Restriction map for pF1KB9960
 (Linear) (Six-base) MAPSORT of: KIBB9960.seq  Check: 6659  from: 1  to: 2067



KIBB9960.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AccI GT'mk_AC

Cuts at:      0     872    2067 
   Size:        872    1195
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     490     979    1433    1615    1893    2067 
   Size:        196     294     489     454     182     278     174

  Fragments arranged by size: 

                 489     454     294     278     196     182     174
 
AflIII A'CryG_T

Cuts at:      0     512    2067 
   Size:        512    1555
 
AleI CACnn'nnGTG

Cuts at:      0     250    2067 
   Size:        250    1817
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     114    2067 
   Size:        114    1953
 
AlwNI CAG_nnn'CTG

Cuts at:      0      57    2067 
   Size:         57    2010
 
ApoI r'AATT_y

Cuts at:      0     922    1336    1532    2067 
   Size:        922     414     196     535

  Fragments arranged by size: 

                 922     535     414     196
 
AseI AT'TA_AT

Cuts at:      0     362    1198    2067 
   Size:        362     836     869

  Fragments arranged by size: 

                 869     836     362
 
BanII G_rGCy'C

Cuts at:      0     488     608    2067 
   Size:        488     120    1459

  Fragments arranged by size: 

                1459     488     120
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     234    2067 
   Size:        234    1833
 
BclI T'GATC_A

Cuts at:      0     519    1137    1350    2067 
   Size:        519     618     213     717

  Fragments arranged by size: 

                 717     618     519     213
 
BfrBI ATG'CAT

Cuts at:      0     886    2067 
   Size:        886    1181
 
BglII A'GATC_T

Cuts at:      0    1281    2067 
   Size:       1281     786
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1232    2067 
   Size:       1232     835
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     970    1166    2067 
   Size:        970     196     901

  Fragments arranged by size: 

                 970     901     196
 
Bpu10I CC'TnA_GC

Cuts at:      0    1703    2067 
   Size:       1703     364
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1142    2067 
   Size:       1142     925
 
BsaAI yAC'GTr

Cuts at:      0    1072    2067 
   Size:       1072     995
 
BsaBI GATnn'nnATC

Cuts at:      0    1355    2067 
   Size:       1355     712
 
BsaWI w'CCGG_w

Cuts at:      0    1321    2067 
   Size:       1321     746
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1472    1502    2067 
   Size:       1472      30     565

  Fragments arranged by size: 

                1472     565      30
 
BseYI C'CCAG_C

Cuts at:      0      27    1756    2067 
   Size:         27    1729     311

  Fragments arranged by size: 

                1729     311      27
 
BsiHKAI G_wGCw'C

Cuts at:      0     488    2067 
   Size:        488    1579
 
Bsp1286I G_dGCh'C

Cuts at:      0     488     608    2067 
   Size:        488     120    1459

  Fragments arranged by size: 

                1459     488     120
 
BsrGI T'GTAC_A

Cuts at:      0     833    2067 
   Size:        833    1234
 
BstEII G'GTnAC_C

Cuts at:      0     595    2067 
   Size:        595    1472
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1306    2067 
   Size:       1306     761
 
BstYI r'GATC_y

Cuts at:      0    1281    1589    2067 
   Size:       1281     308     478

  Fragments arranged by size: 

                1281     478     308
 
BstZ17I GTA'TAC

Cuts at:      0     873    2067 
   Size:        873    1194
 
BtsI GCAGTG_nn'

Cuts at:      0    1578    2040    2067 
   Size:       1578     462      27

  Fragments arranged by size: 

                1578     462      27
 
DraI TTT'AAA

Cuts at:      0    1157    1316    2067 
   Size:       1157     159     751

  Fragments arranged by size: 

                1157     751     159
 
EaeI y'GGCC_r

Cuts at:      0      48     347    1505    1711    2067 
   Size:         48     299    1158     206     356

  Fragments arranged by size: 

                1158     356     299     206      48
 
EarI CTCTTCn'nnn_

Cuts at:      0     650    1580    2067 
   Size:        650     930     487

  Fragments arranged by size: 

