Restriction map for pF1KE0073
##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AviII       AvrII       BbsI        BclI        BfrI        
BglII       BlnI        BpuAI       BsaBI       BsiWI       
Bsp1407I    BspDI       BsrBI       BsrDI       BsrGI       
Bst1107I    BstBI       BstZ17I     ClaI        DraI        
DrdI        EarI        EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshBI       Psp1406I    PvuI        
SacI        SacII       SapI        SexAI       SfuI        
SgrAI       SnaBI       SspBI       SspI        SwaI        
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          682  GTCGAC               GT^[AC][GT]AC
AccIII       2895  TCCGGA               T^CCGGA
AcsI         1113  AAATTC               [GA]^AATT[CT]
AflIII        734  ACATGT               A^C[GA][CT]GT
Alw44I        262  GTGCAC               G^TGCAC
AocI         3566  CCTCAGG              CC^T[ACGT]AGG
ApaLI         262  GTGCAC               G^TGCAC
ApoI         1113  AAATTC               [GA]^AATT[CT]
AspI         2825  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         144  GGATCC               G^GATCC
BciVI        1224  GTATCC               GTATCC(6/5)
BlpI         1726  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1726  GCTCAGC              GC^T[ACGT]AGC
BseAI        2895  TCCGGA               T^CCGGA
BsmBI        2977  GAGACG               CGTCTC(1/5)
BsmI         3912  GCATTC               GAATGC(1/-1)
BspEI        2895  TCCGGA               T^CCGGA
BspHI        1667  TCATGA               T^CATGA
BspLU11I      734  ACATGT               A^CATGT
BstEII       3231  GGTGACC              G^GT[ACGT]ACC
BstPI        3231  GGTGACC              G^GT[ACGT]ACC
Bsu36I       3566  CCTCAGG              CC^T[ACGT]AGG
CelII        1726  GCTCAGC              GC^T[ACGT]AGC
CpoI         3282  CGGACCG              CG^G[AT]CCG
DraIII        550  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       3566  CCTCAGG              CC^T[ACGT]AGG
EcoNI        3393  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3231  GGTGACC              G^GT[ACGT]ACC
MboII        2550  GAAGA                GAAGA(8/7)
MroI         2895  TCCGGA               T^CCGGA
PaeR7I       3881  CTCGAG               C^TCGAG
PflFI        2825  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2845  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1667  TCATGA               T^CATGA
RsrII        3282  CGGACCG              CG^G[AT]CCG
SalI          682  GTCGAC               G^TCGAC
SbfI         1176  CCTGCAGG             CCTGCA^GG
ScaI         2882  AGTACT               AGT^ACT
SfiI         1753  GGCCAGGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         3881  CTCGAG               C^T[CT][GA]AG
SpeI         3204  ACTAGT               A^CTAGT
Sse8387I     1176  CCTGCAGG             CCTGCA^GG
Tth111I      2825  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3881  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI         2883  GTAC                 GT^AC
             3681  GTAC                
AhdI         1552  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3169  GACGAGGTGTC         
Aor51HI      2167  AGCGCT               AGC^GCT
             3670  AGCGCT              
AspEI        1552  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3169  GACGAGGTGTC         
BalI           48  TGGCCA               TGG^CCA
             1358  TGGCCA              
BbrPI        2071  CACGTG               CAC^GTG
             2854  CACGTG              
BbvCI        1042  CCTCAGC              CCTCAGC(-5/-2)
             3806  CCTCAGC             
BsaAI        2071  CACGTG               [CT]AC^GT[GA]
             2854  CACGTG              
BseRI        1295  CTCCTC               GAGGAG(10/8)
             1997  CTCCTC              
BspMI        1922  ACCTGC               ACCTGC(4/8)
             3399  GCAGGT              
BssSI        3150  CACGAG               CACGAG(-5/-1)
              375  CTCGTG              
EagI         2253  CGGCCG               C^GGCCG
             2817  CGGCCG              
Eam1105I     1552  GACCACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3169  GACGAGGTGTC         
EclXI        2253  CGGCCG               C^GGCCG
             2817  CGGCCG              
Eco47III     2167  AGCGCT               AGC^GCT
             3670  AGCGCT              
Eco52I       2253  CGGCCG               C^GGCCG
             2817  CGGCCG              
HincII         31  GTCAAC               GT[CT]^[GA]AC
              682  GTCGAC              
HindII         31  GTCAAC               GT[CT]^[GA]AC
              682  GTCGAC              
MluNI          48  TGGCCA               TGG^CCA
             1358  TGGCCA              
MscI           48  TGGCCA               TGG^CCA
             1358  TGGCCA              
MslI          186  CATGAACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1350  CATGAACGTG          
PmaCI        2071  CACGTG               CAC^GTG
             2854  CACGTG              
PmlI         2071  CACGTG               CAC^GTG
             2854  CACGTG              
PpuMI        2664  AGGACCC              [GA]G^G[AT]CC[CT]
             3076  GGGTCCC             
RsaI         2883  GTAC                 GT^AC
             3681  GTAC                
XcmI         1229  CCATCCACCGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2759  CCAACTACACCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbvI        BcgI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaHI       BsaI        BsaJI       BsaWI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssHII      BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
MaeI        MaeII       MaeIII      MboI        MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmaCI       
XmaI        
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