Restriction map for pF1KE0014
 (Linear) (Six-base) MAPSORT of: KIEE0014.seq  Check: 7031  from: 1  to: 1461



KIEE0014.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AatII G_ACGT'C

Cuts at:      0     100    1461 
   Size:        100    1361
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     794    1461 
   Size:        280     514     667

  Fragments arranged by size: 

                 667     514     280
 
AflII C'TTAA_G

Cuts at:      0    1342    1461 
   Size:       1342     119
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     832     853    1461 
   Size:        832      21     608

  Fragments arranged by size: 

                 832     608      21
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     123    1461 
   Size:         75      48    1338

  Fragments arranged by size: 

                1338      75      48
 
ApaI G_GGCC'C

Cuts at:      0     602    1461 
   Size:        602     859
 
ApoI r'AATT_y

Cuts at:      0     498    1159    1461 
   Size:        498     661     302

  Fragments arranged by size: 

                 661     498     302
 
BanI G'GyrC_C

Cuts at:      0     994    1428    1461 
   Size:        994     434      33

  Fragments arranged by size: 

                 994     434      33
 
BanII G_rGCy'C

Cuts at:      0     602    1213    1461 
   Size:        602     611     248

  Fragments arranged by size: 

                 611     602     248
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     764     782    1461 
   Size:        764      18     679

  Fragments arranged by size: 

                 764     679      18
 
BbvCI CC'TCA_GC

Cuts at:      0       9    1461 
   Size:          9    1452
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     376    1129    1461 
   Size:        376     753     332

  Fragments arranged by size: 

                 753     376     332
 
BclI T'GATC_A

Cuts at:      0     131    1461 
   Size:        131    1330
 
BfrBI ATG'CAT

Cuts at:      0     884    1461 
   Size:        884     577
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1033    1461 
   Size:       1033     428
 
BlpI GC'TnA_GC

Cuts at:      0      76     739    1461 
   Size:         76     663     722

  Fragments arranged by size: 

                 722     663      76
 
Bme1580I G_kGCm'C

Cuts at:      0     602    1461 
   Size:        602     859
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     597    1461 
   Size:        597     864
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1231    1461 
   Size:       1231     230
 
Bpu10I CC'TnA_GC

Cuts at:      0       9    1219    1387    1461 
   Size:          9    1210     168      74

  Fragments arranged by size: 

                1210     168      74       9
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676    1042    1461 
   Size:        676     366     419

  Fragments arranged by size: 

                 676     419     366
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     190    1096    1461 
   Size:        190     906     365

  Fragments arranged by size: 

                 906     365     190
 
BsaAI yAC'GTr

Cuts at:      0     535    1328    1461 
   Size:        535     793     133

  Fragments arranged by size: 

                 793     535     133
 
BsaHI Gr'CG_yC

Cuts at:      0       4      97    1461 
   Size:          4      93    1364

  Fragments arranged by size: 

                1364      93       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     757    1461 
   Size:        757     704
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655    1111    1461 
   Size:        655     456     350

  Fragments arranged by size: 

                 655     456     350
 
Bsp1286I G_dGCh'C

Cuts at:      0     602    1213    1461 
   Size:        602     611     248

  Fragments arranged by size: 

                 611     602     248
 
BsrDI GCAATG_nn'

Cuts at:      0     998    1430    1461 
   Size:        998     432      31

  Fragments arranged by size: 

                 998     432      31
 
BsrFI r'CCGG_y

Cuts at:      0     400    1461 
   Size:        400    1061
 
BsrGI T'GTAC_A

Cuts at:      0     812     842    1461 
   Size:        812      30     619

  Fragments arranged by size: 

                 812     619      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1125    1461 
   Size:       1125     336
 
BstEII G'GTnAC_C

Cuts at:      0     151    1461 
   Size:        151    1310
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147    1461 
   Size:        147    1314
 
BtsI GCAGTG_nn'

Cuts at:      0     332     392     733    1461 
   Size:        332      60     341     728

  Fragments arranged by size: 

                 728     341     332      60
 
DraIII CAC_nnn'GTG

Cuts at:      0     436    1461 
   Size:        436    1025
 
EaeI y'GGCC_r

Cuts at:      0    1229    1371    1461 
   Size:       1229     142      90

  Fragments arranged by size: 