                 930     650     487
 
EcoICRI GAG'CTC

Cuts at:      0     486    2067 
   Size:        486    1581
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     490     970     979    1166    1433    1615    1893 
   Size:        196     294     480       9     187     267     182     278

Cuts at:   1893    2067 
   Size:        174

  Fragments arranged by size: 

                 480     294     278     267     196     187     182     174
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     130    2067 
   Size:        130    1937
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1224    1437    1929    2067 
   Size:       1224     213     492     138

  Fragments arranged by size: 

                1224     492     213     138
 
EcoRV GAT'ATC

Cuts at:      0    1662    2067 
   Size:       1662     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     658     690    1574    1606    1651    1683    2067 
   Size:        658      32     884      32      45      32     384

  Fragments arranged by size: 

                 884     658     384      45      32      32      32
 
MscI TGG'CCA

Cuts at:      0      50     349    1507    1713    2067 
   Size:         50     299    1158     206     354

  Fragments arranged by size: 

                1158     354     299     206      50
 
MslI CAynn'nnrTG

Cuts at:      0     250     866    2067 
   Size:        250     616    1201

  Fragments arranged by size: 

                1201     616     250
 
MspA1I CmG'CkG

Cuts at:      0      57      98     250    2067 
   Size:         57      41     152    1817

  Fragments arranged by size: 

                1817     152      57      41
 
NdeI CA'TA_TG

Cuts at:      0    1264    2067 
   Size:       1264     803
 
NsiI A_TGCA'T

Cuts at:      0     888    2067 
   Size:        888    1179
 
NspI r_CATG'y

Cuts at:      0     516    2067 
   Size:        516    1551
 
PciI A'CATG_T

Cuts at:      0     512    2067 
   Size:        512    1555
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1497    2067 
   Size:       1497     570
 
PpuMI rG'GwC_Cy

Cuts at:      0    1224    1437    2067 
   Size:       1224     213     630

  Fragments arranged by size: 

                1224     630     213
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0       2      34     392     424    2067 
   Size:          2      32     358      32    1643

  Fragments arranged by size: 

                1643     358      32      32       2
 
PstI C_TGCA'G

Cuts at:      0     103    2067 
   Size:        103    1964
 
PvuII CAG'CTG

Cuts at:      0      98     250    2067 
   Size:         98     152    1817

  Fragments arranged by size: 

                1817     152      98
 
SacI G_AGCT'C

Cuts at:      0     488    2067 
   Size:        488    1579
 
ScaI AGT'ACT

Cuts at:      0     430    2067 
   Size:        430    1637
 
SfcI C'TryA_G

Cuts at:      0      99    2067 
   Size:         99    1968
 
SspI AAT'ATT

Cuts at:      0     379    2067 
   Size:        379    1688
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1634    1951    2067 
   Size:       1634     317     116

  Fragments arranged by size: 

                1634     317     116
 
TatI w'GTAC_w

Cuts at:      0     428     833    1302    2067 
   Size:        428     405     469     765

  Fragments arranged by size: 

                 765     469     428     405
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     530     562     568     600    2067 
   Size:        530      32       6      32    1467

  Fragments arranged by size: 

                1467     530      32      32       6
 
XbaI T'CTAG_A

Cuts at:      0    1284    2067 
   Size:       1284     783
 
XmnI GAAnn'nnTTC

Cuts at:      0     741    1523    2067 
   Size:        741     782     544

  Fragments arranged by size: 

                 782     741     544
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI     AseI
    BanII    BciVI     BclI    BfrBI    BglII     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI  BsiHKAI Bsp1286I
    BsrGI   BstEII    BstXI    BstYI  BstZ17I     BtsI     DraI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV    Hin4I     MscI
     MslI   MspA1I     NdeI     NsiI     NspI     PciI    PflMI    PpuMI
     PsrI     PstI    PvuII     SacI     ScaI     SfcI     SspI    TaqII
     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI    BpuEI    BsaHI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI  Tth111I     XcmI     XhoI     XmaI     ZraI
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