Restriction map for pF1KA1460
##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       AccII       AflII       AgeI        AhdI        
Aor51HI     ApaI        AscI        AspEI       AvrII       
BbeI        BfrI        BlnI        BlpI        Bpu1102I    
BsiWI       BsmBI       BsrBI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       Eam1105I    Eco47III    EcoO65I     EcoRV       
EcoT22I     FseI        HaeII       KasI        KspI        
MfeI        MluI        MunI        MvnI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PstI        
PvuI        SacI        SacII       SapI        SbfI        
SexAI       SfoI        SfuI        SgrAI       SnaBI       
Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       1252  GGTACC               G^GTACC
AccIII        738  TCCGGA               T^CCGGA
AclI         5044  AACGTT               AA^CGTT
Alw44I       3914  GTGCAC               G^TGCAC
ApaLI        3914  GTGCAC               G^TGCAC
Asp700I      4449  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1252  GGTACC               G^GTACC
AviII        5390  TGCGCA               TGC^GCA
BamHI         610  GGATCC               G^GATCC
BcgI         4362  GCAACAATCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        4235  AGATCT               A^GATCT
BseAI         738  TCCGGA               T^CCGGA
Bsp1407I     3663  TGTACA               T^GTACA
BspDI        5374  ATCGAT               AT^CGAT
BspEI         738  TCCGGA               T^CCGGA
BspHI        5142  TCATGA               T^CATGA
BspMI        2361  ACCTGC               ACCTGC(4/8)
BsrGI        3663  TGTACA               T^GTACA
BssSI         398  CTCGTG               CACGAG(-5/-1)
ClaI         5374  ATCGAT               AT^CGAT
CpoI         5680  CGGACCG              CG^G[AT]CCG
DraI         3489  TTTAAA               TTT^AAA
DraIII       5394  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         4309  CGGCCG               C^GGCCG
EclXI        4309  CGGCCG               C^GGCCG
Eco52I       4309  CGGCCG               C^GGCCG
EcoNI        2896  CCTGGTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         5390  TGCGCA               TGC^GCA
HindIII      3895  AAGCTT               A^AGCTT
HpaI         1693  GTTAAC               GTT^AAC
KpnI         1252  GGTACC               GGTAC^C
MroI          738  TCCGGA               T^CCGGA
NaeI         5639  GCCGGC               GCC^GGC
NgoMIV       5639  GCCGGC               G^CCGGC
NheI         1090  GCTAGC               G^CTAGC
Psp1406I     5044  AACGTT               AA^CGTT
RcaI         5142  TCATGA               T^CATGA
RsrII        5680  CGGACCG              CG^G[AT]CCG
SalI         4331  GTCGAC               G^TCGAC
SfiI         1606  GGCCCTAATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         4031  CCCGGG               CCC^GGG
SspBI        3663  TGTACA               T^GTACA
XmaCI        4031  CCCGGG               C^CCGGG
XmaI         4031  CCCGGG               C^CCGGG
XmnI         4449  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI         4331  GTCGAC               GT^[AC][GT]AC
             5232  GTCTAC              
AcyI         2039  GACGCC               G[GA]^CG[CT]C
             5284  GACGCC              
AlwNI         722  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             5590  CAGTCTCTG           
AocI         4117  CCTCAGG              CC^T[ACGT]AGG
             4953  CCTCAGG             
AseI         1171  ATTAAT               AT^TAAT
             4927  ATTAAT              
AsnI         1171  ATTAAT               AT^TAAT
             4927  ATTAAT              
AspI         4462  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5103  GACATGGTC           
BbsI          645  GTCTTC               GAAGAC(2/6)
             3804  GTCTTC              
BciVI        2202  GGATAC               GTATCC(6/5)
              393  GTATCC              
BclI         2094  TGATCA               T^GATCA
             5420  TGATCA              
BpuAI         645  GTCTTC               GAAGAC(2/6)
             3804  GTCTTC              
BsaHI        2039  GACGCC               G[GA]^CG[CT]C
             5284  GACGCC              
BsiEI        3761  CGGTCG               CG[GA][CT]^CG
             4309  CGGCCG              
BsrFI        4307  ACCGGC               [GA]^CCGG[CT]
             5639  GCCGGC              
BstAPI       4353  GCAAATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5393  GCACTCTGTGC         
Bsu36I       4117  CCTCAGG              CC^T[ACGT]AGG
             4953  CCTCAGG             
Cfr10I       4307  ACCGGC               [GA]^CCGG[CT]
             5639  GCCGGC              
DrdI         4885  GACCCTTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5207  GACTGACTGGTC        
Eco81I       4117  CCTCAGG              CC^T[ACGT]AGG
             4953  CCTCAGG             
EcoRI        1083  GAATTC               G^AATTC
             3969  GAATTC              
FbaI         2094  TGATCA               T^GATCA
             5420  TGATCA              
HgaI         2039  GACGC                GACGC(5/10)
             5284  GACGC               
Hin1I        2039  GACGCC               G[GA]^CG[CT]C
             5284  GACGCC              
PflFI        4462  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5103  GACATGGTC           
PshAI         638  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3256  GACAGTGGTC          
PshBI        1171  ATTAAT               AT^TAAT
             4927  ATTAAT              
SpeI         2677  ACTAGT               A^CTAGT
             5008  ACTAGT              
SphI         4037  GCATGC               GCATG^C
             5402  GCATGC              
StuI          380  AGGCCT               AGG^CCT
             2252  AGGCCT              
Tth111I      4462  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5103  GACATGGTC           
XcmI         3941  CCATAGCAGGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5299  CCAGGTTCCAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbrPI       BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaAI       BsaBI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspLU11I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PmaCI       
PmlI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com