Restriction map for pF1KE0093
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AocI        AscI        AseI        AsnI        AviII       
AvrII       BalI        BamHI       BbrPI       BciVI       
BfrI        BlnI        BsaBI       BsaWI       BseAI       
BsiWI       BspDI       BspEI       BsrDI       BstBI       
Bsu36I      ClaI        DraI        DrdI        EarI        
Eco81I      EcoRV       EcoT22I     FseI        FspI        
HpaI        Ksp632I     MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NdeI        
NheI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        SapI        ScaI        
SfuI        SnaBI       SpeI        SspI        SwaI        
TfiI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       3215  GGTACC               G^GTACC
AcsI         2324  GAATTC               [GA]^AATT[CT]
AhdI         2758  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3221  AGCGCT               AGC^GCT
ApoI         2324  GAATTC               [GA]^AATT[CT]
Asp700I      2634  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3215  GGTACC               G^GTACC
AspEI        2758  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI         1129  GAAGAC               GAAGAC(2/6)
BbvCI         574  GCTGAGG              CCTCAGC(-5/-2)
BclI          536  TGATCA               T^GATCA
BfaI         1345  CTAG                 C^TAG
BglII        2252  AGATCT               A^GATCT
BpuAI        1129  GAAGAC               GAAGAC(2/6)
BsaAI        2946  TACGTG               [CT]AC^GT[GA]
BsmI         1446  GAATGC               GAATGC(1/-1)
BspHI         779  TCATGA               T^CATGA
BspLU11I     1672  ACATGT               A^CATGT
Bst1107I      790  GTATAC               GTA^TAC
BstEII       2655  GGTGACC              G^GT[ACGT]ACC
BstPI        2655  GGTGACC              G^GT[ACGT]ACC
BstZ17I       790  GTATAC               GTA^TAC
CpoI         2830  CGGACCG              CG^G[AT]CCG
Eam1105I     2758  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3221  AGCGCT               AGC^GCT
EcoO65I      2655  GGTGACC              G^GT[ACGT]ACC
EcoRI        2324  GAATTC               G^AATTC
FbaI          536  TGATCA               T^GATCA
HindIII       847  AAGCTT               A^AGCTT
KpnI         3215  GGTACC               GGTAC^C
KspI          351  CCGCGG               CCGC^GG
MaeI         1345  CTAG                 C^TAG
NcoI          894  CCATGG               C^CATGG
NotI          353  GCGGCCGC             GC^GGCCGC
PshAI        1132  GACAATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          779  TCATGA               T^CATGA
RsrII        2830  CGGACCG              CG^G[AT]CCG
SacI         2185  GAGCTC               GAGCT^C
SacII         351  CCGCGG               CCGC^GG
SalI         2955  GTCGAC               G^TCGAC
SbfI         3236  CCTGCAGG             CCTGCA^GG
SexAI         971  ACCAGGT              A^CC[AT]GGT
SfiI          445  GGCCTGGTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         438  CGCCGGCG             C[GA]^CCGG[CT]G
Sse8387I     3236  CCTGCAGG             CCTGCA^GG
XcmI         1428  CCAGATCCTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2634  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII       1672  ACATGT               A^C[GA][CT]GT
             2688  ACACGT              
AlwI         1431  GATCC                GGATC(4/5)
             3092  GGATC               
AspI          863  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1948  GACAGGGTC           
BlpI          420  GCTCAGC              GC^T[ACGT]AGC
             1842  GCTGAGC             
Bpu1102I      420  GCTCAGC              GC^T[ACGT]AGC
             1842  GCTGAGC             
BsmBI         414  CGTCTC               CGTCTC(1/5)
             2472  CGTCTC              
Bsp1407I     1044  TGTACA               T^GTACA
             2479  TGTACA              
BsrGI        1044  TGTACA               T^GTACA
             2479  TGTACA              
BssHII        216  GCGCGC               G^CGCGC
              234  GCGCGC              
BstXI        2313  CCAAGATTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2501  CCACAACTCTGG        
BstYI        1430  AGATCC               [GA]^GATC[CT]
             2252  AGATCT              
CelII         420  GCTCAGC              GC^T[ACGT]AGC
             1842  GCTGAGC             
EcoNI        1917  CCTCGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3195  CCTCTGCCAGG         
MflI         1430  AGATCC               [GA]^GATC[CT]
             2252  AGATCT              
MseI          824  TTAA                 T^TAA
              830  TTAA                
PflFI         863  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1948  GACAGGGTC           
PpuMI        1225  AGGTCCC              [GA]G^G[AT]CC[CT]
             1952  GGGTCCT             
SmlI         1318  CTCAAG               C^T[CT][GA]AG
             2531  CTCAAG              
SphI         1400  GCATGC               GCATG^C
             2996  GCATGC              
SspBI        1044  TGTACA               T^GTACA
             2479  TGTACA              
Tru9I         824  TTAA                 T^TAA
              830  TTAA                
Tth111I       863  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1948  GACAGGGTC           
XhoII        1430  AGATCC               [GA]^GATC[CT]
             2252  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        Alw44I      AlwNI       ApaI        ApaLI       
AspHI       AvaI        AvaII       BanI        BanII       
BbeI        BbvI        BcgI        BcnI        BglI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        EclXI       Eco52I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KasI        MaeII       MaeIII      
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmaI        StuI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XmaCI       XmaI        
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