Restriction map for pF1KB9870
 (Linear) (Six-base) MAPSORT of: KIBB9870.seq  Check: 9232  from: 1  to: 1431



KIBB9870.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     218    1431 
   Size:        218    1213
 
AatII G_ACGT'C

Cuts at:      0     344    1028    1330    1431 
   Size:        344     684     302     101

  Fragments arranged by size: 

                 684     344     302     101
 
AleI CACnn'nnGTG

Cuts at:      0    1147    1431 
   Size:       1147     284
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     637     669    1431 
   Size:        637      32     762

  Fragments arranged by size: 

                 762     637      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60    1116    1431 
   Size:         60    1056     315

  Fragments arranged by size: 

                1056     315      60
 
ApaI G_GGCC'C

Cuts at:      0     113    1431 
   Size:        113    1318
 
AvaI C'yCGr_G

Cuts at:      0      33    1431 
   Size:         33    1398
 
BanI G'GyrC_C

Cuts at:      0      22      96    1431 
   Size:         22      74    1335

  Fragments arranged by size: 

                1335      74      22
 
BanII G_rGCy'C

Cuts at:      0      88     113     629    1178    1431 
   Size:         88      25     516     549     253

  Fragments arranged by size: 

                 549     516     253      88      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     225     903    1020    1194    1431 
   Size:        225     678     117     174     237

  Fragments arranged by size: 

                 678     237     225     174     117
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     619     653    1431 
   Size:        619      34     778

  Fragments arranged by size: 

                 778     619      34
 
BclI T'GATC_A

Cuts at:      0     899    1431 
   Size:        899     532
 
BglI GCCn_nnn'nGGC

Cuts at:      0     108     307    1431 
   Size:        108     199    1124

  Fragments arranged by size: 

                1124     199     108
 
BglII A'GATC_T

Cuts at:      0    1316    1431 
   Size:       1316     115
 
Bme1580I G_kGCm'C

Cuts at:      0      99     113    1431 
   Size:         99      14    1318

  Fragments arranged by size: 

                1318      99      14
 
BmgBI CAC'GTC

Cuts at:      0     633    1431 
   Size:        633     798
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     115     426    1431 
   Size:        115     311    1005

  Fragments arranged by size: 

                1005     311     115
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1197    1431 
   Size:       1197     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     411     639     805    1431 
   Size:        411     228     166     626

  Fragments arranged by size: 

                 626     411     228     166
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     868    1431 
   Size:        868     563
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     865    1006    1431 
   Size:        865     141     425

  Fragments arranged by size: 

                 865     425     141
 
BsaHI Gr'CG_yC

Cuts at:      0     341     601    1025    1327    1431 
   Size:        341     260     424     302     104

  Fragments arranged by size: 

                 424     341     302     260     104
 
BsaWI w'CCGG_w

Cuts at:      0     522    1431 
   Size:        522     909
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     637     667    1120    1150    1431 
   Size:        637      30     453      30     281

  Fragments arranged by size: 

                 637     453     281      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     625    1044    1300    1303    1431 
   Size:        625     419     256       3     128

  Fragments arranged by size: 

                 625     419     256     128       3
 
BseYI C'CCAG_C

Cuts at:      0     165    1043    1109    1116    1431 
   Size:        165     878      66       7     315

  Fragments arranged by size: 

                 878     315     165      66       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781    1143    1431 
   Size:        781     362     288

  Fragments arranged by size: 

                 781     362     288
 
BsiEI CG_ry'CG

Cuts at:      0     992    1305    1431 
   Size:        992     313     126

  Fragments arranged by size: 

                 992     313     126
 
BsiHKAI G_wGCw'C

Cuts at:      0     370     803    1178    1431 
   Size:        370     433     375     253

  Fragments arranged by size: 

                 433     375     370     253
 
BsmI GAATG_Cn'

Cuts at:      0     642    1431 
   Size:        642     789
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1147    1431 
   Size:       1147     284
 
Bsp1286I G_dGCh'C

Cuts at:      0      88      99     113     370     629     803    1178    1431 
   Size:         88      11      14     257     259     174     375     253

  Fragments arranged by size: 

                 375     259     257     253     174      88      14      11
            
 
BspHI T'CATG_A

Cuts at:      0     719    1431 
   Size:        719     712
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     218     584    1431 
   Size:        218     366     847

  Fragments arranged by size: 

                 847     366     218
 
BsrDI GCAATG_nn'

Cuts at:      0    1176    1431 
   Size:       1176     255
 
BsrFI r'CCGG_y

Cuts at:      0     310     991    1146    1431 
   Size:        310     681     155     285

  Fragments arranged by size: 

                 681     310     285     155
 
BssHII G'CGCG_C

Cuts at:      0     213     833     852    1431 
   Size:        213     620      19     579

  Fragments arranged by size: 

                 620     579     213      19
 
BstEII G'GTnAC_C

Cuts at:      0     123    1339    1431 
   Size:        123    1216      92

  Fragments arranged by size: 

                1216     123      92
 
BstYI r'GATC_y

Cuts at:      0     764    1316    1431 
   Size:        764     552     115

  Fragments arranged by size: 

