Restriction map for pF1KE0029
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# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflIII      AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BbeI        BbrPI       BcgI        
BciVI       BclI        BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsiEI       BsiWI       BsmBI       BsmI        
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeII       MluI        
MslI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PflMI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccIII       1337  TCCGGA               T^CCGGA
AgeI          527  ACCGGT               A^CCGGT
AlwNI        1166  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       304  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1196  CTCGAG               C^[CT]CG[GA]G
BanI         1308  GGTGCC               G^G[CT][GA]CC
BbsI         1480  GAAGAC               GAAGAC(2/6)
BpmI          157  CTGGAG               CTGGAG(16/14)
BpuAI        1480  GAAGAC               GAAGAC(2/6)
BseAI        1337  TCCGGA               T^CCGGA
BsgI          518  CTGCAC               GTGCAG(16/14)
BsoBI        1196  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     1224  TGTACA               T^GTACA
BspDI        1454  ATCGAT               AT^CGAT
BspEI        1337  TCCGGA               T^CCGGA
BsrGI        1224  TGTACA               T^GTACA
BstAPI        228  GCAGTTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI         1454  ATCGAT               AT^CGAT
DraII         758  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI         1539  GACCAAGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         762  CCTTGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      758  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI          43  GAATTC               G^AATTC
EcoRV         784  GATATC               GAT^ATC
HindIII      1119  AAGCTT               A^AGCTT
HphI           71  TCACC                GGTGA(8/7)
MaeIII        913  GTCAC                ^GT[ACGT]AC
MroI         1337  TCCGGA               T^CCGGA
MspA1I        175  CAGCTG               C[AC]G^C[GT]G
NcoI          431  CCATGG               C^CATGG
PaeR7I       1196  CTCGAG               C^TCGAG
PinAI         527  ACCGGT               A^CCGGT
PleI         1018  GAGTC                GAGTC(4/5)
PpuMI         758  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         175  CAGCTG               CAG^CTG
SspBI        1224  TGTACA               T^GTACA
Tsp45I        913  GTCAC                ^GT[GC]AC
XcmI          238  CCAGAATTGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1196  CTCGAG               C^TCGAG
XmnI          304  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AvaII         759  GGACC                G^G[AT]CC
             1088  GGACC               
BalI          765  TGGCCA               TGG^CCA
             1299  TGGCCA              
BamHI         427  GGATCC               G^GATCC
              696  GGATCC              
BfaI          805  CTAG                 C^TAG
             1489  CTAG                
BsaBI         490  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1049  GATTTTCATC          
BsiYI         762  CCTTGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1400  CCAGCATTGGG         
BslI          762  CCTTGGCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1400  CCAGCATTGGG         
BsmAI         278  GAGAC                GTCTC(1/5)
             1548  GTCTC               
BsmFI         758  GGGAC                GGGAC(10/14)
             1087  GGGAC               
BsrFI         527  ACCGGT               [GA]^CCGG[CT]
             1484  ACCGGC              
BstYI         427  GGATCC               [GA]^GATC[CT]
              696  GGATCC              
Cfr10I        527  ACCGGT               [GA]^CCGG[CT]
             1484  ACCGGC              
Cfr13I        759  GGACC                G^G[ACGT]CC
             1088  GGACC               
DsaI          431  CCATGG               C^C[GA][CT]GG
              646  CCACGG              
EaeI          765  TGGCCA               [CT]^GGCC[GA]
             1299  TGGCCA              
EarI         1104  CTCTTC               CTCTTC(1/4)
              983  GAAGAG              
Ksp632I      1104  CTCTTC               CTCTTC(1/4)
              983  GAAGAG              
MaeI          805  CTAG                 C^TAG
             1489  CTAG                
MamI          490  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1049  GATTTTCATC          
MfeI          422  CAATTG               C^AATTG
             1082  CAATTG              
MflI          427  GGATCC               [GA]^GATC[CT]
              696  GGATCC              
MluNI         765  TGGCCA               TGG^CCA
             1299  TGGCCA              
MscI          765  TGGCCA               TGG^CCA
             1299  TGGCCA              
MunI          422  CAATTG               C^AATTG
             1082  CAATTG              
PstI          226  CTGCAG               CTGCA^G
             1474  CTGCAG              
Sau96I        759  GGACC                G^G[ACGT]CC
             1088  GGACC               
SfaNI         865  GATGC                GCATC(5/9)
             1036  GATGC               
SfcI          226  CTGCAG               C^T[GA][CT]AG
             1474  CTGCAG              
XhoII         427  GGATCC               [GA]^GATC[CT]
              696  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AcsI        AfaI        AflII       AluI        AlwI        
ApoI        AspHI       BanII       BbvCI       BbvI        
BfrI        BmyI        BsaJI       BsaWI       BseRI       
BsiHKAI     Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       DdeI        DpnII       EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        MboI        MboII       
MnlI        MseI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        SacI        
Sau3AI      ScrFI       SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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