Restriction map for pF1KB9960
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaHI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       BstAPI      BstBI       BstUI       
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoT14I     FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    StuI        StyI        SwaI        Tth111I     
XcmI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          871  GTATAC               GT^[AC][GT]AC
AflIII        512  ACATGT               A^C[GA][CT]GT
AlwNI          52  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         484  GAGCTC               G[AT]GC[AT]^C
BciVI         240  GGATAC               GTATCC(6/5)
BglII        1281  AGATCT               A^GATCT
BsaAI        1070  TACGTG               [CT]AC^GT[GA]
BsaBI        1351  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1148  GAGACC               GGTCTC(1/5)
BsaWI        1321  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       484  GAGCTC               G[AT]GC[AT]^C
BsmFI        1610  GGGAC                GGGAC(10/14)
Bsp1407I      833  TGTACA               T^GTACA
BspLU11I      512  ACATGT               A^CATGT
BsrGI         833  TGTACA               T^GTACA
Bst1107I      871  GTATAC               GTA^TAC
BstEII        595  GGTGACC              G^GT[ACGT]ACC
BstPI         595  GGTGACC              G^GT[ACGT]ACC
BstXI        1299  CCAAGTACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       871  GTATAC               GTA^TAC
EcoNI         126  CCTCTCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       595  GGTGACC              G^GT[ACGT]ACC
EcoRV        1660  GATATC               GAT^ATC
EcoT22I       884  ATGCAT               ATGCA^T
MaeII        1071  ACGT                 A^CGT
MamI         1351  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI         1263  CATATG               CA^TATG
NsiI          884  ATGCAT               ATGCA^T
NspI          512  ACATGT               [GA]CATG^[CT]
PflMI        1491  CCAAGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          857  GACTC                GAGTC(4/5)
PstI           99  CTGCAG               CTGCA^G
SacI          484  GAGCTC               GAGCT^C
ScaI          428  AGTACT               AGT^ACT
SfcI           99  CTGCAG               C^T[GA][CT]AG
SspBI         833  TGTACA               T^GTACA
SspI          377  AATATT               AAT^ATT
TaqI         1385  TCGA                 T^CGA
TthHB8I      1385  TCGA                 T^CGA
Van91I       1491  CCAAGCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1284  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AseI          361  ATTAAT               AT^TAAT
             1197  ATTAAT              
AsnI          361  ATTAAT               AT^TAAT
             1197  ATTAAT              
Asp700I       737  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1519  GAAGTGATTC          
BanII         484  GAGCTC               G[GA]GC[CT]^C
              604  GAGCCC              
BcnI          335  CCGGG                CC^[GC]GG
             1227  CCCGG               
BmyI          484  GAGCTC               G[AGT]GC[ACT]^C
              604  GAGCCC              
BpmI          949  CTGGAG               CTGGAG(16/14)
             1145  CTGGAG              
Bsp1286I      484  GAGCTC               G[AGT]GC[ACT]^C
              604  GAGCCC              
BstYI        1281  AGATCT               [GA]^GATC[CT]
             1589  AGATCC              
DraI         1155  TTTAAA               TTT^AAA
             1314  TTTAAA              
EarI          655  GAAGAG               CTCTTC(1/4)
             1585  GAAGAG              
FauI           69  GCGGG                CCCGC(4/6)
               73  GCGGG               
Ksp632I       655  GAAGAG               CTCTTC(1/4)
             1585  GAAGAG              
MflI         1281  AGATCT               [GA]^GATC[CT]
             1589  AGATCC              
MslI          246  CACAGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              862  CATGCTGATG          
NciI          335  CCGGG                CC^[GC]GG
             1227  CCCGG               
PpuMI        1223  AGGACCC              [GA]G^G[AT]CC[CT]
             1436  AGGACCT             
PshBI         361  ATTAAT               AT^TAAT
             1197  ATTAAT              
PvuII          96  CAGCTG               CAG^CTG
              248  CAGCTG              
XhoII        1281  AGATCT               [GA]^GATC[CT]
             1589  AGATCC              
XmnI          737  GAATCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1519  GAAGTGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BalI        BbvI        BclI        
BfaI        BsaJI       BsiYI       BslI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      FbaI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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