Restriction map for pF1KB5713
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspMI       BsrBI       
BsrDI       BsrFI       BssHII      BssSI       BstEII      
BstPI       Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MspA1I      
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RsrII       
SacII       SalI        SapI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII        1541  CGCG                 CG^CG
AflIII        147  ACATGT               A^C[GA][CT]GT
Alw44I       2227  GTGCAC               G^TGCAC
ApaLI        2227  GTGCAC               G^TGCAC
AvaII          39  GGACC                G^G[AT]CC
BalI         1775  TGGCCA               TGG^CCA
BcnI           36  CCCGG                CC^[GC]GG
BglII         407  AGATCT               A^GATCT
BpmI         2092  CTGGAG               CTGGAG(16/14)
BsmFI        1594  GGGAC                GGGAC(10/14)
BsmI          859  GAATGC               GAATGC(1/-1)
Bsp1407I     2280  TGTACA               T^GTACA
BspLU11I      147  ACATGT               A^CATGT
BsrGI        2280  TGTACA               T^GTACA
Bst1107I     1859  GTATAC               GTA^TAC
BstAPI       1147  GCACATATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1192  TTCGAA               TT^CGAA
BstUI        1541  CGCG                 CG^CG
BstYI         407  AGATCT               [GA]^GATC[CT]
BstZ17I      1859  GTATAC               GTA^TAC
DraI         2101  TTTAAA               TTT^AAA
DsaI         1947  CCGTGG               C^C[GA][CT]GG
EcoNI        2043  CCTCCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII          37  CCGG                 C^CGG
HincII        490  GTCAAC               GT[CT]^[GA]AC
HindII        490  GTCAAC               GT[CT]^[GA]AC
HpaII          37  CCGG                 C^CGG
MaeII         226  ACGT                 A^CGT
MflI          407  AGATCT               [GA]^GATC[CT]
MluNI        1775  TGGCCA               TGG^CCA
MscI         1775  TGGCCA               TGG^CCA
MspI           37  CCGG                 C^CGG
MvnI         1541  CGCG                 CG^CG
NciI           36  CCCGG                CC^[GC]GG
NspI          147  ACATGT               [GA]CATG^[CT]
NspV         1192  TTCGAA               TT^CGAA
PstI         2047  CTGCAG               CTGCA^G
SacI         1973  GAGCTC               GAGCT^C
SbfI         2046  CCTGCAGG             CCTGCA^GG
ScaI          833  AGTACT               AGT^ACT
SfuI         1192  TTCGAA               TT^CGAA
Sse8387I     2046  CCTGCAGG             CCTGCA^GG
SspBI        2280  TGTACA               T^GTACA
SspI          636  AATATT               AAT^ATT
Tsp45I       1169  GTCAC                ^GT[GC]AC
XhoII         407  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          793  GTCTAC               GT^[AC][GT]AC
             1859  GTATAC              
AlwI         1483  GATCC                GGATC(4/5)
             1387  GGATC               
AlwNI         295  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2007  CAGGGGCTG           
Asp700I      1185  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2134  GAAATGATTC          
AspHI        1973  GAGCTC               G[AT]GC[AT]^C
             2227  GTGCAC              
BbvCI         109  GCTGAGG              CCTCAGC(-5/-2)
             1348  GCTGAGG             
BfaI          589  CTAG                 C^TAG
             1417  CTAG                
BsaI          272  GAGACC               GGTCTC(1/5)
             1399  GAGACC              
BseRI        1526  CTCCTC               GAGGAG(10/8)
             1714  GAGGAG              
BsgI         1046  GTGCAG               GTGCAG(16/14)
             1337  GTGCAG              
BsiHKAI      1973  GAGCTC               G[AT]GC[AT]^C
             2227  GTGCAC              
BspHI        1308  TCATGA               T^CATGA
             1511  TCATGA              
BstXI         721  CCAGCCCTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2232  CCATCCAGGTGG        
Cfr13I         39  GGACC                G^G[ACGT]CC
               55  GGCCC               
EaeI          718  CGGCCA               [CT]^GGCC[GA]
             1775  TGGCCA              
HaeII          12  AGCGCC               [GA]GCGC^[CT]
               60  AGCGCC              
MaeI          589  CTAG                 C^TAG
             1417  CTAG                
MaeIII        334  GTTAC                ^GT[ACGT]AC
             1169  GTCAC               
PleI         1806  GAGTC                GAGTC(4/5)
             1986  GAGTC               
RcaI         1308  TCATGA               T^CATGA
             1511  TCATGA              
Sau96I         39  GGACC                G^G[ACGT]CC
               55  GGCCC               
SfaNI         106  GATGC                GCATC(5/9)
             1111  GCATC               
SmlI         1180  CTCAAG               C^T[CT][GA]AG
             1895  CTCAAG              
TaqI         1193  TCGA                 T^CGA
             2307  TCGA                
TthHB8I      1193  TCGA                 T^CGA
             2307  TCGA                
XmnI         1185  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2134  GAAATGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BanII       BbsI        BbvI        BmyI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        DdeI        DpnII       EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HindIII     HinfI       HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      ScrFI       SfcI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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