Restriction map for pF1KB6859
##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaI        AviII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstUI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MboI        MfeI        MflI        
MluI        MroI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AciI          185  GCGG                 CCGC(-3/-1)
AluI          357  AGCT                 AG^CT
AlwNI         149  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         255  GTGCTC               G[AT]GC[AT]^C
AspI          508  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         490  GGACC                G^G[AT]CC
AvrII         474  CCTAGG               C^CTAGG
BalI          333  TGGCCA               TGG^CCA
BfaI          475  CTAG                 C^TAG
BlnI          474  CCTAGG               C^CTAGG
BmyI          255  GTGCTC               G[AGT]GC[ACT]^C
BpmI           90  CTGGAG               CTGGAG(16/14)
BsiHKAI       255  GTGCTC               G[AT]GC[AT]^C
BsiYI         336  CCAGCCCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          336  CCAGCCCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         179  GAGAC                GTCTC(1/5)
BsmFI         408  GGGAC                GGGAC(10/14)
Bsp1286I      255  GTGCTC               G[AGT]GC[ACT]^C
BstBI         212  TTCGAA               TT^CGAA
BstEII        520  GGTCACC              G^GT[ACGT]ACC
BstPI         520  GGTCACC              G^GT[ACGT]ACC
DdeI          446  CTCAG                C^T[ACGT]AG
EaeI          333  TGGCCA               [CT]^GGCC[GA]
EcoO65I       520  GGTCACC              G^GT[ACGT]ACC
FauI          185  GCGGG                CCCGC(4/6)
HphI          522  TCACC                GGTGA(8/7)
MaeI          475  CTAG                 C^TAG
MaeII         441  ACGT                 A^CGT
MluNI         333  TGGCCA               TGG^CCA
MnlI            5  CCTC                 CCTC(7/6)
MscI          333  TGGCCA               TGG^CCA
MspA1I        183  CAGCGG               C[AC]G^C[GT]G
NlaIV          92  GGAGCC               GG[ACGT]^[ACGT]CC
NspI           77  GCATGT               [GA]CATG^[CT]
NspV          212  TTCGAA               TT^CGAA
PflFI         508  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SfuI          212  TTCGAA               TT^CGAA
SspI           26  AATATT               AAT^ATT
Tth111I       508  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BbvI          247  GCTGC                GCAGC(8/12)
              265  GCTGC               
BstF5I         37  CATCC                GGATG(2/0)
              113  GGATG               
BstXI           8  CCAAATCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              341  CCAAGGTGGTGG        
Cac8I         166  GCAAGC               GC[ACGT]^[ACGT]GC
              335  GCCAGC              
Fnu4HI        247  GCTGC                GC^[ACGT]GC
              265  GCTGC               
FokI           37  CATCC                GGATG(9/13)
              113  GGATG               
HinfI         201  GATTC                G^A[ACGT]TC
              288  GATTC               
ItaI          247  GCTGC                GC^[ACGT]GC
              265  GCTGC               
MaeIII        514  GTCAC                ^GT[ACGT]AC
              521  GTCAC               
MboII         296  GAAGA                GAAGA(8/7)
              367  GAAGA               
MseI          223  TTAA                 T^TAA
              424  TTAA                
TaqI           99  TCGA                 T^CGA
              213  TCGA                
TfiI          201  GATTC                G^A[AT]TC
              288  GATTC               
Tru9I         223  TTAA                 T^TAA
              424  TTAA                
TseI          247  GCTGC                G^C[AT]GC
              265  GCTGC               
Tsp45I        514  GTCAC                ^GT[GC]AC
              521  GTCAC               
Tsp509I       426  AATT                 ^AATT
              504  AATT                
TspRI         253  CAGTG                CA[GC]TG[ACGT][ACGT]^
              516  CACTG               
TthHB8I        99  TCGA                 T^CGA
              213  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AfaI        BsaJI       BsrI        BssKI       BstNI       
Cfr13I      EcoRII      EcoT14I     HaeIII      MvaI        
MwoI        NlaIII      RsaI        Sau96I      ScrFI       
SfaNI       StyI        
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