Restriction map for pF1KE0023
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaI        ApaLI       AscI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BbvI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    FauI        
Fnu4HI      FseI        FspI        HgaI        Hin1I       
ItaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        TseI        Tth111I     
Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          994  GTAGAC               GT^[AC][GT]AC
AclI          962  AACGTT               AA^CGTT
Aor51HI      1353  AGCGCT               AGC^GCT
AseI          820  ATTAAT               AT^TAAT
AsnI          820  ATTAAT               AT^TAAT
BalI         1944  TGGCCA               TGG^CCA
BbsI         2809  GAAGAC               GAAGAC(2/6)
BciVI        1275  GTATCC               GTATCC(6/5)
BclI          975  TGATCA               T^GATCA
BpuAI        2809  GAAGAC               GAAGAC(2/6)
BsaAI         515  CACGTA               [CT]AC^GT[GA]
Bsp1407I     2616  TGTACA               T^GTACA
BspMI         597  GCAGGT               ACCTGC(4/8)
BsrGI        2616  TGTACA               T^GTACA
BstAPI         82  GCAACCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII         15  GGTCACC              G^GT[ACGT]ACC
BstPI          15  GGTCACC              G^GT[ACGT]ACC
BstXI        1314  CCAAGAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI         1944  TGGCCA               [CT]^GGCC[GA]
Eco47III     1353  AGCGCT               AGC^GCT
EcoO65I        15  GGTCACC              G^GT[ACGT]ACC
EcoRV        2274  GATATC               GAT^ATC
FbaI          975  TGATCA               T^GATCA
HincII        784  GTTAAC               GT[CT]^[GA]AC
HindII        784  GTTAAC               GT[CT]^[GA]AC
HindIII      2110  AAGCTT               A^AGCTT
HpaI          784  GTTAAC               GTT^AAC
MluNI        1944  TGGCCA               TGG^CCA
MscI         1944  TGGCCA               TGG^CCA
NdeI         1057  CATATG               CA^TATG
PshBI         820  ATTAAT               AT^TAAT
Psp1406I      962  AACGTT               AA^CGTT
PstI           52  CTGCAG               CTGCA^G
SapI         2494  GCTCTTC              GCTCTTC(1/4)
ScaI         1431  AGTACT               AGT^ACT
SspBI        2616  TGTACA               T^GTACA
XbaI         2436  TCTAGA               T^CTAGA
XcmI         2390  CCAATTTTAAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AflIII        131  ACATGT               A^C[GA][CT]GT
             2004  ACATGT              
AspHI           3  GAGCTC               G[AT]GC[AT]^C
             1779  GAGCTC              
BcgI          268  CGAAAACATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2690  GCACGTCCATCG        
BcnI           78  CCCGG                CC^[GC]GG
             2340  CCCGG               
BglII         613  AGATCT               A^GATCT
             1197  AGATCT              
BsaI         1018  GAGACC               GGTCTC(1/5)
             2714  GGTCTC              
BseRI        1331  GAGGAG               GAGGAG(10/8)
             2188  GAGGAG              
BsiHKAI         3  GAGCTC               G[AT]GC[AT]^C
             1779  GAGCTC              
BspLU11I      131  ACATGT               A^CATGT
             2004  ACATGT              
BstF5I         44  GGATG                GGATG(2/0)
             1839  GGATG               
BstNI          73  CCAGG                CC^[AT]GG
              355  CCTGG               
CfoI           58  GCGC                 GCG^C
             1354  GCGC                
EcoRII         73  CCAGG                ^CC[AT]GG
              355  CCTGG               
EcoT14I       829  CCAAGG               C^C[AT][AT]GG
             1346  CCTTGG              
EcoT22I       492  ATGCAT               ATGCA^T
             2419  ATGCAT              
FokI           44  GGATG                GGATG(9/13)
             1839  GGATG               
HaeII          57  GGCGCT               [GA]GCGC^[CT]
             1353  AGCGCT              
HapII          79  CCGG                 C^CGG
             2341  CCGG                
HhaI           58  GCGC                 GCG^C
             1354  GCGC                
HinP1I         58  GCGC                 G^CGC
             1354  GCGC                
HpaII          79  CCGG                 C^CGG
             2341  CCGG                
MspI           79  CCGG                 C^CGG
             2341  CCGG                
MvaI           73  CCAGG                CC^[AT]GG
              355  CCTGG               
NciI           78  CCCGG                CC^[GC]GG
             2340  CCCGG               
NsiI          492  ATGCAT               ATGCA^T
             2419  ATGCAT              
PleI         1185  GACTC                GAGTC(4/5)
             1702  GACTC               
SacI            3  GAGCTC               GAGCT^C
             1779  GAGCTC              
SfcI           52  CTGCAG               C^T[GA][CT]AG
             1193  CTGTAG              
SmlI          326  CTCAAG               C^T[CT][GA]AG
              609  CTTGAG              
SspI          823  AATATT               AAT^ATT
             2202  AATATT              
StyI          829  CCAAGG               C^C[AT][AT]GG
             1346  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaII       BanII       BfaI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrDI       BsrI        BssKI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRI       HaeIII      HinfI       
HphI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MwoI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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