Restriction map for pF1KE0796
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmI        
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AocI          670  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       716  AGCGCT               AGC^GCT
ApaI         1220  GGGCCC               GGGCC^C
Asp700I        49  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI          193  GGTGCC               G^G[CT][GA]CC
BbvCI        1084  GCTGAGG              CCTCAGC(-5/-2)
BsgI          997  GTGCAG               GTGCAG(16/14)
Bsp1407I      140  TGTACA               T^GTACA
BsrDI         495  CATTGC               GCAATG(2/0)
BsrGI         140  TGTACA               T^GTACA
BstAPI        827  GCAGGGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        670  CCTGAGG              CC^T[ACGT]AGG
DpnII         508  GATC                 ^GATC
DraII        1220  GGGCCCT              [GA]G^G[ACGT]CC[CT]
Eco47III      716  AGCGCT               AGC^GCT
Eco81I        670  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1224  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1220  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       991  CCATGG               C^C[AT][AT]GG
HindIII      1180  AAGCTT               A^AGCTT
MaeII         458  ACGT                 A^CGT
MboI          508  GATC                 ^GATC
MseI         1100  TTAA                 T^TAA
MslI          421  CACTTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1082  CAGCTG               C[AC]G^C[GT]G
NcoI          991  CCATGG               C^CATGG
NdeII         508  GATC                 ^GATC
PvuII        1082  CAGCTG               CAG^CTG
SapI         1074  GCTCTTC              GCTCTTC(1/4)
Sau3AI        508  GATC                 ^GATC
SspBI         140  TGTACA               T^GTACA
StyI          991  CCATGG               C^C[AT][AT]GG
TfiI          535  GAATC                G^A[AT]TC
Tru9I        1100  TTAA                 T^TAA
XcmI         1230  CCAGGCAGGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           49  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          141  GTAC                 GT^AC
              611  GTAC                
BanII        1220  GGGCCC               G[GA]GC[CT]^C
             1254  GAGCCC              
BbsI         1246  GAAGAC               GAAGAC(2/6)
              685  GTCTTC              
BfaI         1135  CTAG                 C^TAG
             1156  CTAG                
BpuAI        1246  GAAGAC               GAAGAC(2/6)
              685  GTCTTC              
BseRI          78  GAGGAG               GAGGAG(10/8)
             1141  GAGGAG              
BsmBI         286  GAGACG               CGTCTC(1/5)
             1215  GAGACG              
BstXI         440  CCAGCCGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              932  CCAAGCTCCTGG        
CfoI          717  GCGC                 GCG^C
              968  GCGC                
DsaI          991  CCATGG               C^C[GA][CT]GG
             1239  CCGTGG              
EarI         1075  CTCTTC               CTCTTC(1/4)
             1123  GAAGAG              
HaeII         716  AGCGCT               [GA]GCGC^[CT]
              967  AGCGCC              
HgaI          288  GACGC                GACGC(5/10)
             1071  GACGC               
HhaI          717  GCGC                 GCG^C
              968  GCGC                
HinP1I        717  GCGC                 G^CGC
              968  GCGC                
HphI           70  GGTGA                GGTGA(8/7)
             1243  GGTGA               
Ksp632I      1075  CTCTTC               CTCTTC(1/4)
             1123  GAAGAG              
MaeI         1135  CTAG                 C^TAG
             1156  CTAG                
PleI          112  GACTC                GAGTC(4/5)
             1022  GACTC               
RsaI          141  GTAC                 GT^AC
              611  GTAC                
SfaNI         949  GATGC                GCATC(5/9)
              857  GCATC               
TaqI          239  TCGA                 T^CGA
              276  TCGA                
Tsp509I       158  AATT                 ^AATT
              377  AATT                
TthHB8I       239  TCGA                 T^CGA
              276  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       ItaI        MaeIII      
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PstI        Sau96I      
ScrFI       SfcI        TseI        Tsp45I      TspRI       
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