Restriction map for pF1KE0061
 (Linear) (Six-base) MAPSORT of: KIEE0061.seq  Check: 2873  from: 1  to: 2811



KIEE0061.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1644    2811 
   Size:       1644    1167
 
AccI GT'mk_AC

Cuts at:      0     739    1378    2811 
   Size:        739     639    1433

  Fragments arranged by size: 

                1433     739     639
 
Acc65I G'GTAC_C

Cuts at:      0    1096    2811 
   Size:       1096    1715
 
AclI AA'CG_TT

Cuts at:      0     761    2811 
   Size:        761    2050
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2298    2811 
   Size:       2298     513
 
AfeI AGC'GCT

Cuts at:      0    1233    2811 
   Size:       1233    1578
 
AflII C'TTAA_G

Cuts at:      0     891    2811 
   Size:        891    1920
 
AflIII A'CryG_T

Cuts at:      0    1520    1811    2090    2811 
   Size:       1520     291     279     721

  Fragments arranged by size: 

                1520     721     291     279
 
AgeI A'CCGG_T

Cuts at:      0    1836    2811 
   Size:       1836     975
 
AleI CACnn'nnGTG

Cuts at:      0    2215    2811 
   Size:       2215     596
 
AlwNI CAG_nnn'CTG

Cuts at:      0     153    2811 
   Size:        153    2658
 
ApaI G_GGCC'C

Cuts at:      0    2290    2811 
   Size:       2290     521
 
ApaLI G'TGCA_C

Cuts at:      0    1528    2419    2811 
   Size:       1528     891     392

  Fragments arranged by size: 

                1528     891     392
 
ApoI r'AATT_y

Cuts at:      0     205     258    2620    2811 
   Size:        205      53    2362     191

  Fragments arranged by size: 

                2362     205     191      53
 
AvaI C'yCGr_G

Cuts at:      0     106     658    1372    1703    2811 
   Size:        106     552     714     331    1108

  Fragments arranged by size: 

                1108     714     552     331     106
 
BanI G'GyrC_C

Cuts at:      0     177     670    1096    1698    2022    2811 
   Size:        177     493     426     602     324     789

  Fragments arranged by size: 

                 789     602     493     426     324     177
 
BanII G_rGCy'C

Cuts at:      0     520    1294    1908    1970    2290    2811 
   Size:        520     774     614      62     320     521

  Fragments arranged by size: 

                 774     614     521     520     320      62
 
BbeI G_GCGC'C

Cuts at:      0    1702    2811 
   Size:       1702    1109
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      10    2496    2811 
   Size:         10    2486     315

  Fragments arranged by size: 

                2486     315      10
 
BclI T'GATC_A

Cuts at:      0    2099    2466    2811 
   Size:       2099     367     345

  Fragments arranged by size: 

                2099     367     345
 
BlpI GC'TnA_GC

Cuts at:      0    2169    2811 
   Size:       2169     642
 
Bme1580I G_kGCm'C

Cuts at:      0      10    1532    2290    2423    2811 
   Size:         10    1522     758     133     388

  Fragments arranged by size: 

                1522     758     388     133      10
 
BmgBI CAC'GTC

Cuts at:      0     360     543    1341    2811 
   Size:        360     183     798    1470

  Fragments arranged by size: 

                1470     798     360     183
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     712     949    2811 
   Size:        712     237    1862

  Fragments arranged by size: 

                1862     712     237
 
BmtI G_CTAG'C

Cuts at:      0    1319    2811 
   Size:       1319    1492
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     106    2811 
   Size:        106    2705
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     244     337     828     842    1603    2208    2513 
   Size:        181      63      93     491      14     761     605     305

Cuts at:   2513    2811 
   Size:        298

  Fragments arranged by size: 

                 761     605     491     305     298     181      93      63
                  14
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2062    2716    2811 
   Size:       2062     654      95

  Fragments arranged by size: 

                2062     654      95
 
BsaAI yAC'GTr

Cuts at:      0    1521    1783    2811 
   Size:       1521     262    1028

  Fragments arranged by size: 

                1521    1028     262
 
BsaHI Gr'CG_yC

Cuts at:      0    1085    1168    1699    2032    2811 
   Size:       1085      83     531     333     779

  Fragments arranged by size: 

                1085     779     531     333      83
 
BsaWI w'CCGG_w

Cuts at:      0     390     615    1836    2596    2811 
   Size:        390     225    1221     760     215

  Fragments arranged by size: 

                1221     760     390     225     215
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     826     856    2374    2404    2811 
   Size:        826      30    1518      30     407

  Fragments arranged by size: 

                1518     826     407      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     292    1591    2443    2734    2811 
   Size:        124     168    1299     852     291      77

  Fragments arranged by size: 

                1299     852     291     168     124      77
 
BseYI C'CCAG_C

Cuts at:      0     399    2252    2320    2811 
   Size:        399    1853      68     491

  Fragments arranged by size: 

                1853     491     399      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     324    1805    2811 
   Size:        324    1481    1006

  Fragments arranged by size: 

