Restriction map for pF1KE0070
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        Aor51HI     ApaLI       ApoI        AscI        
Asp700I     Asp718I     AspEI       AspI        BamHI       
BbrPI       BcgI        BciVI       BfrI        BglII       
BsaAI       BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BsrDI       BsrGI       Bst1107I    BstYI       BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FseI        HindIII     
KpnI        MamI        MfeI        MflI        MluI        
MroI        MslI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RsrII       SalI        
ScaI        SexAI       SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AfaI          951  GTAC                 GT^AC
AocI          306  CCTGAGG              CC^T[ACGT]AGG
AseI         1656  ATTAAT               AT^TAAT
AsnI         1656  ATTAAT               AT^TAAT
AvrII        1317  CCTAGG               C^CTAGG
BalI         1638  TGGCCA               TGG^CCA
BbsI           82  GTCTTC               GAAGAC(2/6)
BbvCI           3  GCTGAGG              CCTCAGC(-5/-2)
BlnI         1317  CCTAGG               C^CTAGG
BpuAI          82  GTCTTC               GAAGAC(2/6)
BsaI         1198  GAGACC               GGTCTC(1/5)
BseRI        1458  CTCCTC               GAGGAG(10/8)
BsgI         1036  CTGCAC               GTGCAG(16/14)
BsiEI         714  CGACCG               CG[GA][CT]^CG
BspHI         236  TCATGA               T^CATGA
BspLU11I      419  ACATGT               A^CATGT
BsrBI        1381  CCGCTC               CCGCTC(-3/-3)
BssSI        1324  CTCGTG               CACGAG(-5/-1)
BstAPI        159  GCACATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1649  TTCGAA               TT^CGAA
BstEII       1527  GGTGACC              G^GT[ACGT]ACC
BstPI        1527  GGTGACC              G^GT[ACGT]ACC
BstXI        1005  CCAGGGCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        306  CCTGAGG              CC^T[ACGT]AGG
EaeI         1638  TGGCCA               [CT]^GGCC[GA]
Eco81I        306  CCTGAGG              CC^T[ACGT]AGG
EcoNI         552  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1527  GGTGACC              G^GT[ACGT]ACC
HpaI          436  GTTAAC               GTT^AAC
MluNI        1638  TGGCCA               TGG^CCA
MscI         1638  TGGCCA               TGG^CCA
NaeI         1372  GCCGGC               GCC^GGC
NgoMIV       1372  GCCGGC               G^CCGGC
NspI          419  ACATGT               [GA]CATG^[CT]
NspV         1649  TTCGAA               TT^CGAA
PaeR7I        586  CTCGAG               C^TCGAG
PpuMI        1244  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1239  GACGGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1656  ATTAAT               AT^TAAT
RcaI          236  TCATGA               T^CATGA
RsaI          951  GTAC                 GT^AC
SfiI         1420  GGCCCCCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1649  TTCGAA               TT^CGAA
SmlI          586  CTCGAG               C^T[CT][GA]AG
XhoI          586  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AflIII        419  ACATGT               A^C[GA][CT]GT
             1214  ACGTGT              
AvaII         387  GGACC                G^G[AT]CC
             1245  GGTCC               
AviII         905  TGCGCA               TGC^GCA
             1351  TGCGCA              
BbeI          652  GGCGCC               GGCGC^C
              997  GGCGCC              
BclI         1601  TGATCA               T^GATCA
             1610  TGATCA              
BfaI          136  CTAG                 C^TAG
             1318  CTAG                
BglI         1322  GCCTCGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1421  GCCCCCGGGGC         
BlpI          580  GCTAAGC              GC^T[ACGT]AGC
              978  GCTGAGC             
Bpu1102I      580  GCTAAGC              GC^T[ACGT]AGC
              978  GCTGAGC             
BspMI         440  ACCTGC               ACCTGC(4/8)
              551  ACCTGC              
BsrI          432  ACTGG                ACTGG(1/-1)
              488  CCAGT               
BstF5I        162  CATCC                GGATG(2/0)
              687  CATCC               
CelII         580  GCTAAGC              GC^T[ACGT]AGC
              978  GCTGAGC             
FbaI         1601  TGATCA               T^GATCA
             1610  TGATCA              
FokI          162  CATCC                GGATG(9/13)
              687  CATCC               
FspI          905  TGCGCA               TGC^GCA
             1351  TGCGCA              
HincII        436  GTTAAC               GT[CT]^[GA]AC
              810  GTTGAC              
HindII        436  GTTAAC               GT[CT]^[GA]AC
              810  GTTGAC              
KasI          652  GGCGCC               G^GCGCC
              997  GGCGCC              
KspI          183  CCGCGG               CCGC^GG
              973  CCGCGG              
MaeI          136  CTAG                 C^TAG
             1318  CTAG                
MaeII         116  ACGT                 A^CGT
             1214  ACGT                
NarI          652  GGCGCC               GG^CGCC
              997  GGCGCC              
PvuII         901  CAGCTG               CAG^CTG
             1192  CAGCTG              
SacII         183  CCGCGG               CCGC^GG
              973  CCGCGG              
SbfI          441  CCTGCAGG             CCTGCA^GG
              873  CCTGCAGG            
SfaNI         125  GCATC                GCATC(5/9)
              333  GCATC               
SfoI          652  GGCGCC               GGC^GCC
              997  GGCGCC              
Sse8387I      441  CCTGCAGG             CCTGCA^GG
              873  CCTGCAGG            
StuI          138  AGGCCT               AGG^CCT
             1320  AGGCCT              
Tsp509I      1635  AATT                 ^AATT
             1655  AATT                
TspRI         100  CAGTG                CA[GC]TG[ACGT][ACGT]^
              282  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwNI       
ApaI        AspHI       AvaI        BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BssHII      
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MseI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfcI        SmaI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      TthHB8I     XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com