Restriction map for pF1KB6967
 (Linear) (Six-base) MAPSORT of: KIBB6967.seq  Check: 2368  from: 1  to: 645



KIBB6967.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AatII G_ACGT'C

Cuts at:      0     582     645 
   Size:        582      63
 
AlwNI CAG_nnn'CTG

Cuts at:      0      70     645 
   Size:         70     575
 
ApoI r'AATT_y

Cuts at:      0     383     645 
   Size:        383     262
 
AvaI C'yCGr_G

Cuts at:      0      16     645 
   Size:         16     629
 
BanI G'GyrC_C

Cuts at:      0      40     645 
   Size:         40     605
 
BanII G_rGCy'C

Cuts at:      0     266     285     645 
   Size:        266      19     360

  Fragments arranged by size: 

                 360     266      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      94     645 
   Size:         94     551
 
Bme1580I G_kGCm'C

Cuts at:      0      43      64     645 
   Size:         43      21     581

  Fragments arranged by size: 

                 581      43      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     140     645 
   Size:        140     505
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     467     645 
   Size:        467     178
 
BsaBI GATnn'nnATC

Cuts at:      0     471     563     645 
   Size:        471      92      82

  Fragments arranged by size: 

                 471      92      82
 
BsaHI Gr'CG_yC

Cuts at:      0     579     645 
   Size:        579      66
 
BsaWI w'CCGG_w

Cuts at:      0     224     645 
   Size:        224     421
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     479     645 
   Size:        479     166
 
BseYI C'CCAG_C

Cuts at:      0      70     645 
   Size:         70     575
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     644     645 
   Size:        644       1
 
BsiHKAI G_wGCw'C

Cuts at:      0     266     285     645 
   Size:        266      19     360

  Fragments arranged by size: 

                 360     266      19
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     570     624     645 
   Size:        570      54      21

  Fragments arranged by size: 

                 570      54      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      43      64     266     285     645 
   Size:         43      21     202      19     360

  Fragments arranged by size: 

                 360     202      43      21      19
 
BspEI T'CCGG_A

Cuts at:      0     224     645 
   Size:        224     421
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     378     645 
   Size:        378     267
 
BssSI C'ACGA_G

Cuts at:      0     528     645 
   Size:        528     117
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     181     456     645 
   Size:        181     275     189

  Fragments arranged by size: 

                 275     189     181
 
DraI TTT'AAA

Cuts at:      0     509     645 
   Size:        509     136
 
EaeI y'GGCC_r

Cuts at:      0     429     645 
   Size:        429     216
 
EarI CTCTTCn'nnn_

Cuts at:      0     401     585     645 
   Size:        401     184      60

  Fragments arranged by size: 

                 401     184      60
 
EcoICRI GAG'CTC

Cuts at:      0     264     283     645 
   Size:        264      19     362

  Fragments arranged by size: 

                 362     264      19
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27      54     645 
   Size:         27      27     591

  Fragments arranged by size: 

                 591      27      27
 
HincII GTy'rAC

Cuts at:      0      13     645 
   Size:         13     632
 
MscI TGG'CCA

Cuts at:      0     431     645 
   Size:        431     214
 
MslI CAynn'nnrTG

Cuts at:      0     236     645 
   Size:        236     409
 
MspA1I CmG'CkG

Cuts at:      0      94     291     297     400     645 
   Size:         94     197       6     103     245

  Fragments arranged by size: 

                 245     197     103      94       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      70     645 
   Size:         70     575
 
PfoI T'CCnGG_A

Cuts at:      0     571     645 
   Size:        571      74
 
PpuMI rG'GwC_Cy

Cuts at:      0      27      54     645 
   Size:         27      27     591

  Fragments arranged by size: 

                 591      27      27
 
PshAI GACnn'nnGTC

Cuts at:      0     192     645 
   Size:        192     453
 
PspXI vC'TCGA_Gb

Cuts at:      0      16     645 
   Size:         16     629
 
PstI C_TGCA'G

Cuts at:      0     290     296     324     645 
   Size:        290       6      28     321

  Fragments arranged by size: 

                 321     290      28       6
 
PvuII CAG'CTG

Cuts at:      0      94     291     400     645 
   Size:         94     197     109     245

  Fragments arranged by size: 

                 245     197     109      94
 
SacI G_AGCT'C

Cuts at:      0     266     285     645 
   Size:        266      19     360

  Fragments arranged by size: 

                 360     266      19
 
SanDI GG'GwC_CC

Cuts at:      0      27     645 
   Size:         27     618
 
SapI GCTCTTCn'nnn_

Cuts at:      0     585     645 
   Size:        585      60
 
SfcI C'TryA_G

Cuts at:      0     286     292     320     645 
   Size:        286       6      28     325

  Fragments arranged by size: 

                 325     286      28       6
 
SmlI C'TyrA_G

Cuts at:      0      16     645 
   Size:         16     629
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      58      62     645 
   Size:         58       4     583

  Fragments arranged by size: 

                 583      58       4
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     178     645 
   Size:        178     467
 
XhoI C'TCGA_G

Cuts at:      0      16     645 
   Size:         16     629
 
XmnI GAAnn'nnTTC

Cuts at:      0     384     645 
   Size:        384     261
 
ZraI GAC'GTC

Cuts at:      0     580     645 
   Size:        580      65
 

 Enzymes that do cut:

    AatII    AlwNI     ApoI     AvaI     BanI    BanII     BbsI Bme1580I
     BmrI     BplI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BssSI    BstXI     DraI
     EaeI     EarI  EcoICRI EcoO109I   HincII     MscI     MslI   MspA1I
    PflMI     PfoI    PpuMI    PshAI    PspXI     PstI    PvuII     SacI
    SanDI     SapI     SfcI     SmlI    TaqII     XcmI     XhoI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI    BsiEI    BsiWI
     BsmI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI     PsiI
   PspOMI     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XmaI
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