Restriction map for pF1KB6921
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BanII       BbrPI       BbvCI       BcgI        BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     MaeI        MaeII       
MaeIII      MfeI        MluI        MluNI       MroI        
MscI        MunI        NciI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccII          50  CGCG                 CG^CG
AlwNI          69  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       525  GAACGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         220  GGACC                G^G[AT]CC
BamHI         543  GGATCC               G^GATCC
BanI          562  GGCGCC               G^G[CT][GA]CC
BbeI          562  GGCGCC               GGCGC^C
BbsI          157  GTCTTC               GAAGAC(2/6)
BciVI         132  GTATCC               GTATCC(6/5)
BpuAI         157  GTCTTC               GAAGAC(2/6)
BsaBI         183  GATACCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          207  GAGACC               GGTCTC(1/5)
BsgI          401  GTGCAG               GTGCAG(16/14)
BsmFI          53  GGGAC                GGGAC(10/14)
BsrFI          36  GCCGGC               [GA]^CCGG[CT]
BssKI         211  CCTGG                ^CC[ACGT]GG
BstF5I        556  GGATG                GGATG(2/0)
BstNI         211  CCTGG                CC^[AT]GG
BstUI          50  CGCG                 CG^CG
BstYI         543  GGATCC               [GA]^GATC[CT]
CfoI          563  GCGC                 GCG^C
Cfr10I         36  GCCGGC               [GA]^CCGG[CT]
DrdI           55  GACGCCTGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           49  CCGCGG               C^C[GA][CT]GG
EaeI           38  CGGCCA               [CT]^GGCC[GA]
EcoRII        211  CCTGG                ^CC[AT]GG
EcoT14I       548  CCAAGG               C^C[AT][AT]GG
FokI          556  GGATG                GGATG(9/13)
HaeII         562  GGCGCC               [GA]GCGC^[CT]
HapII          37  CCGG                 C^CGG
HhaI          563  GCGC                 GCG^C
HinP1I        563  GCGC                 G^CGC
HpaII          37  CCGG                 C^CGG
HphI          366  TCACC                GGTGA(8/7)
KasI          562  GGCGCC               G^GCGCC
KspI           49  CCGCGG               CCGC^GG
MamI          183  GATACCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          543  GGATCC               [GA]^GATC[CT]
MseI          347  TTAA                 T^TAA
MslI          534  CATCAGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           37  CCGG                 C^CGG
MvaI          211  CCTGG                CC^[AT]GG
MvnI           50  CGCG                 CG^CG
NaeI           36  GCCGGC               GCC^GGC
NarI          562  GGCGCC               GG^CGCC
NgoMIV         36  GCCGGC               G^CCGGC
PleI          451  GACTC                GAGTC(4/5)
PvuII          72  CAGCTG               CAG^CTG
SacII          49  CCGCGG               CCGC^GG
ScrFI         211  CCTGG                CC^[ACGT]GG
SfaNI         340  GATGC                GCATC(5/9)
SfcI           66  CTACAG               C^T[GA][CT]AG
SfoI          562  GGCGCC               GGC^GCC
SspI           91  AATATT               AAT^ATT
StyI          548  CCAAGG               C^C[AT][AT]GG
TaqI          293  TCGA                 T^CGA
Tru9I         347  TTAA                 T^TAA
TspRI         456  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       293  TCGA                 T^CGA
XhoII         543  GGATCC               [GA]^GATC[CT]
XmnI          525  GAACGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI           64  GTCTAC               GT^[AC][GT]AC
              568  GTCTAC              
AcsI          385  AAATTT               [GA]^AATT[CT]
              583  GAATTT              
AcyI           55  GACGCC               G[GA]^CG[CT]C
              562  GGCGCC              
AluI           73  AGCT                 AG^CT
              101  AGCT                
ApoI          385  AAATTT               [GA]^AATT[CT]
              583  GAATTT              
BsaHI          55  GACGCC               G[GA]^CG[CT]C
              562  GGCGCC              
BsaJI          49  CCGCGG               C^C[ACGT][ACGT]GG
              548  CCAAGG              
BsmAI         207  GAGAC                GTCTC(1/5)
              499  GAGAC               
Cfr13I         13  GGCCC                G^G[ACGT]CC
              220  GGACC               
HaeIII         13  GGCC                 GG^CC
               39  GGCC                
HgaI           55  GACGC                GACGC(5/10)
               62  GCGTC               
Hin1I          55  GACGCC               G[GA]^CG[CT]C
              562  GGCGCC              
MwoI           62  GCGTCTACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              474  GCCTCCGCCGC         
Sau96I         13  GGCCC                G^G[ACGT]CC
              220  GGACC               
TfiI          406  GATTC                G^A[AT]TC
              508  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AlwI        BbvI        BpmI        BsiYI       
BslI        BsrI        Cac8I       DpnII       FauI        
Fnu4HI      HinfI       ItaI        MboI        MboII       
MnlI        MspA1I      NdeII       NlaIII      NlaIV       
Sau3AI      TseI        Tsp509I     
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