Restriction map for pF1KE0093
 (Linear) (Six-base) MAPSORT of: KIEE0093.seq  Check: 9987  from: 1  to: 3285



KIEE0093.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     155     260     468    1345    3285 
   Size:        155     105     208     877    1940

  Fragments arranged by size: 

                1940     877     208     155     105
 
AccI GT'mk_AC

Cuts at:      0     791    2944    2956    3285 
   Size:        791    2153      12     329

  Fragments arranged by size: 

                2153     791     329      12
 
Acc65I G'GTAC_C

Cuts at:      0    3215    3285 
   Size:       3215      70
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265    1677    2100    3285 
   Size:        265    1412     423    1185

  Fragments arranged by size: 

                1412    1185     423     265
 
AfeI AGC'GCT

Cuts at:      0    3223    3285 
   Size:       3223      62
 
AflIII A'CryG_T

Cuts at:      0    1672    2688    3285 
   Size:       1672    1016     597

  Fragments arranged by size: 

                1672    1016     597
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2763    3285 
   Size:       2763     522
 
AleI CACnn'nnGTG

Cuts at:      0     896    1409    3285 
   Size:        896     513    1876

  Fragments arranged by size: 

                1876     896     513
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1357    1427    3285 
   Size:       1357      70    1858

  Fragments arranged by size: 

                1858    1357      70
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2386    2418    3285 
   Size:       2386      32     867

  Fragments arranged by size: 

                2386     867      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     428     816    1337    1434    2174    2423    2525    3285 
   Size:        428     388     521      97     740     249     102     760

  Fragments arranged by size: 

                 760     740     521     428     388     249     102      97
            
 
ApaI G_GGCC'C

Cuts at:      0     176     514    1979    2722    3285 
   Size:        176     338    1465     743     563

  Fragments arranged by size: 

                1465     743     563     338     176
 
ApaLI G'TGCA_C

Cuts at:      0     142    1999    2841    3285 
   Size:        142    1857     842     444

  Fragments arranged by size: 

                1857     842     444     142
 
ApoI r'AATT_y

Cuts at:      0    2324    3285 
   Size:       2324     961
 
AvaI C'yCGr_G

Cuts at:      0      69     169     311     381    1746    1918    2126    2148 
   Size:         69     100     142      70    1365     172     208      22

Cuts at:   2148    2715    2981    3285 
   Size:        567     266     304

  Fragments arranged by size: 

                1365     567     304     266     208     172     142     100
                  70      69      22
 
BanI G'GyrC_C

Cuts at:      0      10     347     433     890    1496    1552    1577    1648 
   Size:         10     337      86     457     606      56      25      71

Cuts at:   1648    1750    3014    3215    3285 
   Size:        102    1264     201      70

  Fragments arranged by size: 

                1264     606     457     337     201     102      86      71
                  70      56      25      10
 
BanII G_rGCy'C

Cuts at:      0     176     514    1642    1689    1849    1979    2189    2552 
   Size:        176     338    1128      47     160     130     210     363

Cuts at:   2552    2614    2722    3285 
   Size:         62     108     563

  Fragments arranged by size: 

                1128     563     363     338     210     176     160     130
                 108      62      47
 
BbeI G_GCGC'C

Cuts at:      0      14     351     437    1652    3018    3285 
   Size:         14     337      86    1215    1366     267

  Fragments arranged by size: 

                1366    1215     337     267      86      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1136    3285 
   Size:       1136    2149
 
BbvCI CC'TCA_GC

Cuts at:      0     575    3285 
   Size:        575    2710
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      63      97    2451    2485    2673    2707    3285 
   Size:         63      34    2354      34     188      34     578

  Fragments arranged by size: 

                2354     578     188      63      34      34      34
 
BclI T'GATC_A

Cuts at:      0     536    3285 
   Size:        536    2749
 
BglI GCCn_nnn'nGGC

Cuts at:      0     180     404     452    1646    2893    3285 
   Size:        180     224      48    1194    1247     392

  Fragments arranged by size: 

                1247    1194     392     224     180      48
 
BglII A'GATC_T

Cuts at:      0    2252    3285 
   Size:       2252    1033
 
BlpI GC'TnA_GC

Cuts at:      0     421    1843    3285 
   Size:        421    1422    1442

  Fragments arranged by size: 

