Restriction map for pF1KE0092
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AccII       AclI        AgeI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        AspI        
BamHI       BbrPI       BcgI        BsaAI       BsaBI       
BsiEI       BsiWI       Bsp1407I    BspDI       BsrGI       
BssHII      Bst1107I    BstBI       BstUI       BstZ17I     
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoT22I     FseI        
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MunI        MvnI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        3365  GACGTC               GACGT^C
AccI         1958  GTCGAC               GT^[AC][GT]AC
AccIII       3038  TCCGGA               T^CCGGA
AflII        3613  CTTAAG               C^TTAAG
AhdI         2803  GACAATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         3560  GGGCCC               GGGCC^C
Asp700I      1648  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2803  GACAATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         4931  GGCGCC               GGCGC^C
BfrI         3613  CTTAAG               C^TTAAG
BglI         4812  GCCCCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2762  AGATCT               A^GATCT
BsaWI        3038  TCCGGA               [AT]^CCGG[AT]
BseAI        3038  TCCGGA               T^CCGGA
BsmBI        1629  GAGACG               CGTCTC(1/5)
BspEI        3038  TCCGGA               T^CCGGA
BssSI        2516  CACGAG               CACGAG(-5/-1)
BstEII       2361  GGTGACC              G^GT[ACGT]ACC
BstPI        2361  GGTGACC              G^GT[ACGT]ACC
Eam1105I     2803  GACAATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      2361  GGTGACC              G^GT[ACGT]ACC
EcoRI        5191  GAATTC               G^AATTC
KasI         4931  GGCGCC               G^GCGCC
MroI         3038  TCCGGA               T^CCGGA
NarI         4931  GGCGCC               GG^CGCC
NcoI          470  CCATGG               C^CATGG
PaeR7I       4942  CTCGAG               C^TCGAG
SacI         2941  GAGCTC               GAGCT^C
SalI         1958  GTCGAC               G^TCGAC
SapI          724  GAAGAGC              GCTCTTC(1/4)
SbfI         2643  CCTGCAGG             CCTGCA^GG
SexAI        1729  ACCTGGT              A^CC[AT]GGT
SfoI         4931  GGCGCC               GGC^GCC
Sse8387I     2643  CCTGCAGG             CCTGCA^GG
XbaI         1554  TCTAGA               T^CTAGA
XhoI         4942  CTCGAG               C^TCGAG
XmnI         1648  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          522  GAATTT               [GA]^AATT[CT]
             5191  GAATTC              
AflIII       4615  ACATGT               A^C[GA][CT]GT
             5032  ACATGT              
ApoI          522  GAATTT               [GA]^AATT[CT]
             5191  GAATTC              
AviII        1691  TGCGCA               TGC^GCA
             2873  TGCGCA              
AvrII        1119  CCTAGG               C^CTAGG
             3604  CCTAGG              
BlnI         1119  CCTAGG               C^CTAGG
             3604  CCTAGG              
BspHI        1622  TCATGA               T^CATGA
             4827  TCATGA              
BspLU11I     4615  ACATGT               A^CATGT
             5032  ACATGT              
BsrBI        4945  GAGCGG               CCGCTC(-3/-3)
             5533  GAGCGG              
DrdI         2654  GACTCCTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2692  GACCTGCTGGTC        
DsaI          470  CCATGG               C^C[GA][CT]GG
             5094  CCGTGG              
EcoRV        4684  GATATC               GAT^ATC
             4896  GATATC              
FspI         1691  TGCGCA               TGC^GCA
             2873  TGCGCA              
MslI          640  CATCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             5383  CACCACAGTG          
NaeI          389  GCCGGC               GCC^GGC
             3032  GCCGGC              
NdeI         2840  CATATG               CA^TATG
             4901  CATATG              
NgoMIV        389  GCCGGC               G^CCGGC
             3032  GCCGGC              
NspI         4615  ACATGT               [GA]CATG^[CT]
             5032  ACATGT              
PpuMI         323  AGGACCC              [GA]G^G[AT]CC[CT]
             2639  GGGTCCT             
RcaI         1622  TCATGA               T^CATGA
             4827  TCATGA              
SmaI         3557  CCCGGG               CCC^GGG
             3589  CCCGGG              
XcmI         1562  CCACTGCTGACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5140  CCAGAAGGAGATTGG     
XmaCI        3557  CCCGGG               C^CCGGG
             3589  CCCGGG              
XmaI         3557  CCCGGG               C^CCGGG
             3589  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

Acc65I      AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        Asp718I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BciVI       BclI        
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KpnI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
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