758  ATGCAT               ATGCA^T
FauI          136  CCCGC                CCCGC(4/6)
FokI           51  CATCC                GGATG(9/13)
HindIII       963  AAGCTT               A^AGCTT
HphI         1024  TCACC                GGTGA(8/7)
MaeIII        340  GTCAC                ^GT[ACGT]AC
MamI          661  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          876  GGATCT               [GA]^GATC[CT]
MseI          625  TTAA                 T^TAA
MwoI          934  GCTAACCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          477  CCATGG               C^CATGG
NsiI          758  ATGCAT               ATGCA^T
NspI          827  GCATGT               [GA]CATG^[CT]
PmaCI        1049  CACGTG               CAC^GTG
PmlI         1049  CACGTG               CAC^GTG
PvuII         333  CAGCTG               CAG^CTG
StyI          477  CCATGG               C^C[AT][AT]GG
Tru9I         625  TTAA                 T^TAA
Tsp45I        340  GTCAC                ^GT[GC]AC
Tsp509I      1059  AATT                 ^AATT
XhoII         876  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

Acc65I        373  GGTACC               G^GTACC
              522  GGTACC              
AccII          23  CGCG                 CG^CG
               38  CGCG                
AfaI          374  GTAC                 GT^AC
              523  GTAC                
AlwI          548  GGATC                GGATC(4/5)
              876  GGATC               
Asp718I       373  GGTACC               G^GTACC
              522  GGTACC              
AvaII         133  GGTCC                G^G[AT]CC
              603  GGACC               
BalI          384  TGGCCA               TGG^CCA
              902  TGGCCA              
BanI          373  GGTACC               G^G[CT][GA]CC
              522  GGTACC              
BbvCI         298  GCTGAGG              CCTCAGC(-5/-2)
              838  GCTGAGG             
BsmBI          25  CGTCTC               CGTCTC(1/5)
              596  GAGACG              
BstUI          23  CGCG                 CG^CG
               38  CGCG                
Cfr13I        133  GGTCC                G^G[ACGT]CC
              603  GGACC               
DsaI           74  CCGTGG               C^C[GA][CT]GG
              477  CCATGG              
EaeI          384  TGGCCA               [CT]^GGCC[GA]
              902  TGGCCA              
EarI          564  CTCTTC               CTCTTC(1/4)
              916  GAAGAG              
HaeIII        385  GGCC                 GG^CC
              903  GGCC                
HgaI           36  GACGC                GACGC(5/10)
               24  GCGTC               
HinfI         261  GAATC                G^A[ACGT]TC
             1033  GAATC               
KpnI          373  GGTACC               GGTAC^C
              522  GGTACC              
Ksp632I       564  CTCTTC               CTCTTC(1/4)
              916  GAAGAG              
MaeII         599  ACGT                 A^CGT
             1050  ACGT                
MluNI         384  TGGCCA               TGG^CCA
              902  TGGCCA              
MscI          384  TGGCCA               TGG^CCA
              902  TGGCCA              
MspA1I        137  CCGCTG               C[AC]G^C[GT]G
              333  CAGCTG              
MvnI           23  CGCG                 CG^CG
               38  CGCG                
RsaI          374  GTAC                 GT^AC
              523  GTAC                
Sau96I        133  GGTCC                G^G[ACGT]CC
              603  GGACC               
SfaNI         427  GATGC                GCATC(5/9)
              757  GATGC               
TaqI          669  TCGA                 T^CGA
              819  TCGA                
TfiI          261  GAATC                G^A[AT]TC
             1033  GAATC               
TthHB8I       669  TCGA                 T^CGA
              819  TCGA                
XcmI          284  CCAACCAGGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              535  CCAGGATTTTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AluI        BbvI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsrI        
BssKI       BstNI       DdeI        DpnII       EcoRII      
Fnu4HI      ItaI        MboI        MboII       MnlI        
MvaI        NdeII       NlaIII      NlaIV       PflMI       
Sau3AI      ScrFI       SfcI        TseI        TspRI       
Van91I      
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