                1481    1006     324
 
BsiEI CG_ry'CG

Cuts at:      0     273    1847    2811 
   Size:        273    1574     964

  Fragments arranged by size: 

                1574     964     273
 
BsiHKAI G_wGCw'C

Cuts at:      0     945    1532    2170    2224    2278    2423    2811 
   Size:        945     587     638      54      54     145     388

  Fragments arranged by size: 

                 945     638     587     388     145      54      54
 
BsmI GAATG_Cn'

Cuts at:      0    2438    2811 
   Size:       2438     373
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1909    2811 
   Size:       1909     902
 
Bsp1286I G_dGCh'C

Cuts at:      0      10     520     945    1294    1532    1908    1970    2170 
   Size:         10     510     425     349     238     376      62     200

Cuts at:   2170    2224    2278    2290    2423    2811 
   Size:         54      54      12     133     388

  Fragments arranged by size: 

                 510     425     388     376     349     238     200     133
                  62      54      54      12      10
 
BspEI T'CCGG_A

Cuts at:      0     615    2811 
   Size:        615    2196
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     333     552    1165    1644    2811 
   Size:        333     219     613     479    1167

  Fragments arranged by size: 

                1167     613     479     333     219
 
BsrBI CCG'CTC

Cuts at:      0    1541    2811 
   Size:       1541    1270
 
BsrDI GCAATG_nn'

Cuts at:      0    2054    2811 
   Size:       2054     757
 
BsrFI r'CCGG_y

Cuts at:      0    1686    1836    1846    2811 
   Size:       1686     150      10     965

  Fragments arranged by size: 

                1686     965     150      10
 
BsrGI T'GTAC_A

Cuts at:      0    1000    1877    2150    2811 
   Size:       1000     877     273     661

  Fragments arranged by size: 

                1000     877     661     273
 
BssHII G'CGCG_C

Cuts at:      0    1503    1505    1607    1609    2811 
   Size:       1503       2     102       2    1202

  Fragments arranged by size: 

                1503    1202     102       2       2
 
BssSI C'ACGA_G

Cuts at:      0    1573    1901    2811 
   Size:       1573     328     910

  Fragments arranged by size: 

                1573     910     328
 
BstEII G'GTnAC_C

Cuts at:      0    1152    1272    2811 
   Size:       1152     120    1539

  Fragments arranged by size: 

                1539    1152     120
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1995    2130    2232    2811 
   Size:       1995     135     102     579

  Fragments arranged by size: 

                1995     579     135     102
 
BstYI r'GATC_y

Cuts at:      0     216     950    2635    2811 
   Size:        216     734    1685     176

  Fragments arranged by size: 

                1685     734     216     176
 
BstZ17I GTA'TAC

Cuts at:      0     740    1379    2811 
   Size:        740     639    1432

  Fragments arranged by size: 

                1432     740     639
 
Bsu36I CC'TnA_GG

Cuts at:      0    2330    2811 
   Size:       2330     481
 
BtgI C'CryG_G

Cuts at:      0     210    1325    1744    1959    2811 
   Size:        210    1115     419     215     852

  Fragments arranged by size: 

                1115     852     419     215     210
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     126     408    1628    2088    2811 
   Size:        126     282    1220     460     723

  Fragments arranged by size: 

                1220     723     460     282     126
 
BtsI GCAGTG_nn'

Cuts at:      0    1310    1690    1733    2479    2811 
   Size:       1310     380      43     746     332

  Fragments arranged by size: 

                1310     746     380     332      43
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     192     227    2811 
   Size:        192      35    2584

  Fragments arranged by size: 

                2584     192      35
 
DraI TTT'AAA

Cuts at:      0    2568    2811 
   Size:       2568     243
 
EaeI y'GGCC_r

Cuts at:      0     638    1334    1844    2016    2476    2811 
   Size:        638     696     510     172     460     335

  Fragments arranged by size: 

                 696     638     510     460     335     172
 
EagI C'GGCC_G

Cuts at:      0    1844    2811 
   Size:       1844     967
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     184     306    1245    2811 
   Size:        184     122     939    1566

  Fragments arranged by size: 

                1566     939     184     122
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     244     337     828     842    1603    2208    2298 
   Size:        181      63      93     491      14     761     605      90

Cuts at:   2298    2513    2811 
   Size:        215     298

  Fragments arranged by size: 

                 761     605     491     298     215     181      93      90
                  63      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2329    2428    2811 
   Size:       2329      99     383

  Fragments arranged by size: 

                2329     383      99
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150    2286    2461    2811 
   Size:        150    2136     175     350

  Fragments arranged by size: 

                2136     350     175     150
 
EcoRI G'AATT_C

Cuts at:      0     258    2811 
   Size:        258    2553
 
EcoRV GAT'ATC

Cuts at:      0     483    2811 
   Size:        483    2328
 
HaeII r_GCGC'y

Cuts at:      0    1235    1702    2176    2811 
   Size:       1235     467     474     635

  Fragments arranged by size: 

