Restriction map for pF1KB8277
 (Circular) (Six-base) MAPSORT of: pF1KB8277.seq  Check: 4386  from: 1  to: 4184



pF1KB8277  4184 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    486     486 
   Size:       4184
 
AccI GT'mk_AC

Cuts at:    166     599    1222     166 
   Size:        433     623    3128

  Fragments arranged by size: 

                3128     623     433
 
Acc65I G'GTAC_C

Cuts at:   1200    1200 
   Size:       4184
 
AclI AA'CG_TT

Cuts at:   4024    4024 
   Size:       4184
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    442    2009    2441    3188    3456     442 
   Size:       1567     432     747     268    1170

  Fragments arranged by size: 

                1567    1170     747     432     268
 
AfeI AGC'GCT

Cuts at:   1151    1151 
   Size:       4184
 
AflIII A'CryG_T

Cuts at:    878    1058    1536    2640    3617     878 
   Size:        180     478    1104     977    1445

  Fragments arranged by size: 

                1445    1104     977     478     180
 
AgeI A'CCGG_T

Cuts at:   2572    2572 
   Size:       4184
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    773     774     805     806     773 
   Size:          1      31       1    4151

  Fragments arranged by size: 

                4151      31       1       1
 
AlwNI CAG_nnn'CTG

Cuts at:    579    1489    3056     579 
   Size:        910    1567    1707

  Fragments arranged by size: 

                1707    1567     910
 
ApaLI G'TGCA_C

Cuts at:   2954    2954 
   Size:       4184
 
ApoI r'AATT_y

Cuts at:   1078    1188    3537    3793    1078 
   Size:        110    2349     256    1469

  Fragments arranged by size: 

                2349    1469     256     110
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4184
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4184
 
AvaI C'yCGr_G

Cuts at:    746    1204     746 
   Size:        458    3726
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    846     879     846 
   Size:         33    4151
 
BamHI G'GATC_C

Cuts at:   1209    1209 
   Size:       4184
 
BanI G'GyrC_C

Cuts at:   1200    1614    1862    1897    1200 
   Size:        414     248      35    3487

  Fragments arranged by size: 

                3487     414     248      35
 
BanII G_rGCy'C

Cuts at:   1198    2228    1198 
   Size:       1030    3154
 
BbeI G_GCGC'C

Cuts at:   1618    1866    1618 
   Size:        248    3936
 
BbsI GAAGACnn'nnnn_

Cuts at:    478     478 
   Size:       4184
 
BciVI GTATCCnnnnn_n'

Cuts at:   2082    2843    4184    2082 
   Size:        761    1341    2082

  Fragments arranged by size: 

                2082    1341     761
 
BclI T'GATC_A

Cuts at:    207    1025    1704     207 
   Size:        818     679    2687

  Fragments arranged by size: 

                2687     818     679
 
BglII A'GATC_T

Cuts at:   1699    3736    1699 
   Size:       2037    2147
 
BlpI GC'TnA_GC

Cuts at:    193     266    1296     193 
   Size:         73    1030    3081

  Fragments arranged by size: 

                3081    1030      73
 
Bme1580I G_kGCm'C

Cuts at:    830    1809    1902    2958     830 
   Size:        979      93    1056    2056

  Fragments arranged by size: 

                2056    1056     979      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1396    1477    1812    3956    1396 
   Size:         81     335    2144    1624

  Fragments arranged by size: 

                2144    1624     335      81
 
BmtI G_CTAG'C

Cuts at:    124     124 
   Size:       4184
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    726    2551     726 
   Size:       1825    2359
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    466     546     646     785     820     466 
   Size:         80     100     139      35    3830

  Fragments arranged by size: 

                3830     139     100      80      35
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1360    2239    2731    3029    1360 
   Size:        879     492     298    2515

  Fragments arranged by size: 

                2515     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    905    1044    3888     905 
   Size:        139    2844    1201

  Fragments arranged by size: 

                2844    1201     139
 
BsaAI yAC'GTr

Cuts at:   1537    1537 
   Size:       4184
 
BsaBI GATnn'nnATC

Cuts at:      6     805    1030    1698    3735       6 
   Size:        799     225     668    2037     455

