Restriction map for
pF1KB9904
(Circular) (Six-base) MAPSORT of: pF1KB9904.seq Check: 2858 from: 1 to: 4880
pF1KB9904 4880 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 1403 1918 1403
Size: 515 4365
Acc65I G'GTAC_C
Cuts at: 1076 1896 1076
Size: 820 4060
AclI AA'CG_TT
Cuts at: 4720 4720
Size: 4880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 1521 1861 2705 3137 3884 4152 916
Size: 605 340 844 432 747 268 1644
Fragments arranged by size:
1644 844 747 605 432 340 268
AflIII A'CryG_T
Cuts at: 2232 3336 4313 2232
Size: 1104 977 2799
Fragments arranged by size:
2799 1104 977
AgeI A'CCGG_T
Cuts at: 3268 3268
Size: 4880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1630 1630
Size: 4880
AlwNI CAG_nnn'CTG
Cuts at: 1452 2185 3752 1452
Size: 733 1567 2580
Fragments arranged by size:
2580 1567 733
ApaI G_GGCC'C
Cuts at: 279 1067 1112 1174 279
Size: 788 45 62 3985
Fragments arranged by size:
3985 788 62 45
ApaLI G'TGCA_C
Cuts at: 3650 3650
Size: 4880
ApoI r'AATT_y
Cuts at: 817 1345 1884 4233 4489 817
Size: 528 539 2349 256 1208
Fragments arranged by size:
2349 1208 539 528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 128 1225 1900 128
Size: 1097 675 3108
Fragments arranged by size:
3108 1097 675
BamHI G'GATC_C
Cuts at: 1905 1905
Size: 4880
BanI G'GyrC_C
Cuts at: 1076 1199 1896 2310 2558 2593 1076
Size: 123 697 414 248 35 3363
Fragments arranged by size:
3363 697 414 248 123 35
BanII G_rGCy'C
Cuts at: 279 1067 1112 1174 1894 2924 279
Size: 788 45 62 720 1030 2235
Fragments arranged by size:
2235 1030 788 720 62 45
BbeI G_GCGC'C
Cuts at: 2314 2562 2314
Size: 248 4632
BbsI GAAGACnn'nnnn_
Cuts at: 1296 1305 1517 1296
Size: 9 212 4659
Fragments arranged by size:
4659 212 9
BciVI GTATCCnnnnn_n'
Cuts at: 942 2778 3539 4880 942
Size: 1836 761 1341 942
Fragments arranged by size:
1836 1341 942 761
BclI T'GATC_A
Cuts at: 942 2400 942
Size: 1458 3422
BglI GCCn_nnn'nGGC
Cuts at: 352 844 352
Size: 492 4388
BglII A'GATC_T
Cuts at: 641 1664 2395 4432 641
Size: 1023 731 2037 1089
Fragments arranged by size:
2037 1089 1023 731
BlpI GC'TnA_GC
Cuts at: 1862 1992 1862
Size: 130 4750
Bme1580I G_kGCm'C
Cuts at: 279 840 1067 1112 1174 1202 2505 2598 3654
Size: 561 227 45 62 28 1303 93 1056
Cuts at: 3654 279
Size: 1505
Fragments arranged by size:
1505 1303 1056 561 227 93 62 45
28
BmrI ACTGGGnnnn_n'
Cuts at: 406 1185 2092 2173 2508 4652 406
Size: 779 907 81 335 2144 634
Fragments arranged by size:
2144 907 779 634 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1749 3247 1749
Size: 1498 3382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 1657 1768 1795 175
Size: 1482 111 27 3260
Fragments arranged by size:
3260 1482 111 27
Bpu10I CC'TnA_GC
Cuts at: 281 281
Size: 4880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 2056 2935 3427 3725 274
Size: 1782 879 492 298 1429
Fragments arranged by size:
1782 1429 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4584 4584
Size: 4880
BsaAI yAC'GTr
Cuts at: 2233 2233
Size: 4880
BsaBI GATnn'nnATC
Cuts at: 6 2394 4431 6
Size: 2388 2037 455
Fragments arranged by size:
2388 2037 455
BsaHI Gr'CG_yC
Cuts at: 144 2311 2559 3261 4757 144
Size: 2167 248 702 1496 267
Fragments arranged by size:
2167 1496 702 267 248
BsaWI w'CCGG_w
Cuts at: 2069 2590 3268 3542 3689 4100 2069
Size: 521 678 274 147 411 2849
Fragments arranged by size:
2849 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 188 218 188
Size: 30 4850
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 356 1663 1725 356