                1442    1422     421
 
Bme1580I G_kGCm'C

Cuts at:      0      76     146     176     514     893    1979    2003    2722 
   Size:         76      70      30     338     379    1086      24     719

Cuts at:   2722    2845    3034    3285 
   Size:        123     189     251

  Fragments arranged by size: 

                1086     719     379     338     251     189     123      76
                  70      30      24
 
BmgBI CAC'GTC

Cuts at:      0     102     141     632    2691    3285 
   Size:        102      39     491    2059     594

  Fragments arranged by size: 

                2059     594     491     102      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2942    3285 
   Size:       2942     343
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2271    2925    3285 
   Size:       2271     654     360

  Fragments arranged by size: 

                2271     654     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     931    1656    1674    1751    3285 
   Size:        931     725      18      77    1534

  Fragments arranged by size: 

                1534     931     725      77      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     575     590    3285 
   Size:        575      15    2695

  Fragments arranged by size: 

                2695     575      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1303    2516    3285 
   Size:       1303    1213     769

  Fragments arranged by size: 

                1303    1213     769
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     579     697    1144    3146    3285 
   Size:        579     118     447    2002     139

  Fragments arranged by size: 

                2002     579     447     139     118
 
BsaAI yAC'GTr

Cuts at:      0    2948    3285 
   Size:       2948     337
 
BsaHI Gr'CG_yC

Cuts at:      0      11     189     348     434     722    1606    1649    2779 
   Size:         11     178     159      86     288     884      43    1130

Cuts at:   2779    3015    3285 
   Size:        236     270

  Fragments arranged by size: 

                1130     884     288     270     236     178     159      86
                  43      11
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     391     421    1235    1265    3285 
   Size:        391      30     814      30    2020

  Fragments arranged by size: 

                2020     814     391      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     952     962    2905    3285 
   Size:        343     609      10    1943     380

  Fragments arranged by size: 

                1943     609     380     343      10
 
BseYI C'CCAG_C

Cuts at:      0     204     562    1170    1804    2423    3285 
   Size:        204     358     608     634     619     862

  Fragments arranged by size: 

                 862     634     619     608     358     204
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     754    1119    1683    2269    2454    2461    2758 
   Size:        232     522     365     564     586     185       7     297

Cuts at:   2758    3010    3285 
   Size:        252     275

  Fragments arranged by size: 

                 586     564     522     365     297     275     252     232
                 185       7
 
BsiEI CG_ry'CG

Cuts at:      0      98     357    1839    2728    3285 
   Size:         98     259    1482     889     557

  Fragments arranged by size: 

                1482     889     557     259      98
 
BsiHKAI G_wGCw'C

Cuts at:      0     116     146     395     695    2003    2189    2800    2845 
   Size:        116      30     249     300    1308     186     611      45

Cuts at:   2845    2953    3285 
   Size:        108     332

  Fragments arranged by size: 

                1308     611     332     300     249     186     116     108
                  45      30
 
BsmI GAATG_Cn'

Cuts at:      0    1452    3285 
   Size:       1452    1833
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     420    2478    3285 
   Size:        420    2058     807

  Fragments arranged by size: 

                2058     807     420
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     116     146     176     395     514     695     893 
   Size:         76      40      30      30     219     119     181     198

Cuts at:    893    1642    1689    1849    1979    2003    2189    2552    2614 
   Size:        749      47     160     130      24     186     363      62

Cuts at:   2614    2722    2800    2845    2953    3034    3285 
   Size:        108      78      45     108      81     251

  Fragments arranged by size: 

                 749     363     251     219     198     186     181     160
                 130     119     108     108      81      78      76      62
                  47      45      40      30      30      24
 
BspHI T'CATG_A

Cuts at:      0     779    3285 
   Size:        779    2506
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     155     260     305     468    1214    1345    1790    2101 
   Size:        155     105      45     163     746     131     445     311

Cuts at:   2101    2668    2968    3244    3285 
   Size:        567     300     276      41

  Fragments arranged by size: 

                 746     567     445     311     300     276     163     155
                 131     105      45      41
 
