Restriction map for pF1KB9937
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BcgI        BciVI       BfrI        
BglI        BglII       BlnI        BsaBI       BsaWI       
BseAI       BsgI        BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
Eam1105I    EcoO65I     EcoRI       EcoRV       FseI        
FspI        HpaI        MamI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NcoI        
NgoMIV      NotI        NruI        PacI        PflFI       
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        SexAI       SfiI        SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         778  GACGTC               GACGT^C
AlwNI        1513  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       609  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         1208  TGGCCA               TGG^CCA
BamHI         227  GGATCC               G^GATCC
BbeI          145  GGCGCC               GGCGC^C
BbsI         1242  GAAGAC               GAAGAC(2/6)
BbvCI        1111  GCTGAGG              CCTCAGC(-5/-2)
BclI         1478  TGATCA               T^GATCA
BlpI         1177  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1177  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1242  GAAGAC               GAAGAC(2/6)
BsaI          847  GGTCTC               GGTCTC(1/5)
BsiEI          83  CGGCCG               CG[GA][CT]^CG
BsmI          477  GAATGC               GAATGC(1/-1)
BsrFI         142  ACCGGC               [GA]^CCGG[CT]
BstBI         372  TTCGAA               TT^CGAA
CelII        1177  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        142  ACCGGC               [GA]^CCGG[CT]
DraII         770  AGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI          772  GACCCTGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           83  CGGCCG               C^GGCCG
EclXI          83  CGGCCG               C^GGCCG
Eco52I         83  CGGCCG               C^GGCCG
EcoNI        1542  CCTTCCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      770  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       479  ATGCAT               ATGCA^T
FauI         1290  GCGGG                CCCGC(4/6)
FbaI         1478  TGATCA               T^GATCA
HgaI            4  GCGTC                GACGC(5/10)
HincII       1000  GTCAAC               GT[CT]^[GA]AC
HindII       1000  GTCAAC               GT[CT]^[GA]AC
KasI          145  GGCGCC               G^GCGCC
KspI          149  CCGCGG               CCGC^GG
MluNI        1208  TGGCCA               TGG^CCA
MscI         1208  TGGCCA               TGG^CCA
NarI          145  GGCGCC               GG^CGCC
NdeI          482  CATATG               CA^TATG
NsiI          479  ATGCAT               ATGCA^T
NspI          978  GCATGC               [GA]CATG^[CT]
NspV          372  TTCGAA               TT^CGAA
PaeR7I       1360  CTCGAG               C^TCGAG
PpuMI         770  AGGACCC              [GA]G^G[AT]CC[CT]
SacII         149  CCGCGG               CCGC^GG
ScaI         1526  AGTACT               AGT^ACT
SfoI          145  GGCGCC               GGC^GCC
SfuI          372  TTCGAA               TT^CGAA
SgrAI         141  CACCGGCG             C[GA]^CCGG[CT]G
SphI          978  GCATGC               GCATG^C
XcmI          828  CCAGACATTGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1360  CTCGAG               C^TCGAG
XmnI          609  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

Acc65I        731  GGTACC               G^GTACC
              823  GGTACC              
AccII         150  CGCG                 CG^CG
             1219  CGCG                
AcsI          366  AAATTC               [GA]^AATT[CT]
              697  GAATTT              
AocI          352  CCTCAGG              CC^T[ACGT]AGG
             1546  CCTGAGG             
ApoI          366  AAATTC               [GA]^AATT[CT]
              697  GAATTT              
Asp718I       731  GGTACC               G^GTACC
              823  GGTACC              
AspHI        1065  GAGCTC               G[AT]GC[AT]^C
             1180  GAGCTC              
AvaII         771  GGACC                G^G[AT]CC
             1419  GGTCC               
BanII        1065  GAGCTC               G[GA]GC[CT]^C
             1180  GAGCTC              
BbrPI         812  CACGTG               CAC^GTG
             1474  CACGTG              
BpmI          937  CTCCAG               CTGGAG(16/14)
               61  CTGGAG              
BsaAI         812  CACGTG               [CT]AC^GT[GA]
             1474  CACGTG              
BsiHKAI      1065  GAGCTC               G[AT]GC[AT]^C
             1180  GAGCTC              
BsrI         1529  ACTGG                ACTGG(1/-1)
             1035  CCAGT               
BstUI         150  CGCG                 CG^CG
             1219  CGCG                
Bsu36I        352  CCTCAGG              CC^T[ACGT]AGG
             1546  CCTGAGG             
DsaI          149  CCGCGG               C^C[GA][CT]GG
              818  CCGTGG              
EaeI           83  CGGCCG               [CT]^GGCC[GA]
             1208  TGGCCA              
Eco81I        352  CCTCAGG              CC^T[ACGT]AGG
             1546  CCTGAGG             
HindIII       513  AAGCTT               A^AGCTT
              586  AAGCTT              
HphI         1377  GGTGA                GGTGA(8/7)
              140  TCACC               
KpnI          731  GGTACC               GGTAC^C
              823  GGTACC              
MslI          648  CATAGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              897  CATCAAGATG          
MvnI          150  CGCG                 CG^CG
             1219  CGCG                
NheI          324  GCTAGC               G^CTAGC
             1017  GCTAGC              
PmaCI         812  CACGTG               CAC^GTG
             1474  CACGTG              
PmlI          812  CACGTG               CAC^GTG
             1474  CACGTG              
PvuII        1105  CAGCTG               CAG^CTG
             1516  CAGCTG              
SacI         1065  GAGCTC               GAGCT^C
             1180  GAGCTC              
SapI          639  GAAGAGC              GCTCTTC(1/4)
              802  GAAGAGC             
TfiI          682  GATTC                G^A[AT]TC
              910  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
Aor51HI     AvaI        BanI        BbvI        BcnI        
BfaI        BmyI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EarI        Eco47III    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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