Restriction map for pF1KB9977
 (Linear) (Six-base) MAPSORT of: KIBB9977.seq  Check: 6933  from: 1  to: 1671



KIBB9977.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      64    1671 
   Size:         64    1607
 
AccI GT'mk_AC

Cuts at:      0     113    1671 
   Size:        113    1558
 
AclI AA'CG_TT

Cuts at:      0     887    1671 
   Size:        887     784
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     503     977    1671 
   Size:        431      72     474     694

  Fragments arranged by size: 

                 694     474     431      72
 
AleI CACnn'nnGTG

Cuts at:      0    1078    1671 
   Size:       1078     593
 
ApoI r'AATT_y

Cuts at:      0     563     902    1307    1325    1671 
   Size:        563     339     405      18     346

  Fragments arranged by size: 

                 563     405     346     339      18
 
BanI G'GyrC_C

Cuts at:      0      48    1671 
   Size:         48    1623
 
BbeI G_GCGC'C

Cuts at:      0      52    1671 
   Size:         52    1619
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1368    1392    1671 
   Size:       1368      24     279

  Fragments arranged by size: 

                1368     279      24
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      15      49    1671 
   Size:         15      34    1622

  Fragments arranged by size: 

                1622      34      15
 
BfrBI ATG'CAT

Cuts at:      0    1388    1671 
   Size:       1388     283
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     212     861    1671 
   Size:        212     649     810

  Fragments arranged by size: 

                 810     649     212
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     369    1461    1671 
   Size:        369    1092     210

  Fragments arranged by size: 

                1092     369     210
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     986    1671 
   Size:        374     612     685

  Fragments arranged by size: 

                 685     612     374
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259    1401    1671 
   Size:        259    1142     270

  Fragments arranged by size: 

                1142     270     259
 
BsaBI GATnn'nnATC

Cuts at:      0     433    1565    1671 
   Size:        433    1132     106

  Fragments arranged by size: 

                1132     433     106
 
BsaHI Gr'CG_yC

Cuts at:      0      49    1671 
   Size:         49    1622
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      86    1671 
   Size:         86    1585
 
BsiHKAI G_wGCw'C

Cuts at:      0      38    1056    1224    1671 
   Size:         38    1018     168     447

  Fragments arranged by size: 

                1018     447     168      38
 
Bsp1286I G_dGCh'C

Cuts at:      0      38    1056    1224    1671 
   Size:         38    1018     168     447

  Fragments arranged by size: 

                1018     447     168      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      64    1671 
   Size:         64    1607
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1342    1671 
   Size:       1342     329
 
BstYI r'GATC_y

Cuts at:      0    1018    1671 
   Size:       1018     653
 
Bsu36I CC'TnA_GG

Cuts at:      0     139    1671 
   Size:        139    1532
 
BtgI C'CryG_G

Cuts at:      0      44      52    1229    1671 
   Size:         44       8    1177     442

  Fragments arranged by size: 

                1177     442      44       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     170    1671 
   Size:        170    1501
 
BtsI GCAGTG_nn'

Cuts at:      0    1260    1671 
   Size:       1260     411
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     760     795    1671 
   Size:        760      35     876

  Fragments arranged by size: 

                 876     760      35
 
DraI TTT'AAA

Cuts at:      0     797    1671 
   Size:        797     874
 
EarI CTCTTCn'nnn_

Cuts at:      0     522     953    1671 
   Size:        522     431     718

  Fragments arranged by size: 

                 718     522     431
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     431     503     977     986    1671 
   Size:        374      57      72     474       9     685

  Fragments arranged by size: 

                 685     474     374      72      57       9
 
HaeII r_GCGC'y

Cuts at:      0      52    1671 
   Size:         52    1619
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     372     404    1671 
   Size:        372      32    1267

  Fragments arranged by size: 

                1267     372      32
 
HincII GTy'rAC

Cuts at:      0     114     351    1671 
   Size:        114     237    1320

  Fragments arranged by size: 

                1320     237     114
 
HindIII A'AGCT_T

Cuts at:      0     483     986    1671 
   Size:        483     503     685

  Fragments arranged by size: 

                 685     503     483
 
KasI G'GCGC_C

Cuts at:      0      48    1671 
   Size:         48    1623
 
MfeI C'AATT_G

Cuts at:      0    1529    1671 
   Size:       1529     142
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1302    1671 
   Size:       1302     369
 
MslI CAynn'nnrTG

Cuts at:      0    1078    1671 
   Size:       1078     593
 
NarI GG'CG_CC

Cuts at:      0      49    1671 
   Size:         49    1622
 
NcoI C'CATG_G

Cuts at:      0      52    1229    1671 
   Size:         52    1177     442

  Fragments arranged by size: 

                1177     442      52
 
NdeI CA'TA_TG

Cuts at:      0     753    1671 
   Size:        753     918
 
NsiI A_TGCA'T

Cuts at:      0    1390    1671 
   Size:       1390     281
 
PfoI T'CCnGG_A

Cuts at:      0     441     807    1085    1671 
   Size:        441     366     278     586

  Fragments arranged by size: 

                 586     441     366     278
 
PsiI TTA'TAA

Cuts at:      0     569    1671 
   Size:        569    1102
 
SalI G'TCGA_C

Cuts at:      0     112    1671 
   Size:        112    1559
 
SfcI C'TryA_G

Cuts at:      0     642    1535    1654    1671 
   Size:        642     893     119      17

  Fragments arranged by size: 

                 893     642     119      17
 
SfoI GGC'GCC

Cuts at:      0      50    1671 
   Size:         50    1621
 
SmlI C'TyrA_G

Cuts at:      0     274    1380    1671 
   Size:        274    1106     291

  Fragments arranged by size: 

                1106     291     274
 
SpeI A'CTAG_T

Cuts at:      0     247    1671 
   Size:        247    1424
 
SspI AAT'ATT

Cuts at:      0     243     546    1671 
   Size:        243     303    1125

  Fragments arranged by size: 

                1125     303     243
 
StyI C'CwwG_G

Cuts at:      0      52    1229    1671 
   Size:         52    1177     442

  Fragments arranged by size: 

                1177     442      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      45     221    1671 
   Size:         45     176    1450

  Fragments arranged by size: 

                1450     176      45
 
TatI w'GTAC_w

Cuts at:      0     396     651    1671 
   Size:        396     255    1020

  Fragments arranged by size: 

                1020     396     255
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     621     653    1671 
   Size:        621      32    1018

  Fragments arranged by size: 

                1018     621      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1375    1611    1671 
   Size:       1375     236      60

  Fragments arranged by size: 

                1375     236      60
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AleI     ApoI     BanI     BbeI
     BbsI     BcgI    BfrBI     BmrI     BplI     BpmI    BpuEI    BsaBI
    BsaHI    BseRI  BsiHKAI Bsp1286I    BspMI   BstAPI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI     EarI  Eco57MI    HaeII
    Hin4I   HincII  HindIII     KasI     MfeI     MmeI     MslI     NarI
     NcoI     NdeI     NsiI     PfoI     PsiI     SalI     SfcI     SfoI
     SmlI     SpeI     SspI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI   Bpu10I     BsaI    BsaAI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I     ClaI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KpnI     MluI     MscI   MspA1I
     NaeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     StuI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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