Restriction map for pF1KE0070
 (Linear) (Six-base) MAPSORT of: KIEE0070.seq  Check: 1280  from: 1  to: 1680



KIEE0070.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     560    1680 
   Size:        560    1120
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     264     535    1680 
   Size:        192      72     271    1145

  Fragments arranged by size: 

                1145     271     192      72
 
AflIII A'CryG_T

Cuts at:      0     419    1214    1680 
   Size:        419     795     466

  Fragments arranged by size: 

                 795     466     419
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     919    1138    1680 
   Size:        919     219     542

  Fragments arranged by size: 

                 919     542     219
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     655     687    1680 
   Size:        655      32     993

  Fragments arranged by size: 

                 993     655      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     432     882    1360    1680 
   Size:        432     450     478     320

  Fragments arranged by size: 

                 478     450     432     320
 
ApaI G_GGCC'C

Cuts at:      0    1292    1392    1423    1432    1521    1680 
   Size:       1292     100      31       9      89     159

  Fragments arranged by size: 

                1292     159     100      89      31       9
 
AseI AT'TA_AT

Cuts at:      0    1657    1680 
   Size:       1657      23
 
AvaI C'yCGr_G

Cuts at:      0      31     105     586     703     843    1416    1424    1680 
   Size:         31      74     481     117     140     573       8     256

  Fragments arranged by size: 

                 573     481     256     140     117      74      31       8
            
 
AvrII C'CTAG_G

Cuts at:      0    1317    1680 
   Size:       1317     363
 
BanI G'GyrC_C

Cuts at:      0     274     652     997    1680 
   Size:        274     378     345     683

  Fragments arranged by size: 

                 683     378     345     274
 
BanII G_rGCy'C

Cuts at:      0     452     842     973    1022    1227    1292    1343    1392 
   Size:        452     390     131      49     205      65      51      49

Cuts at:   1392    1423    1432    1521    1674    1680 
   Size:         31       9      89     153       6

  Fragments arranged by size: 

                 452     390     205     153     131      89      65      51
                  49      49      31       9       6
 
BbeI G_GCGC'C

Cuts at:      0     656    1001    1680 
   Size:        656     345     679

  Fragments arranged by size: 

                 679     656     345
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      75    1680 
   Size:         75    1605
 
BbvCI CC'TCA_GC

Cuts at:      0       4    1680 
   Size:          4    1676
 
BclI T'GATC_A

Cuts at:      0    1601    1610    1680 
   Size:       1601       9      70

  Fragments arranged by size: 

                1601      70       9
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1328    1427    1680 
   Size:       1328      99     253

  Fragments arranged by size: 

                1328     253      99
 
BlpI GC'TnA_GC

Cuts at:      0     581     979    1680 
   Size:        581     398     701

  Fragments arranged by size: 

                 701     581     398
 
Bme1580I G_kGCm'C

Cuts at:      0    1143    1259    1292    1392    1417    1423    1432    1521 
   Size:       1143     116      33     100      25       6       9      89

Cuts at:   1521    1680 
   Size:        159

  Fragments arranged by size: 

                1143     159     116     100      89      33      25       9
                   6
 
BmgBI CAC'GTC

Cuts at:      0     117    1215    1680 
   Size:        117    1098     465

  Fragments arranged by size: 

                1098     465     117
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      29    1680 
   Size:         29    1651
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     526    1006    1186    1216    1680 
   Size:        391     135     480     180      30     464

  Fragments arranged by size: 

                 480     464     391     180     135      30
 
Bpu10I CC'TnA_GC

Cuts at:      0       4     400     937    1680 
   Size:          4     396     537     743

  Fragments arranged by size: 

                 743     537     396       4
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1192    1680 
   Size:       1192     488
 
BsaHI Gr'CG_yC

Cuts at:      0      92     188     653     998    1680 
   Size:         92      96     465     345     682

  Fragments arranged by size: 

                 682     465     345      96      92
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     364     394     584     614     655     685    1234    1264 
   Size:        364      30     190      30      41      30     549      30

Cuts at:   1264    1680 
   Size:        416

  Fragments arranged by size: 

                 549     416     364     190      41      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1449    1680 
   Size:       1449     231
 
BseYI C'CCAG_C

Cuts at:      0      47     899    1479    1520    1680 
   Size:         47     852     580      41     160

  Fragments arranged by size: 

                 852     580     160      47      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1021    1680 
   Size:       1021     659
 
BsiEI CG_ry'CG

Cuts at:      0     717    1680 
   Size:        717     963
 
BsiHKAI G_wGCw'C

Cuts at:      0      32     106     368     452     576     842     854    1022 
   Size:         32      74     262      84     124     266      12     168

Cuts at:   1022    1031    1680 
   Size:          9     649

  Fragments arranged by size: 