  Fragments arranged by size: 

                2037     799     668     455     225
 
BsaHI Gr'CG_yC

Cuts at:   1615    1863    2565    4061    1615 
   Size:        248     702    1496    1738

  Fragments arranged by size: 

                1738    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1373    1894    2572    2846    2993    3404    1373 
   Size:        521     678     274     147     411    2153

  Fragments arranged by size: 

                2153     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1163    1193    1163 
   Size:         30    4154
 
BseYI C'CCAG_C

Cuts at:    139     522    1609    2367    2944    3440     139 
   Size:        383    1087     758     577     496     883

  Fragments arranged by size: 

                1087     883     758     577     496     383
 
BsiEI CG_ry'CG

Cuts at:    104    1726    1772    2980     104 
   Size:       1622      46    1208    1308

  Fragments arranged by size: 

                1622    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1198    1976    2166    2958    1198 
   Size:        778     190     792    2424

  Fragments arranged by size: 

                2424     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    947     947 
   Size:       4184
 
BsmBI CGTCTCn'nnnn_

Cuts at:    128     442     128 
   Size:        314    3870
 
Bsp1286I G_dGCh'C

Cuts at:    830    1198    1809    1902    1976    2166    2228    2958     830 
   Size:        368     611      93      74     190      62     730    2056

  Fragments arranged by size: 

                2056     730     611     368     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3404    3404 
   Size:       4184
 
BspHI T'CATG_A

Cuts at:    809    3360     809 
   Size:       2551    1633
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    486    1234    1448    1750    2131     486 
   Size:        748     214     302     381    2539

  Fragments arranged by size: 

                2539     748     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     270     819    2476    2530    4014    4179      50 
   Size:        220     549    1657      54    1484     165      55

  Fragments arranged by size: 

                1657    1484     549     220     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    360    2096     360 
   Size:       1736    2448
 
BsrFI r'CCGG_y

Cuts at:   2182    2363    2572    2182 
   Size:        181     209    3794

  Fragments arranged by size: 

                3794     209     181
 
BsrGI T'GTAC_A

Cuts at:    611     611 
   Size:       4184
 
BssSI C'ACGA_G

Cuts at:   2455    2813    2455 
   Size:        358    3826
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1561    1561 
   Size:       4184
 
BstBI TT'CG_AA

Cuts at:   2545    2545 
   Size:       4184
 
BstEII G'GTnAC_C

Cuts at:    321     321 
   Size:       4184
 
BstXI CCAn_nnnn'nTGG

Cuts at:    312     312 
   Size:       4184
 
BstYI r'GATC_y

Cuts at:      1     750    1209    1676    1699    2034    2280    3292    3378 
   Size:        749     459     467      23     335     246    1012      86

Cuts at:   3378    3390    3736       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     749     467     459     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4184
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2134    2315    2478    2134 
   Size:        181     163    3840

  Fragments arranged by size: 

                3840     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1053    1427    1053 
   Size:        374    3810
 
DraI TTT'AAA

Cuts at:   1183    1183 
   Size:       4184
 
DrdI GACnn_nn'nnGTC

Cuts at:   1890    2748    1890 
   Size:        858    3326
 
EaeI y'GGCC_r

Cuts at:    438    1769    1943    2334    2361    3706     438 
   Size:       1331     174     391      27    1345     916

  Fragments arranged by size: 

                1345    1331     916     391     174      27
 
EagI C'GGCC_G

Cuts at:   1769    1769 
   Size:       4184
 
EarI CTCTTCn'nnn_

Cuts at:    690     766    2207    2417     690 
   Size:         76    1441     210    2457

  Fragments arranged by size: 

                2457    1441     210      76
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2392    2702    2848    3994    2392 
   Size:        310     146    1146    2582

  Fragments arranged by size: 

                2582    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1196    1196 
   Size:       4184
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    442     466     546     646     785     820    2009    2441    3188 
   Size:         24      80     100     139      35    1189     432     747

Cuts at:   3188    3456     442 
   Size:        268    1170

  Fragments arranged by size: 

