Restriction map for pF1KB6867
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MunI        MvnI        NaeI        NarI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        Sau3AI      ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AciI          433  CCGC                 CCGC(-3/-1)
AlwNI         202  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI         7  AGCGCT               AGC^GCT
BanI          504  GGTGCC               G^G[CT][GA]CC
BbvI          226  GCTGC                GCAGC(8/12)
BglI          507  GCCCACTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI          19  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          480  GAGACC               GGTCTC(1/5)
BsmAI         480  GAGAC                GTCTC(1/5)
BspMI         414  ACCTGC               ACCTGC(4/8)
BsrI          406  CCAGT                ACTGG(1/-1)
CfoI            8  GCGC                 GCG^C
Eco47III        7  AGCGCT               AGC^GCT
EcoNI         274  CCTCCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       437  CCATGG               C^C[AT][AT]GG
HaeII           7  AGCGCT               [GA]GCGC^[CT]
HhaI            8  GCGC                 GCG^C
HinP1I          8  GCGC                 G^CGC
MaeIII         80  GTGAC                ^GT[ACGT]AC
MamI           19  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI          437  CCATGG               C^CATGG
SapI          190  GAAGAGC              GCTCTTC(1/4)
SbfI          277  CCTGCAGG             CCTGCA^GG
Sse8387I      277  CCTGCAGG             CCTGCA^GG
StyI          437  CCATGG               C^C[AT][AT]GG
TaqI          528  TCGA                 T^CGA
TseI          226  GCTGC                G^C[AT]GC
Tsp45I         80  GTGAC                ^GT[GC]AC
TthHB8I       528  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvaII         316  GGTCC                G^G[AT]CC
              412  GGACC               
BcnI          313  CCGGG                CC^[GC]GG
              517  CCCGG               
BsmFI         411  GGGAC                GGGAC(10/14)
              494  GTCCC               
Cac8I         195  GCAGGC               GC[ACGT]^[ACGT]GC
              339  GCCAGC              
DdeI           28  CTCAG                C^T[ACGT]AG
              200  CTCAG               
DsaI           14  CCGTGG               C^C[GA][CT]GG
              437  CCATGG              
EarI           49  CTCTTC               CTCTTC(1/4)
              190  GAAGAG              
Fnu4HI        226  GCTGC                GC^[ACGT]GC
              432  GCCGC               
HapII         313  CCGG                 C^CGG
              518  CCGG                
HinfI         109  GACTC                G^A[ACGT]TC
              525  GACTC               
HpaII         313  CCGG                 C^CGG
              518  CCGG                
HphI           58  TCACC                GGTGA(8/7)
              101  TCACC               
ItaI          226  GCTGC                GC^[ACGT]GC
              432  GCCGC               
Ksp632I        49  CTCTTC               CTCTTC(1/4)
              190  GAAGAG              
MboII         190  GAAGA                GAAGA(8/7)
               50  TCTTC               
MspI          313  CCGG                 C^CGG
              518  CCGG                
MwoI          453  GCCAATGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              507  GCCCACTCGGC         
NciI          313  CCGGG                CC^[GC]GG
              517  CCCGG               
NdeI          349  CATATG               CA^TATG
              448  CATATG              
PleI          109  GACTC                GAGTC(4/5)
              525  GACTC               
PpuMI         315  GGGTCCT              [GA]G^G[AT]CC[CT]
              411  GGGACCT             
PstI          135  CTGCAG               CTGCA^G
              278  CTGCAG              
SfcI          135  CTGCAG               C^T[GA][CT]AG
              278  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

ApaI        BanII       BmyI        BsaJI       BseRI       
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       Cfr13I      DraII       EcoO109I    EcoRII      
FokI        HaeIII      MnlI        MslI        MvaI        
NlaIII      NlaIV       Sau96I      ScrFI       SfaNI       
TspRI       
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