                 764     552     115
 
BtgI C'CryG_G

Cuts at:      0      77    1431 
   Size:         77    1354
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     735    1431 
   Size:        735     696
 
BtsI GCAGTG_nn'

Cuts at:      0     371     972    1431 
   Size:        371     601     459

  Fragments arranged by size: 

                 601     459     371
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1030    1431 
   Size:       1030     401
 
EaeI y'GGCC_r

Cuts at:      0     248     308     590     989    1431 
   Size:        248      60     282     399     442

  Fragments arranged by size: 

                 442     399     282     248      60
 
EagI C'GGCC_G

Cuts at:      0     989    1431 
   Size:        989     442
 
EarI CTCTTCn'nnn_

Cuts at:      0     687    1431 
   Size:        687     744
 
EcoICRI GAG'CTC

Cuts at:      0    1176    1431 
   Size:       1176     255
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     411     639     805    1431 
   Size:        411     228     166     626

  Fragments arranged by size: 

                 626     411     228     166
 
EcoO109I rG'GnC_Cy

Cuts at:      0     109     110     431     982    1294    1431 
   Size:        109       1     321     551     312     137

  Fragments arranged by size: 

                 551     321     312     137     109       1
 
FspI TGC'GCA

Cuts at:      0     186    1431 
   Size:        186    1245
 
HaeII r_GCGC'y

Cuts at:      0     274    1431 
   Size:        274    1157
 
MscI TGG'CCA

Cuts at:      0     592    1431 
   Size:        592     839
 
MslI CAynn'nnrTG

Cuts at:      0     403     724    1147    1234    1431 
   Size:        403     321     423      87     197

  Fragments arranged by size: 

                 423     403     321     197      87
 
MspA1I CmG'CkG

Cuts at:      0      60    1113    1222    1431 
   Size:         60    1053     109     209

  Fragments arranged by size: 

                1053     209     109      60
 
NaeI GCC'GGC

Cuts at:      0     993    1431 
   Size:        993     438
 
NgoMIV G'CCGG_C

Cuts at:      0     991    1431 
   Size:        991     440
 
NspI r_CATG'y

Cuts at:      0    1311    1431 
   Size:       1311     120
 
PasI CC'CwG_GG

Cuts at:      0     605    1431 
   Size:        605     826
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      60    1116    1431 
   Size:         60    1056     315

  Fragments arranged by size: 

                1056     315      60
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     637     669    1431 
   Size:        637      32     762

  Fragments arranged by size: 

                 762     637      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1294    1431 
   Size:       1294     137
 
PshAI GACnn'nnGTC

Cuts at:      0     229    1431 
   Size:        229    1202
 
PspOMI G'GGCC_C

Cuts at:      0     109    1431 
   Size:        109    1322
 
PstI C_TGCA'G

Cuts at:      0     967    1431 
   Size:        967     464
 
PvuI CG_AT'CG

Cuts at:      0    1305    1431 
   Size:       1305     126
 
PvuII CAG'CTG

Cuts at:      0      60    1113    1222    1431 
   Size:         60    1053     109     209

  Fragments arranged by size: 

                1053     209     109      60
 
SacI G_AGCT'C

Cuts at:      0    1178    1431 
   Size:       1178     253
 
SfcI C'TryA_G

Cuts at:      0     963    1431 
   Size:        963     468
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1146    1431 
   Size:       1146     285
 
SmaI CCC'GGG

Cuts at:      0      35    1431 
   Size:         35    1396
 
SmlI C'TyrA_G

Cuts at:      0     883    1431 
   Size:        883     548
 
StyI C'CwwG_G

Cuts at:      0     470     875    1431 
   Size:        470     405     556

  Fragments arranged by size: 

                 556     470     405
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     336    1431 
   Size:        336    1095
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1120    1152    1431 
   Size:       1120      32     279

  Fragments arranged by size: 

                1120     279      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     498     891    1198    1431 
   Size:        498     393     307     233

  Fragments arranged by size: 

                 498     393     307     233
 
XmaI C'CCGG_G

Cuts at:      0      33    1431 
   Size:         33    1398
 
XmnI GAAnn'nnTTC

Cuts at:      0    1423    1431 
   Size:       1423       8
 
ZraI GAC'GTC

Cuts at:      0     342    1026    1328    1431 
   Size:        342     684     302     103

  Fragments arranged by size: 

                 684     342     302     103
 

 Enzymes that do cut:

     AarI    AatII     AleI     AloI    AlwNI     ApaI     AvaI     BanI
    BanII     BbsI     BcgI     BclI     BglI    BglII Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrDI    BsrFI   BssHII   BstEII    BstYI     BtgI
    BtgZI     BtsI     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
 EcoO109I     FspI    HaeII     MscI     MslI   MspA1I     NaeI   NgoMIV
     NspI     PasI    PflMI     PpiI    PpuMI    PshAI   PspOMI     PstI
     PvuI    PvuII     SacI     SfcI    SgrAI     SmaI     SmlI     StyI
    TaqII     TstI  Tth111I     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI    BfrBI     BlpI     BmtI
   Bpu10I    BsaAI    BsaBI    BsiWI    BspEI    BsrBI    BsrGI    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PfoI     PmeI     PmlI     PsiI    PspXI     PsrI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     XbaI     XcmI     XhoI
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