BsrBI CCG'CTC

Cuts at:      0      27     663     903    3285 
   Size:         27     636     240    2382

  Fragments arranged by size: 

                2382     636     240      27
 
BsrFI r'CCGG_y

Cuts at:      0     255     439    1838    2727    3285 
   Size:        255     184    1399     889     558

  Fragments arranged by size: 

                1399     889     558     255     184
 
BsrGI T'GTAC_A

Cuts at:      0    1044    2479    3285 
   Size:       1044    1435     806

  Fragments arranged by size: 

                1435    1044     806
 
BssHII G'CGCG_C

Cuts at:      0     216     234    3285 
   Size:        216      18    3051

  Fragments arranged by size: 

                3051     216      18
 
BssSI C'ACGA_G

Cuts at:      0    2475    2874    3149    3285 
   Size:       2475     399     275     136

  Fragments arranged by size: 

                2475     399     275     136
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1392    1742    2093    2997    3029    3285 
   Size:       1392     350     351     904      32     256

  Fragments arranged by size: 

                1392     904     351     350     256      32
 
BstEII G'GTnAC_C

Cuts at:      0    2655    3285 
   Size:       2655     630
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2320    2508    3285 
   Size:       2320     188     777

  Fragments arranged by size: 

                2320     777     188
 
BstYI r'GATC_y

Cuts at:      0    1430    2252    3285 
   Size:       1430     822    1033

  Fragments arranged by size: 

                1430    1033     822
 
BstZ17I GTA'TAC

Cuts at:      0     792    3285 
   Size:        792    2493
 
BtgI C'CryG_G

Cuts at:      0     118     351     741     894    1642    1874    2605    2834 
   Size:        118     233     390     153     748     232     731     229

Cuts at:   2834    3018    3285 
   Size:        184     267

  Fragments arranged by size: 

                 748     731     390     267     233     232     229     184
                 153     118
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1143    3285 
   Size:       1143    2142
 
BtsI GCAGTG_nn'

Cuts at:      0    1107    1429    1740    1828    1933    2357    2442    2449 
   Size:       1107     322     311      88     105     424      85       7

Cuts at:   2449    2741    2998    3285 
   Size:        292     257     287

  Fragments arranged by size: 

                1107     424     322     311     292     287     257     105
                  88      85       7
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1195    1230    3285 
   Size:       1195      35    2055

  Fragments arranged by size: 

                2055    1195      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     699    1559    2103    3285 
   Size:        699     860     544    1182

  Fragments arranged by size: 

                1182     860     699     544
 
EaeI y'GGCC_r

Cuts at:      0      95     354     396     716    1943    2725    3285 
   Size:         95     259      42     320    1227     782     560

  Fragments arranged by size: 

                1227     782     560     320     259      95      42
 
EagI C'GGCC_G

Cuts at:      0      95     354    2725    3285 
   Size:         95     259    2371     560

  Fragments arranged by size: 

                2371     560     259      95
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2465    3285 
   Size:       2465     820
 
EcoICRI GAG'CTC

Cuts at:      0    2187    3285 
   Size:       2187    1098
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     931    1656    1674    1677    1751    2100    3285 
   Size:        265     666     725      18       3      74     349    1185

  Fragments arranged by size: 

                1185     725     666     349     265      74      18       3
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1921    3199    3285 
   Size:       1921    1278      86

  Fragments arranged by size: 

                1921    1278      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0     173     510    1226    1953    1976    2719    2970    3285 
   Size:        173     337     716     727      23     743     251     315

  Fragments arranged by size: 

                 743     727     716     337     315     251     173      23
            
 
EcoRI G'AATT_C

Cuts at:      0    2324    3285 
   Size:       2324     961
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2130    3285 
   Size:       2130    1155
 
HaeII r_GCGC'y

Cuts at:      0      14     351     428     437    1652    3018    3225    3285 
   Size:         14     337      77       9    1215    1366     207      60

  Fragments arranged by size: 

                1366    1215     337     207      77      60      14       9
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     906     938    1192    1195    1224    1227    2054    2086 
   Size:        906      32     254       3      29       3     827      32

Cuts at:   2086    2490    2522    2749    2781    3285 
   Size:        404      32     227      32     504

  Fragments arranged by size: 

