Restriction map for pF1KE0023
 (Linear) (Six-base) MAPSORT of: KIEE0023.seq  Check: 1285  from: 1  to: 2865



KIEE0023.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AccI GT'mk_AC

Cuts at:      0     995    2865 
   Size:        995    1870
 
AclI AA'CG_TT

Cuts at:      0     963    2865 
   Size:        963    1902
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1688    1982    2539    2865 
   Size:        701     987     294     557     326

  Fragments arranged by size: 

                 987     701     557     326     294
 
AfeI AGC'GCT

Cuts at:      0    1355    2865 
   Size:       1355    1510
 
AflIII A'CryG_T

Cuts at:      0     131    2004    2865 
   Size:        131    1873     861

  Fragments arranged by size: 

                1873     861     131
 
ApoI r'AATT_y

Cuts at:      0     223     262     435     533     689    1024    1144    1501 
   Size:        223      39     173      98     156     335     120     357

Cuts at:   1501    1515    1897    2262    2562    2865 
   Size:         14     382     365     300     303

  Fragments arranged by size: 

                 382     365     357     335     303     300     223     173
                 156     120      98      39      14
 
AseI AT'TA_AT

Cuts at:      0     821    2865 
   Size:        821    2044
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1916    1949    2865 
   Size:       1916      33     916

  Fragments arranged by size: 

                1916     916      33
 
BanII G_rGCy'C

Cuts at:      0       7    1783    2602    2865 
   Size:          7    1776     819     263

  Fragments arranged by size: 

                1776     819     263       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2816    2865 
   Size:       2816      49
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     257     291    2679    2713    2865 
   Size:        257      34    2388      34     152

  Fragments arranged by size: 

                2388     257     152      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1286    2865 
   Size:       1286    1579
 
BclI T'GATC_A

Cuts at:      0     975    2865 
   Size:        975    1890
 
BfrBI ATG'CAT

Cuts at:      0     494    2421    2865 
   Size:        494    1927     444

  Fragments arranged by size: 

                1927     494     444
 
BglII A'GATC_T

Cuts at:      0     613    1197    2865 
   Size:        613     584    1668

  Fragments arranged by size: 

                1668     613     584
 
BmgBI CAC'GTC

Cuts at:      0    2693    2865 
   Size:       2693     172
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2157    2621    2865 
   Size:       2157     464     244

  Fragments arranged by size: 

                2157     464     244
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1736    2422    2453    2726    2865 
   Size:       1736     686      31     273     139

  Fragments arranged by size: 

                1736     686     273     139      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     630    2865 
   Size:        311     319    2235

  Fragments arranged by size: 

                2235     319     311
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1012    2720    2865 
   Size:       1012    1708     145

  Fragments arranged by size: 

                1708    1012     145
 
BsaAI yAC'GTr

Cuts at:      0     517    2865 
   Size:        517    2348
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1346    2203    2865 
   Size:       1346     857     662

  Fragments arranged by size: 

                1346     857     662
 
BsiHKAI G_wGCw'C

Cuts at:      0       7    1783    2865 
   Size:          7    1776    1082

  Fragments arranged by size: 

                1776    1082       7
 
Bsp1286I G_dGCh'C

Cuts at:      0       7    1783    2602    2865 
   Size:          7    1776     819     263

  Fragments arranged by size: 

                1776     819     263       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     588    2865 
   Size:        588    2277
 
BsrDI GCAATG_nn'

Cuts at:      0      86     273     413     494    2865 
   Size:         86     187     140      81    2371

  Fragments arranged by size: 

                2371     187     140      86      81
 
BsrGI T'GTAC_A

Cuts at:      0    2616    2865 
   Size:       2616     249
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      88    2865 
   Size:         88    2777
 
BstEII G'GTnAC_C

Cuts at:      0      15    2865 
   Size:         15    2850
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1321    2865 
   Size:       1321    1544
 
BstYI r'GATC_y

Cuts at:      0     613    1047    1197    2865 
   Size:        613     434     150    1668

  Fragments arranged by size: 

                1668     613     434     150
 
BtsI GCAGTG_nn'

Cuts at:      0    2187    2865 
   Size:       2187     678
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     464     499    2865 
   Size:        464      35    2366

  Fragments arranged by size: 

                2366     464      35
 
DraI TTT'AAA

Cuts at:      0    2304    2397    2801    2865 
   Size:       2304      93     404      64

  Fragments arranged by size: 

                2304     404      93      64
 
EaeI y'GGCC_r

Cuts at:      0    1944    2865 
   Size:       1944     921
 
EarI CTCTTCn'nnn_

Cuts at:      0    1619    1958    2180    2438    2501    2865 
   Size:       1619     339     222     258      63     364

  Fragments arranged by size: 

