Restriction map for pF1KE0060
 (Linear) (Six-base) MAPSORT of: KIEE0060.seq  Check: 5298  from: 1  to: 1725



KIEE0060.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1668    1725 
   Size:       1668      57
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1217    1574    1725 
   Size:       1217     357     151

  Fragments arranged by size: 

                1217     357     151
 
AflIII A'CryG_T

Cuts at:      0     666     689    1725 
   Size:        666      23    1036

  Fragments arranged by size: 

                1036     666      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0      83    1047    1725 
   Size:         83     964     678

  Fragments arranged by size: 

                 964     678      83
 
ApaI G_GGCC'C

Cuts at:      0     153    1725 
   Size:        153    1572
 
ApoI r'AATT_y

Cuts at:      0       8     562    1725 
   Size:          8     554    1163

  Fragments arranged by size: 

                1163     554       8
 
AvaI C'yCGr_G

Cuts at:      0     146     777    1725 
   Size:        146     631     948

  Fragments arranged by size: 

                 948     631     146
 
AvrII C'CTAG_G

Cuts at:      0    1063    1725 
   Size:       1063     662
 
BanII G_rGCy'C

Cuts at:      0     153     987    1725 
   Size:        153     834     738

  Fragments arranged by size: 

                 834     738     153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     310     738    1547    1725 
   Size:        310     428     809     178

  Fragments arranged by size: 

                 809     428     310     178
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1372    1725 
   Size:       1372     353
 
BglII A'GATC_T

Cuts at:      0    1122    1725 
   Size:       1122     603
 
Bme1580I G_kGCm'C

Cuts at:      0     153    1725 
   Size:        153    1572
 
BmgBI CAC'GTC

Cuts at:      0     669    1725 
   Size:        669    1056
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     529    1725 
   Size:        529    1196
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1094    1407    1653    1725 
   Size:       1094     313     246      72

  Fragments arranged by size: 

                1094     313     246      72
 
Bpu10I CC'TnA_GC

Cuts at:      0    1094    1725 
   Size:       1094     631
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     556    1725 
   Size:        201     355    1169

  Fragments arranged by size: 

                1169     355     201
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     847    1725 
   Size:        847     878
 
BsaBI GATnn'nnATC

Cuts at:      0    1276    1725 
   Size:       1276     449
 
BsaWI w'CCGG_w

Cuts at:      0      89     438    1702    1725 
   Size:         89     349    1264      23

  Fragments arranged by size: 

                1264     349      89      23
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     794     824    1090    1120    1405    1435    1500    1530 
   Size:        794      30     266      30     285      30      65      30

Cuts at:   1530    1725 
   Size:        195

  Fragments arranged by size: 

                 794     285     266     195      65      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     328     815     905    1725 
   Size:        328     487      90     820

  Fragments arranged by size: 

                 820     487     328      90
 
BseYI C'CCAG_C

Cuts at:      0      54    1225    1254    1725 
   Size:         54    1171      29     471

  Fragments arranged by size: 

                1171     471      54      29
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     615    1725 
   Size:        615    1110
 
Bsp1286I G_dGCh'C

Cuts at:      0     153     987    1725 
   Size:        153     834     738

  Fragments arranged by size: 

                 834     738     153
 
BspEI T'CCGG_A

Cuts at:      0      89     438    1725 
   Size:         89     349    1287

  Fragments arranged by size: 

                1287     349      89
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1668    1725 
   Size:       1668      57
 
BsrBI CCG'CTC

Cuts at:      0     131     252    1725 
   Size:        131     121    1473

  Fragments arranged by size: 

                1473     131     121
 
BsrDI GCAATG_nn'

Cuts at:      0    1190    1455    1683    1725 
   Size:       1190     265     228      42

  Fragments arranged by size: 

                1190     265     228      42
 
BsrFI r'CCGG_y

Cuts at:      0     237    1510    1725 
   Size:        237    1273     215

  Fragments arranged by size: 

                1273     237     215
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1192    1725 
   Size:       1192     533
 
BstEII G'GTnAC_C

Cuts at:      0     396    1725 
   Size:        396    1329
 
BstYI r'GATC_y

Cuts at:      0    1077    1122    1725 
   Size:       1077      45     603

  Fragments arranged by size: 

                1077     603      45
 
BtgI C'CryG_G

Cuts at:      0     806    1372    1457    1725 
   Size:        806     566      85     268

  Fragments arranged by size: 

                 806     566     268      85
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     396    1725 
   Size:        396    1329
 
DraI TTT'AAA

Cuts at:      0     560    1725 
   Size:        560    1165
 
DraIII CAC_nnn'GTG

Cuts at:      0     471    1725 
   Size:        471    1254
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     115     376    1725 
   Size:        115     261    1349