Size: 1307 62 3511
Fragments arranged by size:
3511 1307 62
BseYI C'CCAG_C
Cuts at: 609 839 1603 1677 2305 3063 3640 4136 609
Size: 230 764 74 628 758 577 496 1353
Fragments arranged by size:
1353 764 758 628 577 496 230 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1600 1600
Size: 4880
BsiEI CG_ry'CG
Cuts at: 104 193 273 2422 2468 3676 104
Size: 89 80 2149 46 1208 1308
Fragments arranged by size:
2149 1308 1208 89 80 46
BsiHKAI G_wGCw'C
Cuts at: 1259 1894 2672 2862 3654 1259
Size: 635 778 190 792 2485
Fragments arranged by size:
2485 792 778 635 190
Bsp1286I G_dGCh'C
Cuts at: 279 840 1067 1112 1174 1202 1259 1894 2505
Size: 561 227 45 62 28 57 635 611
Cuts at: 2505 2598 2672 2862 2924 3654 279
Size: 93 74 190 62 730 1505
Fragments arranged by size:
1505 730 635 611 561 227 190 93
74 62 62 57 45 28
BspEI T'CCGG_A
Cuts at: 4100 4100
Size: 4880
BspHI T'CATG_A
Cuts at: 1349 4056 1349
Size: 2707 2173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1930 2144 2446 2827 1930
Size: 214 302 381 3983
Fragments arranged by size:
3983 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3172 3226 4710 4875 50
Size: 3122 54 1484 165 55
Fragments arranged by size:
3122 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 596 1335 2792 596
Size: 739 1457 2684
Fragments arranged by size:
2684 1457 739
BsrFI r'CCGG_y
Cuts at: 304 2878 3059 3268 304
Size: 2574 181 209 1916
Fragments arranged by size:
2574 1916 209 181
BssSI C'ACGA_G
Cuts at: 3151 3509 3151
Size: 358 4522
BstAPI GCAn_nnn'nTGC
Cuts at: 2257 2257
Size: 4880
BstBI TT'CG_AA
Cuts at: 3241 3241
Size: 4880
BstEII G'GTnAC_C
Cuts at: 604 988 1159 1641 604
Size: 384 171 482 3843
Fragments arranged by size:
3843 482 384 171
BstYI r'GATC_y
Cuts at: 1 641 1664 1905 2372 2395 2730 2976 3988
Size: 640 1023 241 467 23 335 246 1012
Cuts at: 3988 4074 4086 4432 1
Size: 86 12 346 449
Fragments arranged by size:
1023 1012 640 467 449 346 335 246
241 86 23 12
BtgI C'CryG_G
Cuts at: 728 1068 1373 728
Size: 340 305 4235
Fragments arranged by size:
4235 340 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 214 823 1685 1781 2830 3011 3174 214
Size: 609 862 96 1049 181 163 1920
Fragments arranged by size:
1920 1049 862 609 181 163 96
BtsI GCAGTG_nn'
Cuts at: 267 572 1856 2123 267
Size: 305 1284 267 3024
Fragments arranged by size:
3024 1284 305 267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 960 995 960
Size: 35 4845
DraI TTT'AAA
Cuts at: 1879 1879
Size: 4880
DrdI GACnn_nn'nnGTC
Cuts at: 2586 3444 2586
Size: 858 4022
EaeI y'GGCC_r
Cuts at: 240 270 1690 2465 2639 3030 3057 4402 240
Size: 30 1420 775 174 391 27 1345 718
Fragments arranged by size:
1420 1345 775 718 391 174 30 27
EagI C'GGCC_G
Cuts at: 270 2465 270
Size: 2195 2685
EarI CTCTTCn'nnn_
Cuts at: 2903 3113 2903
Size: 210 4670
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 148 242 3088 3398 3544 4690 148
Size: 94 2846 310 146 1146 338
Fragments arranged by size:
2846 1146 338 310 146 94
EcoICRI GAG'CTC
Cuts at: 1892 1892
Size: 4880
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 916 1521 1657 1768 1795 1861 2705 3137
Size: 741 605 136 111 27 66 844 432
Cuts at: 3137 3884 4152 175
Size: 747 268 903
Fragments arranged by size:
903 844 747 741 605 432 268 136
111 66 27
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 4880
EcoO109I rG'GnC_Cy
Cuts at: 276 429 1063 1064 1109 1170 2019 276
Size: 153 634 1 45 61 849 3137
Fragments arranged by size:
3137 849 634 153 61 45 1
EcoRI G'AATT_C
Cuts at: 1884 1884
Size: 4880
EcoRV GAT'ATC
