Restriction map for pF1KB6902
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BbrPI       BbsI        BcgI        BciVI       
BfaI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        FspI        HgaI        HincII      HindII      
HindIII     HinfI       HpaI        HphI        KpnI        
Ksp632I     KspI        MaeI        MamI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        NcoI        NheI        
NotI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII          64  GACGTC               GACGT^C
AfaI          207  GTAC                 GT^AC
AlwI          576  GATCC                GGATC(4/5)
ApaI          390  GGGCCC               GGGCC^C
AvaI          326  CCCGGG               C^[CT]CG[GA]G
AvaII         201  GGACC                G^G[AT]CC
BanI           46  GGCGCC               G^G[CT][GA]CC
BanII         390  GGGCCC               G[GA]GC[CT]^C
BbeI           46  GGCGCC               GGCGC^C
BbvCI         337  GCTGAGG              CCTCAGC(-5/-2)
BclI          252  TGATCA               T^GATCA
BglI          137  GCCAAAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          390  GGGCCC               G[AGT]GC[ACT]^C
BsmFI         329  GGGAC                GGGAC(10/14)
BsoBI         326  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      390  GGGCCC               G[AGT]GC[ACT]^C
BspMI         273  GCAGGT               ACCTGC(4/8)
BsrBI          24  GAGCGG               CCGCTC(-3/-3)
BsrFI          43  GCCGGC               [GA]^CCGG[CT]
BstAPI        291  GCACCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         613  CCAACACGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         43  GCCGGC               [GA]^CCGG[CT]
DsaI          397  CCACGG               C^C[GA][CT]GG
FauI          451  GCGGG                CCCGC(4/6)
FbaI          252  TGATCA               T^GATCA
HaeII          46  GGCGCC               [GA]GCGC^[CT]
KasI           46  GGCGCC               G^GCGCC
MslI          147  CATTGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI           43  GCCGGC               GCC^GGC
NarI           46  GGCGCC               GG^CGCC
NdeI          237  CATATG               CA^TATG
NgoMIV         43  GCCGGC               G^CCGGC
NruI          306  TCGCGA               TCG^CGA
PstI          244  CTGCAG               CTGCA^G
PvuII         295  CAGCTG               CAG^CTG
RsaI          207  GTAC                 GT^AC
SfcI          244  CTGCAG               C^T[GA][CT]AG
SfoI           46  GGCGCC               GGC^GCC
SmaI          326  CCCGGG               CCC^GGG
XcmI          138  CCAAAAAGGCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         326  CCCGGG               C^CCGGG
XmaI          326  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AccII         307  CGCG                 CG^CG
              450  CGCG                
AcyI           46  GGCGCC               G[GA]^CG[CT]C
               64  GACGTC              
AlwNI          70  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              511  CAGTCACTG           
BsaHI          46  GGCGCC               G[GA]^CG[CT]C
               64  GACGTC              
BsrI          369  ACTGG                ACTGG(1/-1)
              510  CCAGT               
BstF5I        152  GGATG                GGATG(2/0)
              465  GGATG               
BstUI         307  CGCG                 CG^CG
              450  CGCG                
DdeI          338  CTGAG                C^T[ACGT]AG
              601  CTTAG               
FokI          152  GGATG                GGATG(9/13)
              465  GGATG               
Hin1I          46  GGCGCC               G[GA]^CG[CT]C
               64  GACGTC              
MspA1I        163  CCGCTG               C[AC]G^C[GT]G
              295  CAGCTG              
MvnI          307  CGCG                 CG^CG
              450  CGCG                
NlaIII        333  CATG                 CATG^
              357  CATG                


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DpnII       
DraII       EcoO109I    EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MaeII       MaeIII      MboI        MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp45I      TspRI       
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