Restriction map for pF1KE0029
 (Linear) (Six-base) MAPSORT of: KIEE0029.seq  Check: 1571  from: 1  to: 1590



KIEE0029.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AflII C'TTAA_G

Cuts at:      0     211    1122    1326    1590 
   Size:        211     911     204     264

  Fragments arranged by size: 

                 911     264     211     204
 
AgeI A'CCGG_T

Cuts at:      0     527    1590 
   Size:        527    1063
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1171    1590 
   Size:       1171     419
 
ApoI r'AATT_y

Cuts at:      0      43     320     703    1590 
   Size:         43     277     383     887

  Fragments arranged by size: 

                 887     383     277      43
 
AvaI C'yCGr_G

Cuts at:      0    1196    1590 
   Size:       1196     394
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      95     128    1590 
   Size:         95      33    1462

  Fragments arranged by size: 

                1462      95      33
 
BamHI G'GATC_C

Cuts at:      0     427     696    1590 
   Size:        427     269     894

  Fragments arranged by size: 

                 894     427     269
 
BanI G'GyrC_C

Cuts at:      0    1308    1590 
   Size:       1308     282
 
BanII G_rGCy'C

Cuts at:      0     155     188     801    1195    1517    1533    1590 
   Size:        155      33     613     394     322      16      57

  Fragments arranged by size: 

                 613     394     322     155      57      33      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1487    1590 
   Size:       1487     103
 
BbvCI CC'TCA_GC

Cuts at:      0     995    1069    1519    1590 
   Size:        995      74     450      71

  Fragments arranged by size: 

                 995     450      74      71
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1148    1590 
   Size:       1148     442
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     953    1590 
   Size:        953     637
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178    1590 
   Size:        178    1412
 
Bpu10I CC'TnA_GC

Cuts at:      0     995    1069    1285    1519    1590 
   Size:        995      74     216     234      71

  Fragments arranged by size: 

                 995     234     216      74      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1341    1590 
   Size:       1341     249
 
BsaBI GATnn'nnATC

Cuts at:      0     494    1053    1590 
   Size:        494     559     537

  Fragments arranged by size: 

                 559     537     494
 
BsaWI w'CCGG_w

Cuts at:      0      95     527    1337    1590 
   Size:         95     432     810     253

  Fragments arranged by size: 

                 810     432     253      95
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1025    1030    1092    1507    1590 
   Size:       1025       5      62     415      83

  Fragments arranged by size: 

                1025     415      83      62       5
 
BseYI C'CCAG_C

Cuts at:      0       3     800    1590 
   Size:          3     797     790

  Fragments arranged by size: 

                 797     790       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1590 
   Size:        503    1087
 
BsiHKAI G_wGCw'C

Cuts at:      0     155     188    1517    1590 
   Size:        155      33    1329      73

  Fragments arranged by size: 

                1329     155      73      33
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     188     801    1195    1517    1533    1590 
   Size:        155      33     613     394     322      16      57

  Fragments arranged by size: 

                 613     394     322     155      57      33      16
 
BspEI T'CCGG_A

Cuts at:      0    1337    1590 
   Size:       1337     253
 
BsrFI r'CCGG_y

Cuts at:      0     527    1484    1590 
   Size:        527     957     106

  Fragments arranged by size: 

                 957     527     106
 
BsrGI T'GTAC_A

Cuts at:      0    1224    1590 
   Size:       1224     366
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     234    1590 
   Size:        234    1356
 
BstYI r'GATC_y

Cuts at:      0     427     696    1590 
   Size:        427     269     894

  Fragments arranged by size: 

                 894     427     269
 
BtgI C'CryG_G

Cuts at:      0     431     646    1590 
   Size:        431     215     944

  Fragments arranged by size: 

                 944     431     215
 
BtsI GCAGTG_nn'

Cuts at:      0     223     515    1590 
   Size:        223     292    1075

  Fragments arranged by size: 

                1075     292     223
 
ClaI AT'CG_AT

Cuts at:      0    1455    1590 
   Size:       1455     135
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1545    1590 
   Size:       1545      45
 
