Restriction map for pF1KB5713
 (Linear) (Six-base) MAPSORT of: KIBB5713.seq  Check: 6605  from: 1  to: 2355



KIBB5713.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AccI GT'mk_AC

Cuts at:      0     794    1860    2355 
   Size:        794    1066     495

  Fragments arranged by size: 

                1066     794     495
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     494    1460    2271    2355 
   Size:        449      45     966     811      84

  Fragments arranged by size: 

                 966     811     449      84      45
 
AflIII A'CryG_T

Cuts at:      0     147    2355 
   Size:        147    2208
 
AleI CACnn'nnGTG

Cuts at:      0    2224    2355 
   Size:       2224     131
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     876    2355 
   Size:        876    1479
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2027    2059    2355 
   Size:       2027      32     296

  Fragments arranged by size: 

                2027     296      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     300    2012    2355 
   Size:        300    1712     343

  Fragments arranged by size: 

                1712     343     300
 
ApaLI G'TGCA_C

Cuts at:      0    2227    2355 
   Size:       2227     128
 
ApoI r'AATT_y

Cuts at:      0     189     611     685     989    1054    2098    2326    2355 
   Size:        189     422      74     304      65    1044     228      29

  Fragments arranged by size: 

                1044     422     304     228     189      74      65      29
            
 
BanII G_rGCy'C

Cuts at:      0      37     123    1977    2355 
   Size:         37      86    1854     378

  Fragments arranged by size: 

                1854     378      86      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     437     482    1202    1327    1448    1730    1929    1945 
   Size:        437      45     720     125     121     282     199      16

Cuts at:   1945    2355 
   Size:        410

  Fragments arranged by size: 

                 720     437     410     282     199     125     121      45
                  16
 
BbvCI CC'TCA_GC

Cuts at:      0     110    1349    2355 
   Size:        110    1239    1006

  Fragments arranged by size: 

                1239    1006     110
 
BglII A'GATC_T

Cuts at:      0     407    2355 
   Size:        407    1948
 
Bme1580I G_kGCm'C

Cuts at:      0    2231    2355 
   Size:       2231     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1641    1811    2355 
   Size:       1641     170     544

  Fragments arranged by size: 

                1641     544     170
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     210    2355 
   Size:        210    2145
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2113    2355 
   Size:       2113     242
 
Bpu10I CC'TnA_GC

Cuts at:      0     110    1349    2355 
   Size:        110    1239    1006

  Fragments arranged by size: 

                1239    1006     110
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1165    1880    2355 
   Size:       1165     715     475

  Fragments arranged by size: 

                1165     715     475
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     266    1393    2355 
   Size:        266    1127     962

  Fragments arranged by size: 

                1127     962     266
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1514    1544    2027    2057    2355 
   Size:       1514      30     483      30     298

  Fragments arranged by size: 

                1514     483     298      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1517    1729    2355 
   Size:       1517     212     626

  Fragments arranged by size: 

                1517     626     212
 
BseYI C'CCAG_C

Cuts at:      0      57    1296    2355 
   Size:         57    1239    1059

  Fragments arranged by size: 

                1239    1059      57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1067    1358    2355 
   Size:       1067     291     997

  Fragments arranged by size: 

                1067     997     291
 
BsiHKAI G_wGCw'C

Cuts at:      0    1977    2231    2355 
   Size:       1977     254     124

  Fragments arranged by size: 

                1977     254     124
 
BsmI GAATG_Cn'

Cuts at:      0     865    2355 
   Size:        865    1490
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     123    1977    2231    2355 
   Size:         37      86    1854     254     124

  Fragments arranged by size: 

                1854     254     124      86      37
 
BspHI T'CATG_A

Cuts at:      0    1308    1511    2355 
   Size:       1308     203     844

  Fragments arranged by size: 

                1308     844     203
 
BsrGI T'GTAC_A

Cuts at:      0    2280    2355 
   Size:       2280      75
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1153    2355 
   Size:       1153    1202
 
BstBI TT'CG_AA

Cuts at:      0    1193    2355 
   Size:       1193    1162
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     728    2239    2355 
   Size:        728    1511     116

  Fragments arranged by size: 

                1511     728     116
 
BstYI r'GATC_y

Cuts at:      0     407    2355 
   Size:        407    1948
 
BstZ17I GTA'TAC

Cuts at:      0    1861    2355 
   Size:       1861     494
 
BtgI C'CryG_G

Cuts at:      0    1947    2355 
   Size:       1947     408
 
BtsI GCAGTG_nn'