                1229     142      90
 
EarI CTCTTCn'nnn_

Cuts at:      0     220    1294    1327    1461 
   Size:        220    1074      33     134

  Fragments arranged by size: 

                1074     220     134      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     794    1231    1461 
   Size:        280     514     437     230

  Fragments arranged by size: 

                 514     437     280     230
 
EcoO109I rG'GnC_Cy

Cuts at:      0     584     598     599    1461 
   Size:        584      14       1     862

  Fragments arranged by size: 

                 862     584      14       1
 
EcoRI G'AATT_C

Cuts at:      0     498    1159    1461 
   Size:        498     661     302

  Fragments arranged by size: 

                 661     498     302
 
EcoRV GAT'ATC

Cuts at:      0     414    1461 
   Size:        414    1047
 
HaeII r_GCGC'y

Cuts at:      0      43     124     699    1461 
   Size:         43      81     575     762

  Fragments arranged by size: 

                 762     575      81      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1127    1159    1461 
   Size:       1127      32     302

  Fragments arranged by size: 

                1127     302      32
 
HincII GTy'rAC

Cuts at:      0     471    1461 
   Size:        471     990
 
MfeI C'AATT_G

Cuts at:      0     239    1461 
   Size:        239    1222
 
MscI TGG'CCA

Cuts at:      0    1373    1461 
   Size:       1373      88
 
MslI CAynn'nnrTG

Cuts at:      0     814     958    1461 
   Size:        814     144     503

  Fragments arranged by size: 

                 814     503     144
 
MspA1I CmG'CkG

Cuts at:      0      13     806    1461 
   Size:         13     793     655

  Fragments arranged by size: 

                 793     655      13
 
NsiI A_TGCA'T

Cuts at:      0     886    1461 
   Size:        886     575
 
NspI r_CATG'y

Cuts at:      0     813     819    1461 
   Size:        813       6     642

  Fragments arranged by size: 

                 813     642       6
 
PasI CC'CwG_GG

Cuts at:      0     102    1461 
   Size:        102    1359
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1028    1283    1461 
   Size:       1028     255     178

  Fragments arranged by size: 

                1028     255     178
 
PfoI T'CCnGG_A

Cuts at:      0      68    1461 
   Size:         68    1393
 
PmlI CAC'GTG

Cuts at:      0    1328    1461 
   Size:       1328     133
 
PspOMI G'GGCC_C

Cuts at:      0     598    1461 
   Size:        598     863
 
PstI C_TGCA'G

Cuts at:      0      59    1461 
   Size:         59    1402
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1294    1461 
   Size:       1294     167
 
SexAI A'CCwGG_T

Cuts at:      0    1139    1461 
   Size:       1139     322
 
SfcI C'TryA_G

Cuts at:      0      55    1461 
   Size:         55    1406
 
SmlI C'TyrA_G

Cuts at:      0     691    1057    1342    1461 
   Size:        691     366     285     119

  Fragments arranged by size: 

                 691     366     285     119
 
StuI AGG'CCT

Cuts at:      0     233    1461 
   Size:        233    1228
 
StyI C'CwwG_G

Cuts at:      0    1065    1368    1432    1461 
   Size:       1065     303      64      29

  Fragments arranged by size: 

                1065     303      64      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1020    1461 
   Size:       1020     441
 
TatI w'GTAC_w

Cuts at:      0     812     842    1461 
   Size:        812      30     619

  Fragments arranged by size: 

                 812     619      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1029    1461 
   Size:       1029     432
 
ZraI GAC'GTC

Cuts at:      0      98    1461 
   Size:         98    1363
 

 Enzymes that do cut:

    AatII     AcuI    AflII     AlfI    AlwNI     ApaI     ApoI     BanI
    BanII     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BseRI     BsgI Bsp1286I    BsrDI    BsrFI    BsrGI   BstAPI   BstEII
    BstXI     BtsI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI
    EcoRV    HaeII    Hin4I   HincII     MfeI     MscI     MslI   MspA1I
     NsiI     NspI     PasI    PflMI     PfoI     PmlI   PspOMI     PstI
     SapI    SexAI     SfcI     SmlI     StuI     StyI    TaqII     TatI
     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BcgI    BglII    BmgBI     BmtI     BplI
    BsaBI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI   BssHII    BssSI    BstBI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EciI  EcoICRI    EcoNI     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI
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