EaeI y'GGCC_r

Cuts at:      0     765    1299    1590 
   Size:        765     534     291

  Fragments arranged by size: 

                 765     534     291
 
EarI CTCTTCn'nnn_

Cuts at:      0     978    1110    1590 
   Size:        978     132     480

  Fragments arranged by size: 

                 978     480     132
 
EcoICRI GAG'CTC

Cuts at:      0     153     186    1515    1590 
   Size:        153      33    1329      75

  Fragments arranged by size: 

                1329     153      75      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178    1590 
   Size:        178    1412
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     766    1590 
   Size:        766     824
 
EcoO109I rG'GnC_Cy

Cuts at:      0     759    1590 
   Size:        759     831
 
EcoRI G'AATT_C

Cuts at:      0      43    1590 
   Size:         43    1547
 
EcoRV GAT'ATC

Cuts at:      0     786    1590 
   Size:        786     804
 
HindIII A'AGCT_T

Cuts at:      0    1119    1590 
   Size:       1119     471
 
MfeI C'AATT_G

Cuts at:      0     422    1082    1590 
   Size:        422     660     508

  Fragments arranged by size: 

                 660     508     422
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     943    1590 
   Size:        592     351     647

  Fragments arranged by size: 

                 647     592     351
 
MscI TGG'CCA

Cuts at:      0     767    1301    1590 
   Size:        767     534     289

  Fragments arranged by size: 

                 767     534     289
 
MspA1I CmG'CkG

Cuts at:      0     177    1590 
   Size:        177    1413
 
NcoI C'CATG_G

Cuts at:      0     431    1590 
   Size:        431    1159
 
PfoI T'CCnGG_A

Cuts at:      0     155    1590 
   Size:        155    1435
 
PpuMI rG'GwC_Cy

Cuts at:      0     759    1590 
   Size:        759     831
 
PsiI TTA'TAA

Cuts at:      0     563    1590 
   Size:        563    1027
 
PstI C_TGCA'G

Cuts at:      0     230    1478    1590 
   Size:        230    1248     112

  Fragments arranged by size: 

                1248     230     112
 
PvuII CAG'CTG

Cuts at:      0     177    1590 
   Size:        177    1413
 
SacI G_AGCT'C

Cuts at:      0     155     188    1517    1590 
   Size:        155      33    1329      73

  Fragments arranged by size: 

                1329     155      73      33
 
SfcI C'TryA_G

Cuts at:      0     226    1474    1590 
   Size:        226    1248     116

  Fragments arranged by size: 

                1248     226     116
 
SmlI C'TyrA_G

Cuts at:      0     211    1122    1196    1326    1356    1590 
   Size:        211     911      74     130      30     234

  Fragments arranged by size: 

                 911     234     211     130      74      30
 
StyI C'CwwG_G

Cuts at:      0     431     762    1151    1590 
   Size:        431     331     389     439

  Fragments arranged by size: 

                 439     431     389     331
 
TatI w'GTAC_w

Cuts at:      0    1224    1590 
   Size:       1224     366
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1332    1364    1590 
   Size:       1332      32     226

  Fragments arranged by size: 

                1332     226      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     245    1590 
   Size:        245    1345
 
XhoI C'TCGA_G

Cuts at:      0    1196    1590 
   Size:       1196     394
 
XmnI GAAnn'nnTTC

Cuts at:      0     308    1590 
   Size:        308    1282
 

 Enzymes that do cut:

    AflII     AgeI    AlwNI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbsI    BbvCI     BmrI     BplI     BpmI   Bpu10I    BpuEI
    BsaBI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI
    BsrFI    BsrGI   BstAPI    BstYI     BtgI     BtsI     ClaI     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
  HindIII     MfeI     MmeI     MscI   MspA1I     NcoI     PfoI    PpuMI
     PsiI     PstI    PvuII     SacI     SfcI     SmlI     StyI     TatI
     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BsaI    BsaAI    BsaHI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI    CspCI     DraI   DraIII     EagI     EciI     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MluI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XmaI     ZraI
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