Restriction map for pF1KE0061
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AhdI        AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BbsI        BbvCI       BcgI        BglI        
BglII       BlnI        BpuAI       BsaBI       BsiWI       
BspDI       BspHI       BspLU11I    BstAPI      BstBI       
ClaI        CpoI        DraIII      DrdI        Eam1105I    
EarI        EcoT14I     EcoT22I     FseI        FspI        
HindIII     HpaI        Ksp632I     MamI        MfeI        
MunI        NcoI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspI        
StuI        StyI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I       1096  GGTACC               G^GTACC
AccIII        615  TCCGGA               T^CCGGA
AclI          760  AACGTT               AA^CGTT
AflII         891  CTTAAG               C^TTAAG
AgeI         1836  ACCGGT               A^CCGGT
AlwNI         148  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2329  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1231  AGCGCT               AGC^GCT
ApaI         2286  GGGCCC               GGGCC^C
Asp700I       414  GAATCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1096  GGTACC               G^GTACC
BalI         2476  TGGCCA               TGG^CCA
BbeI         1698  GGCGCC               GGCGC^C
BfrI          891  CTTAAG               C^TTAAG
BlpI         2168  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2168  GCTCAGC              GC^T[ACGT]AGC
BseAI         615  TCCGGA               T^CCGGA
BsmBI        1915  GAGACG               CGTCTC(1/5)
BsmI         2438  GCATTC               GAATGC(1/-1)
BspEI         615  TCCGGA               T^CCGGA
BsrBI        1539  GAGCGG               CCGCTC(-3/-3)
BsrDI        2055  CATTGC               GCAATG(2/0)
Bsu36I       2329  CCTCAGG              CC^T[ACGT]AGG
CelII        2168  GCTCAGC              GC^T[ACGT]AGC
DraI         2566  TTTAAA               TTT^AAA
EagI         1844  CGGCCG               C^GGCCG
EclXI        1844  CGGCCG               C^GGCCG
Eco47III     1231  AGCGCT               AGC^GCT
Eco52I       1844  CGGCCG               C^GGCCG
Eco81I       2329  CCTCAGG              CC^T[ACGT]AGG
EcoRI         258  GAATTC               G^AATTC
EcoRV         481  GATATC               GAT^ATC
KasI         1698  GGCGCC               G^GCGCC
KpnI         1096  GGTACC               GGTAC^C
KspI         1744  CCGCGG               CCGC^GG
MluI         1811  ACGCGT               A^CGCGT
MluNI        2476  TGGCCA               TGG^CCA
MroI          615  TCCGGA               T^CCGGA
MscI         2476  TGGCCA               TGG^CCA
NaeI         1686  GCCGGC               GCC^GGC
NarI         1698  GGCGCC               GG^CGCC
NdeI          919  CATATG               CA^TATG
NgoMIV       1686  GCCGGC               G^CCGGC
NheI         1315  GCTAGC               G^CTAGC
PflMI        1764  CCATGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1836  ACCGGT               A^CCGGT
PpuMI         149  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I      760  AACGTT               AA^CGTT
PvuII        2556  CAGCTG               CAG^CTG
SacII        1744  CCGCGG               CCGC^GG
SexAI        1092  ACCAGGT              A^CC[AT]GGT
SfoI         1698  GGCGCC               GGC^GCC
SmlI          891  CTTAAG               C^T[CT][GA]AG
SphI         2405  GCATGC               GCATG^C
Van91I       1764  CCATGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          872  CCAAGTTCCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          414  GAATCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          738  GTATAC               GT^[AC][GT]AC
             1377  GTATAC              
Alw44I       1528  GTGCAC               G^TGCAC
             2419  GTGCAC              
ApaLI        1528  GTGCAC               G^TGCAC
             2419  GTGCAC              
BciVI          16  GGATAC               GTATCC(6/5)
             2485  GTATCC              
BclI         2099  TGATCA               T^GATCA
             2466  TGATCA              
BsaAI        1519  TACGTG               [CT]AC^GT[GA]
             1781  TACGTG              
BsaI         2068  GAGACC               GGTCTC(1/5)
             2722  GAGACC              
BsgI          303  GTGCAG               GTGCAG(16/14)
             1784  GTGCAG              
BsiEI         270  CGACCG               CG[GA][CT]^CG
             1844  CGGCCG              
BssSI        1573  CACGAG               CACGAG(-5/-1)
             1901  CACGAG              
Bst1107I      738  GTATAC               GTA^TAC
             1377  GTATAC              
BstEII       1152  GGTTACC              G^GT[ACGT]ACC
             1272  GGTTACC             
BstPI        1152  GGTTACC              G^GT[ACGT]ACC
             1272  GGTTACC             
BstZ17I       738  GTATAC               GTA^TAC
             1377  GTATAC              
EcoNI        2325  CCTTCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2424  CCTAGAGGAGG         
EcoO65I      1152  GGTTACC              G^GT[ACGT]ACC
             1272  GGTTACC             
FbaI         2099  TGATCA               T^GATCA
             2466  TGATCA              
MslI          787  CACCCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2211  CACTCTGGTG          
NspI         2191  GCATGT               [GA]CATG^[CT]
             2405  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaHI       
BsaJI       BsaWI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspMI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspBI       
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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