Restriction map for pF1KB9904
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        AspEI       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BfaI        BfrI        
BlnI        BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstXI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    Eco81I      EcoRI       EcoT22I     
FseI        FspI        HaeII       HindIII     HpaI        
KasI        Ksp632I     KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        968  GGTACC               G^GTACC
AccI         1294  GTAGAC               GT^[AC][GT]AC
AccII           5  CGCG                 CG^CG
AcyI           35  GGCGTC               G[GA]^CG[CT]C
AfaI          969  GTAC                 GT^AC
AlwI         1167  GGATC                GGATC(4/5)
AlwNI        1339  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       968  GGTACC               G^GTACC
AspHI        1147  GTGCTC               G[AT]GC[AT]^C
AspI         1189  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI         840  GGATAC               GTATCC(6/5)
BclI          834  TGATCA               T^GATCA
BlpI         1753  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1753  GCTCAGC              GC^T[ACGT]AGC
BsaHI          35  GGCGTC               G[GA]^CG[CT]C
BsgI         1507  CTGCAC               GTGCAG(16/14)
BsiHKAI      1147  GTGCTC               G[AT]GC[AT]^C
BspHI        1241  TCATGA               T^CATGA
BsrFI         196  GCCGGC               [GA]^CCGG[CT]
BstUI           5  CGCG                 CG^CG
CelII        1753  GCTCAGC              GC^T[ACGT]AGC
CfoI         1620  GCGC                 GCG^C
Cfr10I        196  GCCGGC               [GA]^CCGG[CT]
EagI          162  CGGCCG               C^GGCCG
EclXI         162  CGGCCG               C^GGCCG
Eco52I        162  CGGCCG               C^GGCCG
EcoNI         351  CCTTGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         763  GATATC               GAT^ATC
FbaI          834  TGATCA               T^GATCA
HgaI           36  GCGTC                GACGC(5/10)
HhaI         1620  GCGC                 GCG^C
Hin1I          35  GGCGTC               G[GA]^CG[CT]C
HinP1I       1620  GCGC                 G^CGC
HincII        511  GTCAAC               GT[CT]^[GA]AC
HindII        511  GTCAAC               GT[CT]^[GA]AC
KpnI          968  GGTACC               GGTAC^C
MaeII        1502  ACGT                 A^CGT
MslI          979  CATTACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI            5  CGCG                 CG^CG
NaeI          196  GCCGGC               GCC^GGC
NcoI         1265  CCATGG               C^CATGG
NgoMIV        196  GCCGGC               G^CCGGC
PflFI        1189  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         1241  TCATGA               T^CATGA
RsaI          969  GTAC                 GT^AC
SfcI         1292  CTGTAG               C^T[GA][CT]AG
SgrAI         195  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI           20  CCCGGG               CCC^GGG
SmlI          181  CTCAAG               C^T[CT][GA]AG
Tth111I      1189  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          20  CCCGGG               C^CCGGG
XmaI           20  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          709  GAATTT               [GA]^AATT[CT]
             1237  AAATTC              
ApoI          709  GAATTT               [GA]^AATT[CT]
             1237  AAATTC              
Asp700I       819  GAATGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1596  GAAAGTATTC          
AvaI           20  CCCGGG               C^[CT]CG[GA]G
             1117  CCCGAG              
BanI          968  GGTACC               G^G[CT][GA]CC
             1091  GGCACC              
BglI          238  GCCGGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              730  GCCCAGCGGGC         
BglII         533  AGATCT               A^GATCT
             1556  AGATCT              
BsiEI          82  CGGTCG               CG[GA][CT]^CG
              162  CGGCCG              
BsmFI         548  GGGAC                GGGAC(10/14)
              848  GGGAC               
BsoBI          20  CCCGGG               C^[CT]CG[GA]G
             1117  CCCGAG              
BsrDI         489  CATTGC               GCAATG(2/0)
             1220  GCAATG              
BstYI         533  AGATCT               [GA]^GATC[CT]
             1556  AGATCT              
EcoT14I       740  CCAAGG               C^C[AT][AT]GG
             1265  CCATGG              
MflI          533  AGATCT               [GA]^GATC[CT]
             1556  AGATCT              
SfaNI        1660  GATGC                GCATC(5/9)
             1484  GCATC               
StuI          359  AGGCCT               AGG^CCT
              743  AGGCCT              
StyI          740  CCAAGG               C^C[AT][AT]GG
             1265  CCATGG              
TaqI          613  TCGA                 T^CGA
              794  TCGA                
TfiI         1435  GAATC                G^A[AT]TC
             1648  GAATC               
TthHB8I       613  TCGA                 T^CGA
              794  TCGA                
XcmI          380  CCATCAGCTATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1265  CCATGGTGGAGATGG     
XhoII         533  AGATCT               [GA]^GATC[CT]
             1556  AGATCT              
XmnI          819  GAATGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1596  GAAAGTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AluI        ApaI        AvaII       BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoO65I     EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       TseI        Tsp45I      
Tsp509I     TspRI       
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