Cuts at: 873 873
Size: 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 475 2899 475
Size: 2424 2456
FspI TGC'GCA
Cuts at: 2661 2661
Size: 4880
HaeII r_GCGC'y
Cuts at: 2314 2562 3584 4563 2314
Size: 248 1022 979 2631
Fragments arranged by size:
2631 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1741 1773 2722 2752 2754 2784 1741
Size: 32 949 30 2 30 3837
Fragments arranged by size:
3837 949 32 30 30 2
HincII GTy'rAC
Cuts at: 621 1919 621
Size: 1298 3582
KasI G'GCGC_C
Cuts at: 2310 2558 2310
Size: 248 4632
KpnI G_GTAC'C
Cuts at: 1080 1900 1080
Size: 820 4060
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1487 3551 3735 1487
Size: 2064 184 2632
Fragments arranged by size:
2632 2064 184
MscI TGG'CCA
Cuts at: 2641 2641
Size: 4880
MslI CAynn'nnrTG
Cuts at: 1091 2869 2996 1091
Size: 1778 127 2975
Fragments arranged by size:
2975 1778 127
MspA1I CmG'CkG
Cuts at: 183 843 1635 1991 2305 2665 3678 3923 183
Size: 660 792 356 314 360 1013 245 1140
Fragments arranged by size:
1140 1013 792 660 360 356 314 245
NaeI GCC'GGC
Cuts at: 306 3061 306
Size: 2755 2125
NarI GG'CG_CC
Cuts at: 2311 2559 2311
Size: 248 4632
NcoI C'CATG_G
Cuts at: 1373 1373
Size: 4880
NgoMIV G'CCGG_C
Cuts at: 304 3059 304
Size: 2755 2125
NspI r_CATG'y
Cuts at: 1933 2433 3340 1933
Size: 500 907 3473
Fragments arranged by size:
3473 907 500
PasI CC'CwG_GG
Cuts at: 652 652
Size: 4880
PciI A'CATG_T
Cuts at: 3336 3336
Size: 4880
PfoI T'CCnGG_A
Cuts at: 670 1546 670
Size: 876 4004
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4848
PspOMI G'GGCC_C
Cuts at: 275 1063 1108 1170 275
Size: 788 45 62 3985
Fragments arranged by size:
3985 788 62 45
PstI C_TGCA'G
Cuts at: 1927 2612 1927
Size: 685 4195
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4880
PvuII CAG'CTG
Cuts at: 2305 2665 2305
Size: 360 4520
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 1894 1894
Size: 4880
SalI G'TCGA_C
Cuts at: 1917 1917
Size: 4880
SapI GCTCTTCn'nnn_
Cuts at: 2903 3113 2903
Size: 210 4670
SbfI CC_TGCA'GG
Cuts at: 1927 1927
Size: 4880
ScaI AGT'ACT
Cuts at: 4397 4397
Size: 4880
SfcI C'TryA_G
Cuts at: 33 1400 1923 2608 3601 3792 33
Size: 1367 523 685 993 191 1121
Fragments arranged by size:
1367 1121 993 685 523 191
SfoI GGC'GCC
Cuts at: 2312 2560 2312
Size: 248 4632
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SgrAI Cr'CCGG_yG
Cuts at: 304 304
Size: 4880
SmaI CCC'GGG
Cuts at: 130 1902 130
Size: 1772 3108
SmlI C'TyrA_G
Cuts at: 289 2035 2950 3442 3704 289
Size: 1746 915 492 262 1465
Fragments arranged by size:
1746 1465 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 1933 2433 1933
Size: 500 4380
StuI AGG'CCT
Cuts at: 469 853 469
Size: 384 4496
StyI C'CwwG_G
Cuts at: 848 1373 2014 2368 848
Size: 525 641 354 3360
Fragments arranged by size:
3360 641 525 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 623 1313 1660 2463 2596 3055 3264 623
Size: 690 347 803 133 459 209 2239
Fragments arranged by size:
2239 803 690 459 347 209 133
TatI w'GTAC_w
Cuts at: 4395 4395
Size: 4880
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 4397 4429 188
Size: 32 4177 32 639
Fragments arranged by size:
4177 639 32 32
Tth111I GACn'n_nGTC
Cuts at: 1300 2677 1300
Size: 1377 3503
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 495 1380 2375 495
Size: 885 995 3000
Fragments arranged by size:
3000 995 885
XmaI C'CCGG_G
Cuts at: 128 1900 128
Size: 1772 3108
XmnI GAAnn'nnTTC
Cuts at: 931 1708 931
Size: 777 4103
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI ZraI