                 649     266     262     168     124      84      74      32
                  12       9
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     106     368     452     576     842     854     973 
   Size:         32      74     262      84     124     266      12     119

Cuts at:    973    1022    1031    1143    1227    1259    1292    1343    1392 
   Size:         49       9     112      84      32      33      51      49

Cuts at:   1392    1417    1423    1432    1521    1674    1680 
   Size:         25       6       9      89     153       6

  Fragments arranged by size: 

                 266     262     153     124     119     112      89      84
                  84      74      51      49      49      33      32      32
                  25      12       9       9       6       6
 
BspHI T'CATG_A

Cuts at:      0     236    1680 
   Size:        236    1444
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     449     560    1680 
   Size:        449     111    1120

  Fragments arranged by size: 

                1120     449     111
 
BsrBI CCG'CTC

Cuts at:      0    1383    1680 
   Size:       1383     297
 
BsrFI r'CCGG_y

Cuts at:      0     716    1372    1524    1680 
   Size:        716     656     152     156

  Fragments arranged by size: 

                 716     656     156     152
 
BssHII G'CGCG_C

Cuts at:      0      42     121     983    1125    1680 
   Size:         42      79     862     142     555

  Fragments arranged by size: 

                 862     555     142      79      42
 
BssSI C'ACGA_G

Cuts at:      0    1324    1680 
   Size:       1324     356
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     165    1680 
   Size:        165    1515
 
BstBI TT'CG_AA

Cuts at:      0    1650    1680 
   Size:       1650      30
 
BstEII G'GTnAC_C

Cuts at:      0    1527    1680 
   Size:       1527     153
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1012    1680 
   Size:       1012     668
 
Bsu36I CC'TnA_GG

Cuts at:      0     307    1680 
   Size:        307    1373
 
BtgI C'CryG_G

Cuts at:      0     183     973    1345    1532    1680 
   Size:        183     790     372     187     148

  Fragments arranged by size: 

                 790     372     187     183     148
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     681    1680 
   Size:        681     999
 
BtsI GCAGTG_nn'

Cuts at:      0     106    1680 
   Size:        106    1574
 
EaeI y'GGCC_r

Cuts at:      0    1638    1680 
   Size:       1638      42
 
EarI CTCTTCn'nnn_

Cuts at:      0     175     499     859    1150    1331    1680 
   Size:        175     324     360     291     181     349

  Fragments arranged by size: 

                 360     349     324     291     181     175
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      53     249    1680 
   Size:         53     196    1431

  Fragments arranged by size: 

                1431     196      53
 
EcoICRI GAG'CTC

Cuts at:      0     450     840    1020    1680 
   Size:        450     390     180     660

  Fragments arranged by size: 

                 660     450     390     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     264     391     526     535    1006    1186    1216 
   Size:        192      72     127     135       9     471     180      30

Cuts at:   1216    1680 
   Size:        464

  Fragments arranged by size: 

                 471     464     192     180     135     127      72      30
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     556    1680 
   Size:        556    1124
 
EcoO109I rG'GnC_Cy

Cuts at:      0     707     870    1008    1245    1288    1289    1357    1389 
   Size:        707     163     138     237      43       1      68      32

Cuts at:   1389    1420    1428    1680 
   Size:         31       8     252

  Fragments arranged by size: 

                 707     252     237     163     138      68      43      32
                  31       8       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      99    1680 
   Size:         99    1581
 
FspI TGC'GCA

Cuts at:      0     907    1353    1680 
   Size:        907     446     327

  Fragments arranged by size: 

                 907     446     327
 
HaeII r_GCGC'y

Cuts at:      0     234     656    1001    1272    1680 
   Size:        234     422     345     271     408

  Fragments arranged by size: 

                 422     408     345     271     234
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     584     616    1444    1476    1680 
   Size:        584      32     828      32     204

  Fragments arranged by size: 

                 828     584     204      32      32
 
HincII GTy'rAC

Cuts at:      0     438     812    1680 
   Size:        438     374     868

  Fragments arranged by size: 

                 868     438     374
 
HpaI GTT'AAC

Cuts at:      0     438    1680 
   Size:        438    1242
 
KasI G'GCGC_C

Cuts at:      0     652     997    1680 
   Size:        652     345     683

  Fragments arranged by size: 

                 683     652     345
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     269     503    1067    1680 
   Size:        269     234     564     613

  Fragments arranged by size: 

                 613     564     269     234
 
MscI TGG'CCA

Cuts at:      0    1640    1680 
   Size:       1640      40
 
MspA1I CmG'CkG

Cuts at:      0     185     212     831     903     975    1194    1447    1680 
   Size:        185      27     619      72      72     219     253     233

  Fragments arranged by size: 

                 619     253     233     219     185      72      72      27
            
 
NaeI GCC'GGC

Cuts at:      0    1374    1680 
   Size:       1374     306
 
NarI GG'CG_CC

Cuts at:      0     653     998    1680 
   Size:        653     345     682

  Fragments arranged by size: 