                 906     827     504     404     254     227      32      32
                  32      32      29       3       3
 
HincII GTy'rAC

Cuts at:      0    1884    2493    2957    3285 
   Size:       1884     609     464     328

  Fragments arranged by size: 

                1884     609     464     328
 
HindIII A'AGCT_T

Cuts at:      0     847    3285 
   Size:        847    2438
 
KasI G'GCGC_C

Cuts at:      0      10     347     433    1648    3014    3285 
   Size:         10     337      86    1215    1366     271

  Fragments arranged by size: 

                1366    1215     337     271      86      10
 
KpnI G_GTAC'C

Cuts at:      0    3219    3285 
   Size:       3219      66
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1053    3285 
   Size:       1053    2232
 
MslI CAynn'nnrTG

Cuts at:      0     784     838     896    1052    1199    1409    3285 
   Size:        784      54      58     156     147     210    1876

  Fragments arranged by size: 

                1876     784     210     156     147      58      54
 
MspA1I CmG'CkG

Cuts at:      0      79     156     285     353    2078    2283    2420    2673 
   Size:         79      77     129      68    1725     205     137     253

Cuts at:   2673    2807    3285 
   Size:        134     478

  Fragments arranged by size: 

                1725     478     253     205     137     134     129      79
                  77      68
 
NaeI GCC'GGC

Cuts at:      0     257     441    2729    3285 
   Size:        257     184    2288     556

  Fragments arranged by size: 

                2288     556     257     184
 
NarI GG'CG_CC

Cuts at:      0      11     348     434    1649    3015    3285 
   Size:         11     337      86    1215    1366     270

  Fragments arranged by size: 

                1366    1215     337     270      86      11
 
NcoI C'CATG_G

Cuts at:      0     894    3285 
   Size:        894    2391
 
NgoMIV G'CCGG_C

Cuts at:      0     255     439    2727    3285 
   Size:        255     184    2288     558

  Fragments arranged by size: 

                2288     558     255     184
 
NotI GC'GGCC_GC

Cuts at:      0     354    3285 
   Size:        354    2931
 
NspI r_CATG'y

Cuts at:      0    1404    1624    1662    1676    1768    3000    3210    3285 
   Size:       1404     220      38      14      92    1232     210      75

  Fragments arranged by size: 

                1404    1232     220     210      92      75      38      14
            
 
PasI CC'CwG_GG

Cuts at:      0     507    1723    3285 
   Size:        507    1216    1562

  Fragments arranged by size: 

                1562    1216     507
 
PciI A'CATG_T

Cuts at:      0    1672    3285 
   Size:       1672    1613
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     520     816    1170    1182    3285 
   Size:        520     296     354      12    2103

  Fragments arranged by size: 

                2103     520     354     296      12
 
PfoI T'CCnGG_A

Cuts at:      0    1613    1811    1955    3285 
   Size:       1613     198     144    1330

  Fragments arranged by size: 

                1613    1330     198     144
 
PpuMI rG'GwC_Cy

Cuts at:      0    1226    1953    3285 
   Size:       1226     727    1332

  Fragments arranged by size: 

                1332    1226     727
 
PshAI GACnn'nnGTC

Cuts at:      0    1136    3285 
   Size:       1136    2149
 
PspOMI G'GGCC_C

Cuts at:      0     172     510    1975    2718    3285 
   Size:        172     338    1465     743     567

  Fragments arranged by size: 

                1465     743     567     338     172
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     775     807    3285 
   Size:        775      32    2478

  Fragments arranged by size: 

                2478     775      32
 
PstI C_TGCA'G

Cuts at:      0    1294    1462    2049    2077    2678    2978    3241    3285 
   Size:       1294     168     587      28     601     300     263      44

  Fragments arranged by size: 

                1294     601     587     300     263     168      44      28
            
 
PvuII CAG'CTG

Cuts at:      0      79     285    2673    2807    3285 
   Size:         79     206    2388     134     478

  Fragments arranged by size: 

