Restriction map for pF1KB6881
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MfeI        MluI        MroI        MseI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tsp509I     
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          274  GTCGAC               GT^[AC][GT]AC
AlwI          384  GGATC                GGATC(4/5)
AlwNI         382  CAGGATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         343  GAGCTC               G[AT]GC[AT]^C
AvaI          308  CCCGGG               C^[CT]CG[GA]G
AviII          53  TGCGCA               TGC^GCA
BanII         343  GAGCTC               G[GA]GC[CT]^C
BbvI          406  GCTGC                GCAGC(8/12)
BcgI          435  GCATTCCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          343  GAGCTC               G[AGT]GC[ACT]^C
BpmI          231  CTGGAG               CTGGAG(16/14)
BsaBI          51  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       343  GAGCTC               G[AT]GC[AT]^C
BsmFI         488  GGGAC                GGGAC(10/14)
BsoBI         308  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      343  GAGCTC               G[AGT]GC[ACT]^C
BsrDI         281  GCAATG               GCAATG(2/0)
BstYI         384  GGATCT               [GA]^GATC[CT]
CfoI           54  GCGC                 GCG^C
DdeI          410  CTGAG                C^T[ACGT]AG
EarI          313  GAAGAG               CTCTTC(1/4)
EcoT14I       255  CCAAGG               C^C[AT][AT]GG
FauI          485  GCGGG                CCCGC(4/6)
FspI           53  TGCGCA               TGC^GCA
HhaI           54  GCGC                 GCG^C
HinP1I         54  GCGC                 G^CGC
HincII        274  GTCGAC               GT[CT]^[GA]AC
HindII        274  GTCGAC               GT[CT]^[GA]AC
HinfI         219  GATTC                G^A[ACGT]TC
Ksp632I       313  GAAGAG               CTCTTC(1/4)
MamI           51  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          384  GGATCT               [GA]^GATC[CT]
MslI           57  CATCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        483  CAGCGG               C[AC]G^C[GT]G
SacI          343  GAGCTC               GAGCT^C
SalI          274  GTCGAC               G^TCGAC
SapI          313  GAAGAGC              GCTCTTC(1/4)
SmaI          308  CCCGGG               CCC^GGG
SmlI           25  CTCAAG               C^T[CT][GA]AG
StuI          504  AGGCCT               AGG^CCT
StyI          255  CCAAGG               C^C[AT][AT]GG
TfiI          219  GATTC                G^A[AT]TC
TseI          406  GCTGC                G^C[AT]GC
XhoII         384  GGATCT               [GA]^GATC[CT]
XmaCI         308  CCCGGG               C^CCGGG
XmaI          308  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BalI          207  TGGCCA               TGG^CCA
              518  TGGCCA              
BsaJI         255  CCAAGG               C^C[ACGT][ACGT]GG
              308  CCCGGG              
BsmAI         160  GAGAC                GTCTC(1/5)
              234  GAGAC               
BsmI          326  GAATGC               GAATGC(1/-1)
              435  GCATTC              
EaeI          207  TGGCCA               [CT]^GGCC[GA]
              518  TGGCCA              
Fnu4HI         79  GCCGC                GC^[ACGT]GC
              406  GCTGC               
HapII         309  CCGG                 C^CGG
              348  CCGG                
HpaII         309  CCGG                 C^CGG
              348  CCGG                
HphI          128  TCACC                GGTGA(8/7)
              447  TCACC               
ItaI           79  GCCGC                GC^[ACGT]GC
              406  GCTGC               
MboII          42  GAAGA                GAAGA(8/7)
              313  GAAGA               
MluNI         207  TGGCCA               TGG^CCA
              518  TGGCCA              
MscI          207  TGGCCA               TGG^CCA
              518  TGGCCA              
MspI          309  CCGG                 C^CGG
              348  CCGG                
TaqI          222  TCGA                 T^CGA
              275  TCGA                
Tsp45I        127  GTCAC                ^GT[GC]AC
              446  GTCAC               
TspRI         164  CAGTG                CA[GC]TG[ACGT][ACGT]^
              191  CAGTG               
TthHB8I       222  TCGA                 T^CGA
              275  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AfaI        AluI        BcnI        BsiYI       
BslI        BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DpnII       EcoRII      FokI        
HaeIII      MaeIII      MboI        MnlI        MvaI        
MwoI        NciI        NdeII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       
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