                 682     653     345
 
NgoMIV G'CCGG_C

Cuts at:      0    1372    1680 
   Size:       1372     308
 
NspI r_CATG'y

Cuts at:      0     423    1680 
   Size:        423    1257
 
PasI CC'CwG_GG

Cuts at:      0    1360    1508    1680 
   Size:       1360     148     172

  Fragments arranged by size: 

                1360     172     148
 
PciI A'CATG_T

Cuts at:      0     419    1680 
   Size:        419    1261
 
PfoI T'CCnGG_A

Cuts at:      0      86     368    1680 
   Size:         86     282    1312

  Fragments arranged by size: 

                1312     282      86
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     655     687    1680 
   Size:        655      32     993

  Fragments arranged by size: 

                 993     655      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1245    1680 
   Size:       1245     435
 
PshAI GACnn'nnGTC

Cuts at:      0    1243    1680 
   Size:       1243     437
 
PspOMI G'GGCC_C

Cuts at:      0    1288    1388    1419    1428    1517    1680 
   Size:       1288     100      31       9      89     163

  Fragments arranged by size: 

                1288     163     100      89      31       9
 
PstI C_TGCA'G

Cuts at:      0     197     230     446     671     878    1680 
   Size:        197      33     216     225     207     802

  Fragments arranged by size: 

                 802     225     216     207     197      33
 
PvuII CAG'CTG

Cuts at:      0     903    1194    1680 
   Size:        903     291     486

  Fragments arranged by size: 

                 903     486     291
 
SacI G_AGCT'C

Cuts at:      0     452     842    1022    1680 
   Size:        452     390     180     658

  Fragments arranged by size: 

                 658     452     390     180
 
SacII CC_GC'GG

Cuts at:      0     186     976    1680 
   Size:        186     790     704

  Fragments arranged by size: 

                 790     704     186
 
SapI GCTCTTCn'nnn_

Cuts at:      0     175     859    1331    1680 
   Size:        175     684     472     349

  Fragments arranged by size: 

                 684     472     349     175
 
SbfI CC_TGCA'GG

Cuts at:      0     446     878    1680 
   Size:        446     432     802

  Fragments arranged by size: 

                 802     446     432
 
SfcI C'TryA_G

Cuts at:      0     193     226     442     667     874    1680 
   Size:        193      33     216     225     207     806

  Fragments arranged by size: 

                 806     225     216     207     193      33
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1427    1680 
   Size:       1427     253
 
SfoI GGC'GCC

Cuts at:      0     654     999    1680 
   Size:        654     345     681

  Fragments arranged by size: 

                 681     654     345
 
SmaI CCC'GGG

Cuts at:      0     705     845    1418    1426    1680 
   Size:        705     140     573       8     254

  Fragments arranged by size: 

                 705     573     254     140       8
 
SmlI C'TyrA_G

Cuts at:      0     586    1680 
   Size:        586    1094
 
SrfI GCCC'GGGC

Cuts at:      0    1418    1680 
   Size:       1418     262
 
StuI AGG'CCT

Cuts at:      0     140    1322    1680 
   Size:        140    1182     358

  Fragments arranged by size: 

                1182     358     140
 
StyI C'CwwG_G

Cuts at:      0     557    1011    1317    1484    1674    1680 
   Size:        557     454     306     167     190       6

  Fragments arranged by size: 

                 557     454     306     190     167       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     913    1493    1500    1680 
   Size:        913     580       7     180

  Fragments arranged by size: 

                 913     580     180       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     321     353     676     708    1680 
   Size:        321      32     323      32     972

  Fragments arranged by size: 

                 972     323     321      32      32
 
XhoI C'TCGA_G

Cuts at:      0     586    1680 
   Size:        586    1094
 
XmaI C'CCGG_G

Cuts at:      0     703     843    1416    1424    1680 
   Size:        703     140     573       8     256

  Fragments arranged by size: 

                 703     573     256     140       8
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI     AloI    AlwNI     ApaI     AseI
     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI     BclI
     BglI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I     BsaI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII
    Hin4I   HincII     HpaI     KasI     MmeI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PasI     PciI     PfoI     PpiI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SacI    SacII     SapI     SbfI
     SfcI     SfiI     SfoI     SmaI     SmlI     SrfI     StuI     StyI
    TaqII     TstI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI    ApaLI     ApoI     AscI    AsiSI     BaeI    BamHI     BcgI
    BciVI    BfrBI    BglII     BmrI     BmtI    BpuEI    BsaAI    BsaBI
    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI    BstYI
  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoRI
    EcoRV     FseI    FspAI  HindIII     KpnI     MfeI     MluI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI
     PmeI     PmlI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
    SanDI     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SspI     SwaI     TatI  Tth111I     XbaI     XcmI     XmnI     ZraI
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