Restriction map for pF1KE0095
##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AvrII       BamHI       BciVI       BclI        BfrI        
BglII       BlnI        BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BsrGI       Bst1107I    
BstAPI      BstBI       BstZ17I     ClaI        CpoI        
DraI        DraIII      EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MfeI        
MroI        MunI        NdeI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SalI        
SbfI        SfiI        SfuI        SpeI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        1747  GACGTC               GACGT^C
AccI         2269  GTAGAC               GT^[AC][GT]AC
AcsI         1691  GAATTC               [GA]^AATT[CT]
AgeI         3634  ACCGGT               A^CCGGT
AhdI         1747  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1452  GTGCAC               G^TGCAC
AocI         2134  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       692  AGCGCT               AGC^GCT
ApaLI        1452  GTGCAC               G^TGCAC
ApoI         1691  GAATTC               [GA]^AATT[CT]
AspEI        1747  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI        1198  GCTGAGG              CCTCAGC(-5/-2)
BfaI         1771  CTAG                 C^TAG
BsaWI        3634  ACCGGT               [AT]^CCGG[AT]
BsmBI        1566  CGTCTC               CGTCTC(1/5)
BspHI        1472  TCATGA               T^CATGA
BsrDI        3173  GCAATG               GCAATG(2/0)
BssHII        501  GCGCGC               G^CGCGC
BssSI         751  CTCGTG               CACGAG(-5/-1)
BstYI        1541  AGATCC               [GA]^GATC[CT]
Bsu36I       2134  CCTGAGG              CC^T[ACGT]AGG
DrdI         2875  GACCTGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1747  GACGTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      692  AGCGCT               AGC^GCT
Eco81I       2134  CCTGAGG              CC^T[ACGT]AGG
EcoNI        3129  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1691  GAATTC               G^AATTC
KspI          150  CCGCGG               CCGC^GG
MaeI         1771  CTAG                 C^TAG
MflI         1541  AGATCC               [GA]^GATC[CT]
MluI         2630  ACGCGT               A^CGCGT
MseI         1538  TTAA                 T^TAA
NcoI         1322  CCATGG               C^CATGG
NheI         1770  GCTAGC               G^CTAGC
NotI          823  GCGGCCGC             GC^GGCCGC
PinAI        3634  ACCGGT               A^CCGGT
PshAI         328  GACTGCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1472  TCATGA               T^CATGA
SacII         150  CCGCGG               CCGC^GG
SmlI         1894  CTCAAG               C^T[CT][GA]AG
SnaBI        1532  TACGTA               TAC^GTA
StuI         3098  AGGCCT               AGG^CCT
Tru9I        1538  TTAA                 T^TAA
Tsp509I      1692  AATT                 ^AATT
XhoII        1541  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

ApaI          592  GGGCCC               GGGCC^C
             3769  GGGCCC              
AviII        1034  TGCGCA               TGC^GCA
             1097  TGCGCA              
BbrPI        2562  CACGTG               CAC^GTG
             2638  CACGTG              
BcgI         1705  CGAGTACACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1163  GCACCAACATCG        
BspLU11I     2555  ACATGT               A^CATGT
             3668  ACATGT              
FspI         1034  TGCGCA               TGC^GCA
             1097  TGCGCA              
PmaCI        2562  CACGTG               CAC^GTG
             2638  CACGTG              
PmlI         2562  CACGTG               CAC^GTG
             2638  CACGTG              
PpuMI        2576  GGGACCT              [GA]G^G[AT]CC[CT]
             2917  GGGACCT             
ScaI         2255  AGTACT               AGT^ACT
             2723  AGTACT              
SexAI        2060  ACCAGGT              A^CC[AT]GGT
             3674  ACCAGGT             
SgrAI          56  CGCCGGCG             C[GA]^CCGG[CT]G
             3633  CACCGGTG            
SphI         1037  GCATGC               GCATG^C
             2937  GCATGC              
TfiI          106  GATTC                G^A[AT]TC
             1521  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvI        BcnI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeII       MaeIII      MamI        MboI        
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com