Cuts at:      0     599    1434    2355 
   Size:        599     835     921

  Fragments arranged by size: 

                 921     835     599
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1929    1964    2355 
   Size:       1929      35     391

  Fragments arranged by size: 

                1929     391      35
 
DraI TTT'AAA

Cuts at:      0    2103    2355 
   Size:       2103     252
 
EaeI y'GGCC_r

Cuts at:      0     718    1775    2355 
   Size:        718    1057     580

  Fragments arranged by size: 

                1057     718     580
 
EarI CTCTTCn'nnn_

Cuts at:      0     349     983    1040    1244    1691    2355 
   Size:        349     634      57     204     447     664

  Fragments arranged by size: 

                 664     634     447     349     204      57
 
EcoICRI GAG'CTC

Cuts at:      0    1975    2355 
   Size:       1975     380
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     494    1460    2113    2271    2355 
   Size:        449      45     966     653     158      84

  Fragments arranged by size: 

                 966     653     449     158      84      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2047    2355 
   Size:       2047     308
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1206    2355 
   Size:       1206    1149
 
HaeII r_GCGC'y

Cuts at:      0      16      64    2355 
   Size:         16      48    2291

  Fragments arranged by size: 

                2291      48      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     348     380     392     424     456     958     990    1246 
   Size:        348      32      12      32      32     502      32     256

Cuts at:   1246    1278    1514    1546    1971    2003    2355 
   Size:         32     236      32     425      32     352

  Fragments arranged by size: 

                 502     425     352     348     256     236      32      32
                  32      32      32      32      32      12
 
HincII GTy'rAC

Cuts at:      0     492    2355 
   Size:        492    1863
 
HindIII A'AGCT_T

Cuts at:      0     566     755    2296    2355 
   Size:        566     189    1541      59

  Fragments arranged by size: 

                1541     566     189      59
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     523    1054    1587    2355 
   Size:        523     531     533     768

  Fragments arranged by size: 

                 768     533     531     523
 
MscI TGG'CCA

Cuts at:      0    1777    2355 
   Size:       1777     578
 
MslI CAynn'nnrTG

Cuts at:      0     788    1643    2224    2237    2355 
   Size:        788     855     581      13     118

  Fragments arranged by size: 

                 855     788     581     118      13
 
NspI r_CATG'y

Cuts at:      0     151    2355 
   Size:        151    2204
 
PciI A'CATG_T

Cuts at:      0     147    2355 
   Size:        147    2208
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2027    2059    2355 
   Size:       2027      32     296

  Fragments arranged by size: 

                2027     296      32
 
PsiI TTA'TAA

Cuts at:      0    1822    2276    2355 
   Size:       1822     454      79

  Fragments arranged by size: 

                1822     454      79
 
PstI C_TGCA'G

Cuts at:      0    2051    2355 
   Size:       2051     304
 
SacI G_AGCT'C

Cuts at:      0    1977    2355 
   Size:       1977     378
 
SbfI CC_TGCA'GG

Cuts at:      0    2051    2355 
   Size:       2051     304
 
ScaI AGT'ACT

Cuts at:      0     835    2355 
   Size:        835    1520
 
SfcI C'TryA_G

Cuts at:      0     674    1547    2047    2355 
   Size:        674     873     500     308

  Fragments arranged by size: 

                 873     674     500     308
 
SmlI C'TyrA_G

Cuts at:      0    1180    1895    2355 
   Size:       1180     715     460

  Fragments arranged by size: 

                1180     715     460
 
SspI AAT'ATT

Cuts at:      0     638    2355 
   Size:        638    1717
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1799    2355 
   Size:       1799     556
 
TatI w'GTAC_w

Cuts at:      0     125     833    2280    2355 
   Size:        125     708    1447      75

  Fragments arranged by size: 

                1447     708     125      75
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     597     629    1263    1295    2355 
   Size:        597      32     634      32    1060

  Fragments arranged by size: 

                1060     634     597      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1189    2138    2355 
   Size:       1189     949     217

  Fragments arranged by size: 

                1189     949     217
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI    BanII     BbsI    BbvCI    BglII Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BsrGI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I     BtgI     BtsI    CspCI     DraI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI     FalI    HaeII    Hin4I   HincII  HindIII
     MmeI     MscI     MslI     NspI     PciI     PpiI     PsiI     PstI
     SacI     SbfI     ScaI     SfcI     SmlI     SspI    TaqII     TatI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstEII   Bsu36I    BtgZI     ClaI   DraIII     DrdI     EagI     EciI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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