Restriction map for pF1KB6926
 (Linear) (Six-base) MAPSORT of: KIBB6926.seq  Check: 3580  from: 1  to: 684



KIBB6926.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     684 
   Size:        198     486
 
AfeI AGC'GCT

Cuts at:      0     661     684 
   Size:        661      23
 
AflIII A'CryG_T

Cuts at:      0     142     684 
   Size:        142     542
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     127     684 
   Size:        127     557
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     383     671     684 
   Size:         75     308     288      13

  Fragments arranged by size: 

                 308     288      75      13
 
BanII G_rGCy'C

Cuts at:      0     620     684 
   Size:        620      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     488     684 
   Size:        488     196
 
BclI T'GATC_A

Cuts at:      0     425     684 
   Size:        425     259
 
BglI GCCn_nnn'nGGC

Cuts at:      0     568     684 
   Size:        568     116
 
BglII A'GATC_T

Cuts at:      0     260     684 
   Size:        260     424
 
Bme1580I G_kGCm'C

Cuts at:      0      92     684 
   Size:         92     592
 
BmgBI CAC'GTC

Cuts at:      0     420     684 
   Size:        420     264
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544     648     684 
   Size:        544     104      36

  Fragments arranged by size: 

                 544     104      36
 
Bpu10I CC'TnA_GC

Cuts at:      0      43     323     684 
   Size:         43     280     361

  Fragments arranged by size: 

                 361     280      43
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     484     520     684 
   Size:        484      36     164

  Fragments arranged by size: 

                 484     164      36
 
BsaHI Gr'CG_yC

Cuts at:      0     131     684 
   Size:        131     553
 
BsaWI w'CCGG_w

Cuts at:      0     181     684 
   Size:        181     503
 
BseYI C'CCAG_C

Cuts at:      0     274     619     684 
   Size:        274     345      65

  Fragments arranged by size: 

                 345     274      65
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     620     684 
   Size:         92     528      64

  Fragments arranged by size: 

                 528      92      64
 
BspEI T'CCGG_A

Cuts at:      0     181     684 
   Size:        181     503
 
BspHI T'CATG_A

Cuts at:      0     399     684 
   Size:        399     285
 
BsrFI r'CCGG_y

Cuts at:      0      33     684 
   Size:         33     651
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      75     684 
   Size:         75     609
 
BstYI r'GATC_y

Cuts at:      0     260     629     684 
   Size:        260     369      55

  Fragments arranged by size: 

                 369     260      55
 
Bsu36I CC'TnA_GG

Cuts at:      0      93     684 
   Size:         93     591
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     558     684 
   Size:        558     126
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     130     684 
   Size:        130     554
 
EaeI y'GGCC_r

Cuts at:      0     497     569     684 
   Size:        497      72     115

  Fragments arranged by size: 

                 497     115      72
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     463     625     684 
   Size:        463     162      59

  Fragments arranged by size: 

                 463     162      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     544     648     684 
   Size:        198     346     104      36

  Fragments arranged by size: 

                 346     198     104      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     498     684 
   Size:        498     186
 
EcoO109I rG'GnC_Cy

Cuts at:      0     170     380     684 
   Size:        170     210     304

  Fragments arranged by size: 

                 304     210     170
 
HaeII r_GCGC'y

Cuts at:      0     536     663     684 
   Size:        536     127      21

  Fragments arranged by size: 

                 536     127      21
 
MscI TGG'CCA

Cuts at:      0     499     571     684 
   Size:        499      72     113

  Fragments arranged by size: 

                 499     113      72
 
MslI CAynn'nnrTG

Cuts at:      0     404     550     684 
   Size:        404     146     134

  Fragments arranged by size: 

                 404     146     134
 
MspA1I CmG'CkG

Cuts at:      0     274     668     684 
   Size:        274     394      16

  Fragments arranged by size: 

                 394     274      16
 
NspI r_CATG'y

Cuts at:      0     146     684 
   Size:        146     538
 
PciI A'CATG_T

Cuts at:      0     142     684 
   Size:        142     542
 
PfoI T'CCnGG_A

Cuts at:      0     521     684 
   Size:        521     163
 
PstI C_TGCA'G

Cuts at:      0     260     684 
   Size:        260     424
 
PvuII CAG'CTG

Cuts at:      0     668     684 
   Size:        668      16
 
SfcI C'TryA_G

Cuts at:      0     256     684 
   Size:        256     428
 
StuI AGG'CCT

Cuts at:      0     328     684 
   Size:        328     356
 
StyI C'CwwG_G

Cuts at:      0     494     578     684 
   Size:        494      84     106

  Fragments arranged by size: 

                 494     106      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316     684 
   Size:        316     368
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AlfI    AlwNI    BanII     BbsI     BclI
     BglI    BglII Bme1580I    BmgBI     BpmI   Bpu10I     BsaI    BsaHI
    BsaWI    BseYI Bsp1286I    BspEI    BspHI    BsrFI   BstAPI    BstYI
   Bsu36I    BtgZI     DrdI     EaeI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII     MscI     MslI   MspA1I     NspI     PciI     PfoI     PstI
    PvuII     SfcI     StuI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BlpI     BmrI     BmtI     BplI    BpuEI    BsaAI
    BsaBI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com