                2388     478     206     134      79
 
RsrII CG'GwC_CG

Cuts at:      0    2831    3285 
   Size:       2831     454
 
SacI G_AGCT'C

Cuts at:      0    2189    3285 
   Size:       2189    1096
 
SacII CC_GC'GG

Cuts at:      0     354    3285 
   Size:        354    2931
 
SalI G'TCGA_C

Cuts at:      0    2955    3285 
   Size:       2955     330
 
SbfI CC_TGCA'GG

Cuts at:      0    3241    3285 
   Size:       3241      44
 
SexAI A'CCwGG_T

Cuts at:      0     971    3285 
   Size:        971    2314
 
SfcI C'TryA_G

Cuts at:      0    1290    1407    1458    2045    2073    2674    2974    3057 
   Size:       1290     117      51     587      28     601     300      83

Cuts at:   3057    3237    3285 
   Size:        180      48

  Fragments arranged by size: 

                1290     601     587     300     180     117      83      51
                  48      28
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     452    3285 
   Size:        452    2833
 
SfoI GGC'GCC

Cuts at:      0      12     349     435    1650    3016    3285 
   Size:         12     337      86    1215    1366     269

  Fragments arranged by size: 

                1366    1215     337     269      86      12
 
SgrAI Cr'CCGG_yG

Cuts at:      0     439    3285 
   Size:        439    2846
 
SmaI CCC'GGG

Cuts at:      0      71     171    1748    2128    2717    2983    3285 
   Size:         71     100    1577     380     589     266     302

  Fragments arranged by size: 

                1577     589     380     302     266     100      71
 
SmlI C'TyrA_G

Cuts at:      0    1318    2531    3285 
   Size:       1318    1213     754

  Fragments arranged by size: 

                1318    1213     754
 
SphI G_CATG'C

Cuts at:      0    1404    3000    3285 
   Size:       1404    1596     285

  Fragments arranged by size: 

                1596    1404     285
 
SrfI GCCC'GGGC

Cuts at:      0    2717    3285 
   Size:       2717     568
 
StuI AGG'CCT

Cuts at:      0     642    1862    2896    3285 
   Size:        642    1220    1034     389

  Fragments arranged by size: 

                1220    1034     642     389
 
StyI C'CwwG_G

Cuts at:      0     894    1793    2261    3285 
   Size:        894     899     468    1024

  Fragments arranged by size: 

                1024     899     894     468
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1239    2583    3285 
   Size:       1239    1344     702

  Fragments arranged by size: 

                1344    1239     702
 
TatI w'GTAC_w

Cuts at:      0    1044    2479    3285 
   Size:       1044    1435     806

  Fragments arranged by size: 

                1435    1044     806
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     391     423    1045    1077    1235    1254    1267    1286 
   Size:        391      32     622      32     158      19      13      19

Cuts at:   1286    1807    1839    3196    3228    3285 
   Size:        521      32    1357      32      57

  Fragments arranged by size: 

                1357     622     521     391     158      57      32      32
                  32      32      19      19      13
 
Tth111I GACn'n_nGTC

Cuts at:      0     866    1951    3285 
   Size:        866    1085    1334

  Fragments arranged by size: 

                1334    1085     866
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1435    3285 
   Size:       1435    1850
 
XmaI C'CCGG_G

Cuts at:      0      69     169    1746    2126    2715    2981    3285 
   Size:         69     100    1577     380     589     266     304

  Fragments arranged by size: 

                1577     589     380     304     266     100      69
 
XmnI GAAnn'nnTTC

Cuts at:      0    2638    3285 
   Size:       2638     647
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MmeI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NotI     NspI     PasI     PciI    PflMI
     PfoI    PpuMI    PshAI   PspOMI     PsrI     PstI    PvuII    RsrII
     SacI    SacII     SalI     SbfI    SexAI     SfcI     SfiI     SfoI
    SgrAI     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BciVI    BfrBI     BmtI    BsaBI    BsaWI    BsiWI
    BspEI    BsrDI    BstBI   Bsu36I     ClaI     DraI     DrdI     EarI
    EcoRV     FseI     FspI    FspAI     HpaI     MfeI     MluI     MscI
     NdeI     NheI     NruI     NsiI     PacI     PmeI     PmlI     PpiI
     PsiI    PspXI     PvuI    SanDI     SapI     ScaI     SgfI    SnaBI
     SpeI     SspI     SwaI     XbaI     XhoI     ZraI
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