Restriction map for pF1KB0989
 (Linear) (Six-base) MAPSORT of: KIBB0989.seq  Check: 1252  from: 1  to: 405



KIBB0989.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

ApaI G_GGCC'C

Cuts at:      0      47     237     405 
   Size:         47     190     168

  Fragments arranged by size: 

                 190     168      47
 
AvaI C'yCGr_G

Cuts at:      0      58     230     237     405 
   Size:         58     172       7     168

  Fragments arranged by size: 

                 172     168      58       7
 
BanI G'GyrC_C

Cuts at:      0      18     405 
   Size:         18     387
 
BanII G_rGCy'C

Cuts at:      0      47      65     215     237     405 
   Size:         47      18     150      22     168

  Fragments arranged by size: 

                 168     150      47      22      18
 
BlpI GC'TnA_GC

Cuts at:      0      95     382     405 
   Size:         95     287      23

  Fragments arranged by size: 

                 287      95      23
 
Bme1580I G_kGCm'C

Cuts at:      0      47     237     405 
   Size:         47     190     168

  Fragments arranged by size: 

                 190     168      47
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     405 
   Size:        229     176
 
Bpu10I CC'TnA_GC

Cuts at:      0      39     405 
   Size:         39     366
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     202     232     405 
   Size:        202      30     173

  Fragments arranged by size: 

                 202     173      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      72     405 
   Size:         72     333
 
BseYI C'CCAG_C

Cuts at:      0     116     405 
   Size:        116     289
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     405 
   Size:        272     133
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     383     405 
   Size:         65     318      22

  Fragments arranged by size: 

                 318      65      22
 
Bsp1286I G_dGCh'C

Cuts at:      0      47      65     215     237     383     405 
   Size:         47      18     150      22     146      22

  Fragments arranged by size: 

                 150     146      47      22      22      18
 
BsrBI CCG'CTC

Cuts at:      0      94     405 
   Size:         94     311
 
BstYI r'GATC_y

Cuts at:      0     338     405 
   Size:        338      67
 
EarI CTCTTCn'nnn_

Cuts at:      0     193     405 
   Size:        193     212
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     207     331     405 
   Size:        207     124      74

  Fragments arranged by size: 

                 207     124      74
 
EcoICRI GAG'CTC

Cuts at:      0      63     405 
   Size:         63     342
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     405 
   Size:        229     176
 
EcoO109I rG'GnC_Cy

Cuts at:      0      43      44      51     234     405 
   Size:         43       1       7     183     171

  Fragments arranged by size: 

                 183     171      43       7       1
 
FspI TGC'GCA

Cuts at:      0     355     405 
   Size:        355      50
 
HaeII r_GCGC'y

Cuts at:      0     206     405 
   Size:        206     199
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     202     234     307     339     405 
   Size:        202      32      73      32      66

  Fragments arranged by size: 

                 202      73      66      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     405 
   Size:         90     315
 
MspA1I CmG'CkG

Cuts at:      0      15      73      99     312     405 
   Size:         15      58      26     213      93

  Fragments arranged by size: 

                 213      93      58      26      15
 
NspI r_CATG'y

Cuts at:      0     146     405 
   Size:        146     259
 
PasI CC'CwG_GG

Cuts at:      0      47     104     405 
   Size:         47      57     301

  Fragments arranged by size: 

                 301      57      47
 
PspOMI G'GGCC_C

Cuts at:      0      43     233     405 
   Size:         43     190     172

  Fragments arranged by size: 

                 190     172      43
 
PvuII CAG'CTG

Cuts at:      0      15      99     405 
   Size:         15      84     306

  Fragments arranged by size: 

                 306      84      15
 
SacI G_AGCT'C

Cuts at:      0      65     405 
   Size:         65     340
 
SapI GCTCTTCn'nnn_

Cuts at:      0     193     405 
   Size:        193     212
 
SmaI CCC'GGG

Cuts at:      0     232     239     405 
   Size:        232       7     166

  Fragments arranged by size: 

                 232     166       7
 
TatI w'GTAC_w

Cuts at:      0     299     405 
   Size:        299     106
 
XmaI C'CCGG_G

Cuts at:      0     230     237     405 
   Size:        230       7     168

  Fragments arranged by size: 

                 230     168       7
 

 Enzymes that do cut:

     ApaI     AvaI     BanI    BanII     BlpI Bme1580I     BpmI   Bpu10I
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrBI    BstYI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FspI    HaeII    Hin4I
     MmeI   MspA1I     NspI     PasI   PspOMI    PvuII     SacI     SapI
     SmaI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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