  Fragments arranged by size: 

                1349     261     115
 
EaeI y'GGCC_r

Cuts at:      0    1460    1725 
   Size:       1460     265
 
EarI CTCTTCn'nnn_

Cuts at:      0      21    1389    1593    1609    1725 
   Size:         21    1368     204      16     116

  Fragments arranged by size: 

                1368     204     116      21      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1094    1217    1407    1574    1653    1725 
   Size:       1094     123     190     167      79      72

  Fragments arranged by size: 

                1094     190     167     123      79      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0      80     369    1640    1725 
   Size:         80     289    1271      85

  Fragments arranged by size: 

                1271     289      85      80
 
EcoRI G'AATT_C

Cuts at:      0       8    1725 
   Size:          8    1717
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1638    1725 
   Size:       1638      87
 
HaeII r_GCGC'y

Cuts at:      0     217    1725 
   Size:        217    1508
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     465     497     511     543     794     826    1725 
   Size:        465      32      14      32     251      32     899

  Fragments arranged by size: 

                 899     465     251      32      32      32      14
 
HincII GTy'rAC

Cuts at:      0     644    1322    1725 
   Size:        644     678     403

  Fragments arranged by size: 

                 678     644     403
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      78     200     215    1725 
   Size:         78     122      15    1510

  Fragments arranged by size: 

                1510     122      78      15
 
MscI TGG'CCA

Cuts at:      0    1462    1725 
   Size:       1462     263
 
MspA1I CmG'CkG

Cuts at:      0    1374    1673    1725 
   Size:       1374     299      52

  Fragments arranged by size: 

                1374     299      52
 
NcoI C'CATG_G

Cuts at:      0     806    1457    1725 
   Size:        806     651     268

  Fragments arranged by size: 

                 806     651     268
 
NspI r_CATG'y

Cuts at:      0     693    1725 
   Size:        693    1032
 
PciI A'CATG_T

Cuts at:      0     689    1725 
   Size:        689    1036
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     240    1725 
   Size:        240    1485
 
PfoI T'CCnGG_A

Cuts at:      0    1071    1725 
   Size:       1071     654
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     356     388    1725 
   Size:        356      32    1337

  Fragments arranged by size: 

                1337     356      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      80     369    1725 
   Size:         80     289    1356

  Fragments arranged by size: 

                1356     289      80
 
PsiI TTA'TAA

Cuts at:      0     959    1725 
   Size:        959     766
 
PspOMI G'GGCC_C

Cuts at:      0     149    1725 
   Size:        149    1576
 
PstI C_TGCA'G

Cuts at:      0    1052    1122    1725 
   Size:       1052      70     603

  Fragments arranged by size: 

                1052     603      70
 
SacII CC_GC'GG

Cuts at:      0    1375    1725 
   Size:       1375     350
 
SfcI C'TryA_G

Cuts at:      0     342    1048    1118    1206    1443    1725 
   Size:        342     706      70      88     237     282

  Fragments arranged by size: 

                 706     342     282     237      88      70
 
SmaI CCC'GGG

Cuts at:      0     148    1725 
   Size:        148    1577
 
SmlI C'TyrA_G

Cuts at:      0     180     571    1725 
   Size:        180     391    1154

  Fragments arranged by size: 

                1154     391     180
 
StyI C'CwwG_G

Cuts at:      0     610     806    1063    1457    1644    1725 
   Size:        610     196     257     394     187      81

  Fragments arranged by size: 

                 610     394     257     196     187      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      63     384    1459    1521    1725 
   Size:         63     321    1075      62     204

  Fragments arranged by size: 

                1075     321     204      63      62
 
TatI w'GTAC_w

Cuts at:      0     906    1725 
   Size:        906     819
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1500    1532    1725 
   Size:       1500      32     193

  Fragments arranged by size: 

                1500     193      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     394     535    1725 
   Size:        394     141    1190

  Fragments arranged by size: 

                1190     394     141
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     853    1464    1725 
   Size:        853     611     261

  Fragments arranged by size: 

                 853     611     261
 
XmaI C'CCGG_G

Cuts at:      0     146    1725 
   Size:        146    1579
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApaI     ApoI     AvaI    AvrII
    BanII     BbsI    BciVI    BglII Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI
   BstEII    BstYI     BtgI    BtgZI     DraI   DraIII     DrdI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
     MmeI     MscI   MspA1I     NcoI     NspI     PciI    PflMI     PfoI
     PpiI    PpuMI     PsiI   PspOMI     PstI    SacII     SfcI     SmaI
     SmlI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI
     BlpI     BmtI     BplI    BsaAI    BsaHI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspHI    BsrGI   BssHII    BssSI    BstBI    BstXI
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
    PshAI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     XbaI     XhoI     XmnI     ZraI
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