                1619     364     339     258     222      63
 
EcoICRI GAG'CTC

Cuts at:      0       5    1781    2865 
   Size:          5    1776    1084

  Fragments arranged by size: 

                1776    1084       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1688    1736    1982    2422    2453    2539    2726 
   Size:        701     987      48     246     440      31      86     187

Cuts at:   2726    2865 
   Size:        139

  Fragments arranged by size: 

                 987     701     440     246     187     139      86      48
                  31
 
EcoRI G'AATT_C

Cuts at:      0     262    2262    2562    2865 
   Size:        262    2000     300     303

  Fragments arranged by size: 

                2000     303     300     262
 
EcoRV GAT'ATC

Cuts at:      0    2276    2865 
   Size:       2276     589
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1165    2865 
   Size:       1165    1700
 
HaeII r_GCGC'y

Cuts at:      0      61    1357    2865 
   Size:         61    1296    1508

  Fragments arranged by size: 

                1508    1296      61
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1222    1254    1443    1475    2865 
   Size:       1222      32     189      32    1390

  Fragments arranged by size: 

                1390    1222     189      32      32
 
HincII GTy'rAC

Cuts at:      0     786    2865 
   Size:        786    2079
 
HindIII A'AGCT_T

Cuts at:      0    2110    2865 
   Size:       2110     755
 
HpaI GTT'AAC

Cuts at:      0     786    2865 
   Size:        786    2079
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1162    2865 
   Size:       1162    1703
 
MscI TGG'CCA

Cuts at:      0    1946    2865 
   Size:       1946     919
 
MslI CAynn'nnrTG

Cuts at:      0     278    1450    2024    2865 
   Size:        278    1172     574     841

  Fragments arranged by size: 

                1172     841     574     278
 
NdeI CA'TA_TG

Cuts at:      0    1058    2865 
   Size:       1058    1807
 
NsiI A_TGCA'T

Cuts at:      0     496    2423    2865 
   Size:        496    1927     442

  Fragments arranged by size: 

                1927     496     442
 
NspI r_CATG'y

Cuts at:      0     135    2008    2777    2865 
   Size:        135    1873     769      88

  Fragments arranged by size: 

                1873     769     135      88
 
PciI A'CATG_T

Cuts at:      0     131    2004    2865 
   Size:        131    1873     861

  Fragments arranged by size: 

                1873     861     131
 
PfoI T'CCnGG_A

Cuts at:      0    2339    2865 
   Size:       2339     526
 
PstI C_TGCA'G

Cuts at:      0      56    2865 
   Size:         56    2809
 
SacI G_AGCT'C

Cuts at:      0       7    1783    2865 
   Size:          7    1776    1082

  Fragments arranged by size: 

                1776    1082       7
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2501    2865 
   Size:       2501     364
 
ScaI AGT'ACT

Cuts at:      0    1433    2865 
   Size:       1433    1432
 
SfcI C'TryA_G

Cuts at:      0      52    1193    2865 
   Size:         52    1141    1672

  Fragments arranged by size: 

                1672    1141      52
 
SmlI C'TyrA_G

Cuts at:      0     326     609    2865 
   Size:        326     283    2256

  Fragments arranged by size: 

                2256     326     283
 
SspI AAT'ATT

Cuts at:      0     825    2204    2865 
   Size:        825    1379     661

  Fragments arranged by size: 

                1379     825     661
 
StyI C'CwwG_G

Cuts at:      0     829    1346    2865 
   Size:        829     517    1519

  Fragments arranged by size: 

                1519     829     517
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       3     692     759    2164    2865 
   Size:          3     689      67    1405     701

  Fragments arranged by size: 

                1405     701     689      67       3
 
TatI w'GTAC_w

Cuts at:      0    1431    2616    2865 
   Size:       1431    1185     249

  Fragments arranged by size: 

                1431    1185     249
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      89     121     288     320    2865 
   Size:         89      32     167      32    2545

  Fragments arranged by size: 

                2545     167      89      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2436    2865 
   Size:       2436     429
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2397    2865 
   Size:       2397     468
 
XmnI GAAnn'nnTTC

Cuts at:      0     689    1829    2289    2865 
   Size:        689    1140     460     576

  Fragments arranged by size: 

                1140     689     576     460
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AfeI   AflIII     ApoI     AseI     BaeI
    BanII     BbsI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BseRI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrGI   BstAPI   BstEII    BstXI    BstYI     BtsI
    CspCI     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoRI    EcoRV
     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     MmeI     MscI
     MslI     NdeI     NsiI     NspI     PciI     PfoI     PstI     SacI
     SapI     ScaI     SfcI     SmlI     SspI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I    AflII     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
    BamHI     BanI     BbeI    BbvCI     BglI     BlpI Bme1580I     BmrI
     BmtI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I     FseI     FspI
    FspAI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI  Tth111I     XhoI     XmaI     ZraI
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