                1235     635     474     467
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     454     486     853     885    1237    1269    1276    1308 
   Size:        454      32     367      32     352      32       7      32

Cuts at:   1308    1783    1815    2811 
   Size:        475      32     996

  Fragments arranged by size: 

                 996     475     454     367     352      32      32      32
                  32      32       7
 
HincII GTy'rAC

Cuts at:      0    1049    1143    1791    2088    2547    2811 
   Size:       1049      94     648     297     459     264

  Fragments arranged by size: 

                1049     648     459     297     264      94
 
KasI G'GCGC_C

Cuts at:      0    1698    2811 
   Size:       1698    1113
 
KpnI G_GTAC'C

Cuts at:      0    1100    2811 
   Size:       1100    1711
 
MluI A'CGCG_T

Cuts at:      0    1811    2811 
   Size:       1811    1000
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1547    2811 
   Size:       1547    1264
 
MscI TGG'CCA

Cuts at:      0    2478    2811 
   Size:       2478     333
 
MslI CAynn'nnrTG

Cuts at:      0     791    2215    2811 
   Size:        791    1424     596

  Fragments arranged by size: 

                1424     791     596
 
MspA1I CmG'CkG

Cuts at:      0     339    1746    2558    2811 
   Size:        339    1407     812     253

  Fragments arranged by size: 

                1407     812     339     253
 
NaeI GCC'GGC

Cuts at:      0    1688    2811 
   Size:       1688    1123
 
NarI GG'CG_CC

Cuts at:      0    1699    2811 
   Size:       1699    1112
 
NdeI CA'TA_TG

Cuts at:      0     920    2811 
   Size:        920    1891
 
NgoMIV G'CCGG_C

Cuts at:      0    1686    2811 
   Size:       1686    1125
 
NheI G'CTAG_C

Cuts at:      0    1315    2811 
   Size:       1315    1496
 
NspI r_CATG'y

Cuts at:      0    2195    2409    2811 
   Size:       2195     214     402

  Fragments arranged by size: 

                2195     402     214
 
PasI CC'CwG_GG

Cuts at:      0      25    2811 
   Size:         25    2786
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1770    2811 
   Size:       1770    1041
 
PfoI T'CCnGG_A

Cuts at:      0     536    2454    2811 
   Size:        536    1918     357

  Fragments arranged by size: 

                1918     536     357
 
PpuMI rG'GwC_Cy

Cuts at:      0     150    2811 
   Size:        150    2661
 
PsiI TTA'TAA

Cuts at:      0      55    2811 
   Size:         55    2756
 
PspOMI G'GGCC_C

Cuts at:      0    2286    2811 
   Size:       2286     525
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     723     755    1369    1401    2811 
   Size:        723      32     614      32    1410

  Fragments arranged by size: 

                1410     723     614      32      32
 
PstI C_TGCA'G

Cuts at:      0     239     344    1562    1655    2811 
   Size:        239     105    1218      93    1156

  Fragments arranged by size: 

                1218    1156     239     105      93
 
PvuII CAG'CTG

Cuts at:      0    2558    2811 
   Size:       2558     253
 
SacII CC_GC'GG

Cuts at:      0    1747    2811 
   Size:       1747    1064
 
SexAI A'CCwGG_T

Cuts at:      0    1092    2811 
   Size:       1092    1719
 
SfcI C'TryA_G

Cuts at:      0     235     340    1558    1651    2345    2811 
   Size:        235     105    1218      93     694     466

  Fragments arranged by size: 

                1218     694     466     235     105      93
 
SfoI GGC'GCC

Cuts at:      0    1700    2811 
   Size:       1700    1111
 
SmlI C'TyrA_G

Cuts at:      0     891    2811 
   Size:        891    1920
 
SphI G_CATG'C

Cuts at:      0    2409    2811 
   Size:       2409     402
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      90    1142    1699    2086    2307    2811 
   Size:         90    1052     557     387     221     504

  Fragments arranged by size: 

                1052     557     504     387     221      90
 
TatI w'GTAC_w

Cuts at:      0    1000    1877    2150    2811 
   Size:       1000     877     273     661

  Fragments arranged by size: 

                1000     877     661     273
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     764     796     935     967    2811 
   Size:        764      32     139      32    1844

  Fragments arranged by size: 

                1844     764     139      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     879    2811 
   Size:        879    1932
 
XmnI GAAnn'nnTTC

Cuts at:      0     418    2811 
   Size:        418    2393
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI    BciVI     BclI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI     EaeI     EagI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NspI     PasI    PflMI     PfoI    PpuMI     PsiI   PspOMI
     PsrI     PstI    PvuII    SacII    SexAI     SfcI     SfoI     SmlI
     SphI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AhdI     AlfI     AloI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BcgI    BfrBI     BglI    BglII
   Bpu10I    BpuEI    BsaBI    BsiWI    BspHI   BstAPI    BstBI     ClaI
   DraIII     DrdI     EarI  EcoICRI     FalI     FseI     FspI    FspAI
  HindIII     HpaI     MfeI     NcoI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PpiI    PshAI    PspXI     PvuI    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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