Restriction map for pF1KE0084
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        AspEI       AvrII       BamHI       
BbrPI       BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BseAI       
BsiWI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       Bst1107I    BstBI       BstXI       
BstZ17I     Bsu36I      ClaI        DraI        DrdI        
Eam1105I    Eco47III    Eco81I      EcoRV       EcoT22I     
FseI        HpaI        MaeI        MamI        MfeI        
MroI        MunI        NheI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        SalI        SapI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SspBI       SspI        SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII         853  GACGTC               GACGT^C
Acc65I       2492  GGTACC               G^GTACC
AccI          835  GTCTAC               GT^[AC][GT]AC
AflIII        377  ACGCGT               A^C[GA][CT]GT
ApaI         2301  GGGCCC               GGGCC^C
Asp700I      1509  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2492  GGTACC               G^GTACC
AspHI        1129  GAGCTC               G[AT]GC[AT]^C
AspI         2113  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         704  TGCGCA               TGC^GCA
BbeI          135  GGCGCC               GGCGC^C
BbvCI        2207  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2269  CGAGTGAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          932  TGATCA               T^GATCA
BsaWI         722  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      1129  GAGCTC               G[AT]GC[AT]^C
BsmI         1768  GCATTC               GAATGC(1/-1)
BsrBI         118  GAGCGG               CCGCTC(-3/-3)
BsrDI         656  GCAATG               GCAATG(2/0)
BssHII         13  GCGCGC               G^CGCGC
BstAPI        696  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI         2312  CGGACCG              CG^G[AT]CCG
DraIII       2098  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         2255  CTCTTC               CTCTTC(1/4)
EcoNI        2496  CCTCGCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1568  GAATTC               G^AATTC
FbaI          932  TGATCA               T^GATCA
FspI          704  TGCGCA               TGC^GCA
HincII       2383  GTCAAC               GT[CT]^[GA]AC
HindII       2383  GTCAAC               GT[CT]^[GA]AC
HindIII       742  AAGCTT               A^AGCTT
KasI          135  GGCGCC               G^GCGCC
KpnI         2492  GGTACC               GGTAC^C
Ksp632I      2255  CTCTTC               CTCTTC(1/4)
KspI          352  CCGCGG               CCGC^GG
MluI          377  ACGCGT               A^CGCGT
MslI          606  CATCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          106  GCCGGC               GCC^GGC
NarI          135  GGCGCC               GG^CGCC
NcoI         1484  CCATGG               C^CATGG
NdeI         1808  CATATG               CA^TATG
NgoMIV        106  GCCGGC               G^CCGGC
NotI          215  GCGGCCGC             GC^GGCCGC
PflFI        2113  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsrII        2312  CGGACCG              CG^G[AT]CCG
SacI         1129  GAGCTC               GAGCT^C
SacII         352  CCGCGG               CCGC^GG
SbfI         2295  CCTGCAGG             CCTGCA^GG
SfoI          135  GGCGCC               GGC^GCC
SphI          707  GCATGC               GCATG^C
Sse8387I     2295  CCTGCAGG             CCTGCA^GG
StuI         1615  AGGCCT               AGG^CCT
Tth111I      2113  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1259  CCAGCCAAAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1509  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI         1568  GAATTC               [GA]^AATT[CT]
             1597  AAATTC              
AlwNI         697  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1121  CAGCTGCTG           
ApoI         1568  GAATTC               [GA]^AATT[CT]
             1597  AAATTC              
BsgI         1075  GTGCAG               GTGCAG(16/14)
             1954  GTGCAG              
BsiEI         104  CGGCCG               CG[GA][CT]^CG
              216  CGGCCG              
BsrFI         106  GCCGGC               [GA]^CCGG[CT]
              311  ACCGGC              
BstEII       1477  GGTGACC              G^GT[ACGT]ACC
             2103  GGTGACC             
BstPI        1477  GGTGACC              G^GT[ACGT]ACC
             2103  GGTGACC             
BstYI         521  GGATCT               [GA]^GATC[CT]
              698  AGATCC              
Cfr10I        106  GCCGGC               [GA]^CCGG[CT]
              311  ACCGGC              
EagI          104  CGGCCG               C^GGCCG
              216  CGGCCG              
EclXI         104  CGGCCG               C^GGCCG
              216  CGGCCG              
Eco52I        104  CGGCCG               C^GGCCG
              216  CGGCCG              
EcoO65I      1477  GGTGACC              G^GT[ACGT]ACC
             2103  GGTGACC             
MflI          521  GGATCT               [GA]^GATC[CT]
              698  AGATCC              
MseI         1012  TTAA                 T^TAA
             1396  TTAA                
PleI         2253  GACTC                GAGTC(4/5)
             2263  GACTC               
PstI          694  CTGCAG               CTGCA^G
             2296  CTGCAG              
SmlI            7  CTCAAG               C^T[CT][GA]AG
             1069  CTCAAG              
Tru9I        1012  TTAA                 T^TAA
             1396  TTAA                
XhoII         521  GGATCT               [GA]^GATC[CT]
              698  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstUI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      
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