Restriction map for pF1KE0850
 (Linear) (Six-base) MAPSORT of: KIEE0850.seq  Check: 398  from: 1  to: 951



KIEE0850.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746     951 
   Size:        746     205
 
ApaLI G'TGCA_C

Cuts at:      0     658     951 
   Size:        658     293
 
ApoI r'AATT_y

Cuts at:      0      28     951 
   Size:         28     923
 
AvaI C'yCGr_G

Cuts at:      0     478     951 
   Size:        478     473
 
BanII G_rGCy'C

Cuts at:      0      58     503     951 
   Size:         58     445     448

  Fragments arranged by size: 

                 448     445      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     306     715     951 
   Size:        306     409     236

  Fragments arranged by size: 

                 409     306     236
 
BbvCI CC'TCA_GC

Cuts at:      0     679     951 
   Size:        679     272
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     459     493     951 
   Size:        459      34     458

  Fragments arranged by size: 

                 459     458      34
 
BclI T'GATC_A

Cuts at:      0     461     951 
   Size:        461     490
 
Bme1580I G_kGCm'C

Cuts at:      0     662     915     951 
   Size:        662     253      36

  Fragments arranged by size: 

                 662     253      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      94     951 
   Size:         94     857
 
Bpu10I CC'TnA_GC

Cuts at:      0       4     679     951 
   Size:          4     675     272

  Fragments arranged by size: 

                 675     272       4
 
BsaBI GATnn'nnATC

Cuts at:      0     247     951 
   Size:        247     704
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     262     292     951 
   Size:        262      30     659

  Fragments arranged by size: 

                 659     262      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     681     684     951 
   Size:        681       3     267

  Fragments arranged by size: 

                 681     267       3
 
BseYI C'CCAG_C

Cuts at:      0     935     951 
   Size:        935      16
 
BsiHKAI G_wGCw'C

Cuts at:      0      58     641     662     951 
   Size:         58     583      21     289

  Fragments arranged by size: 

                 583     289      58      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     503     641     662     915     951 
   Size:         58     445     138      21     253      36

  Fragments arranged by size: 

                 445     253     138      58      36      21
 
BsrDI GCAATG_nn'

Cuts at:      0      73     951 
   Size:         73     878
 
BsrGI T'GTAC_A

Cuts at:      0     833     951 
   Size:        833     118
 
BssSI C'ACGA_G

Cuts at:      0      37      68     256     951 
   Size:         37      31     188     695

  Fragments arranged by size: 

                 695     188      37      31
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     601     951 
   Size:        601     350
 
Bsu36I CC'TnA_GG

Cuts at:      0     696     951 
   Size:        696     255
 
BtgI C'CryG_G

Cuts at:      0     113     632     902     951 
   Size:        113     519     270      49

  Fragments arranged by size: 

                 519     270     113      49
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     227     262     951 
   Size:        227      35     689

  Fragments arranged by size: 

                 689     227      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     258     951 
   Size:        258     693
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     274     951 
   Size:        274     677
 
EaeI y'GGCC_r

Cuts at:      0     116     192     329     668     951 
   Size:        116      76     137     339     283

  Fragments arranged by size: 

                 339     283     137     116      76
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     951 
   Size:         94     857
 
EcoICRI GAG'CTC

Cuts at:      0      56     951 
   Size:         56     895
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746     951 
   Size:        746     205
 
EcoO109I rG'GnC_Cy

Cuts at:      0     543     573     951 
   Size:        543      30     378

  Fragments arranged by size: 

                 543     378      30
 
HindIII A'AGCT_T

Cuts at:      0     528     951 
   Size:        528     423
 
MscI TGG'CCA

Cuts at:      0     118     670     951 
   Size:        118     552     281

  Fragments arranged by size: 

                 552     281     118
 
MslI CAynn'nnrTG

Cuts at:      0     118     951 
   Size:        118     833
 
NcoI C'CATG_G

Cuts at:      0     113     632     902     951 
   Size:        113     519     270      49

  Fragments arranged by size: 

                 519     270     113      49
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     258     951 
   Size:        258     693
 
PfoI T'CCnGG_A

Cuts at:      0     148     206     951 
   Size:        148      58     745

  Fragments arranged by size: 

                 745     148      58
 
PpuMI rG'GwC_Cy

Cuts at:      0     573     951 
   Size:        573     378
 
PshAI GACnn'nnGTC

Cuts at:      0     742     951 
   Size:        742     209
 
SacI G_AGCT'C

Cuts at:      0      58     951 
   Size:         58     893
 
SanDI GG'GwC_CC

Cuts at:      0     573     951 
   Size:        573     378
 
SfcI C'TryA_G

Cuts at:      0     102     951 
   Size:        102     849
 
SmlI C'TyrA_G

Cuts at:      0     478     951 
   Size:        478     473
 
StyI C'CwwG_G

Cuts at:      0     113     632     902     951 
   Size:        113     519     270      49

  Fragments arranged by size: 

                 519     270     113      49
 
TatI w'GTAC_w

Cuts at:      0     179     305     833     951 
   Size:        179     126     528     118

  Fragments arranged by size: 

                 528     179     126     118
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     262     294     951 
   Size:        262      32     657

  Fragments arranged by size: 

                 657     262      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     796     951 
   Size:        796     155
 
XhoI C'TCGA_G

Cuts at:      0     478     951 
   Size:        478     473
 
XmnI GAAnn'nnTTC

Cuts at:      0     712     951 
   Size:        712     239
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI     AvaI    BanII     BbsI    BbvCI     BcgI
     BclI Bme1580I     BplI   Bpu10I    BsaBI    BsaXI    BseRI    BseYI
  BsiHKAI Bsp1286I    BsrDI    BsrGI    BssSI   BstAPI   Bsu36I     BtgI
    CspCI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
  HindIII     MscI     MslI     NcoI    PflMI     PfoI    PpuMI    PshAI
     SacI    SanDI     SfcI     SmlI     StyI     TatI     TstI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BciVI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI     ClaI
     DraI     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XmaI     ZraI
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