                1189    1170     747     432     268     139     100      80
                  35      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:    685     685 
   Size:       4184
 
EcoO109I rG'GnC_Cy

Cuts at:   1020    1323    1020 
   Size:        303    3881
 
EcoRI G'AATT_C

Cuts at:   1078    1188    1078 
   Size:        110    4074
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    171     213     447    2203     171 
   Size:         42     234    1756    2152

  Fragments arranged by size: 

                2152    1756     234      42
 
FspI TGC'GCA

Cuts at:   1965    1965 
   Size:       4184
 
HaeII r_GCGC'y

Cuts at:   1153    1618    1866    2888    3867    1153 
   Size:        465     248    1022     979    1470

  Fragments arranged by size: 

                1470    1022     979     465     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    465     497     523     555    1004    1036    2026    2056    2058 
   Size:         32      26      32     449      32     990      30       2

Cuts at:   2058    2088     465 
   Size:         30    2561

  Fragments arranged by size: 

                2561     990     449      32      32      32      30      30
                  26       2
 
HincII GTy'rAC

Cuts at:    333    1223     333 
   Size:        890    3294
 
HindIII A'AGCT_T

Cuts at:    506     506 
   Size:       4184
 
KasI G'GCGC_C

Cuts at:   1614    1862    1614 
   Size:        248    3936
 
KpnI G_GTAC'C

Cuts at:   1204    1204 
   Size:       4184
 
MluI A'CGCG_T

Cuts at:   3617    3617 
   Size:       4184
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    863    2855    3039     863 
   Size:       1992     184    2008

  Fragments arranged by size: 

                2008    1992     184
 
MscI TGG'CCA

Cuts at:    440    1945     440 
   Size:       1505    2679
 
MslI CAynn'nnrTG

Cuts at:   2173    2300    2173 
   Size:        127    4057
 
MspA1I CmG'CkG

Cuts at:   1295    1609    1969    2982    3227    1295 
   Size:        314     360    1013     245    2252

  Fragments arranged by size: 

                2252    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2365    2365 
   Size:       4184
 
NarI GG'CG_CC

Cuts at:   1615    1863    1615 
   Size:        248    3936
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4184
 
NdeI CA'TA_TG

Cuts at:    231     231 
   Size:       4184
 
NgoMIV G'CCGG_C

Cuts at:   2363    2363 
   Size:       4184
 
NheI G'CTAG_C

Cuts at:    120     120 
   Size:       4184
 
NspI r_CATG'y

Cuts at:    882    1062    1237    1737    2644     882 
   Size:        180     175     500     907    2422

  Fragments arranged by size: 

                2422     907     500     180     175
 
PciI A'CATG_T

Cuts at:    878    1058    2640     878 
   Size:        180    1582    2422

  Fragments arranged by size: 

                2422    1582     180
 
PmeI GTTT'AAAC

Cuts at:   1183    1183 
   Size:       4184
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3323    3355    3323 
   Size:         32    4152
 
PpuMI rG'GwC_Cy

Cuts at:   1020    1020 
   Size:       4184
 
PstI C_TGCA'G

Cuts at:    584    1231    1916     584 
   Size:        647     685    2852

  Fragments arranged by size: 

                2852     685     647
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4184
 
PvuII CAG'CTG

Cuts at:   1609    1969    1609 
   Size:        360    3824
 
RsrII CG'GwC_CG

Cuts at:   2379    2379 
   Size:       4184
 
SacI G_AGCT'C

Cuts at:   1198    1198 
   Size:       4184
 
SalI G'TCGA_C

Cuts at:   1221    1221 
   Size:       4184
 
SapI GCTCTTCn'nnn_

Cuts at:    690    2207    2417     690 
   Size:       1517     210    2457

  Fragments arranged by size: 

                2457    1517     210
 
SbfI CC_TGCA'GG

Cuts at:   1231    1231 
   Size:       4184
 
ScaI AGT'ACT

Cuts at:   3701    3701 
   Size:       4184
 
SexAI A'CCwGG_T

Cuts at:    935     935 
   Size:       4184
 
SfcI C'TryA_G

Cuts at:     33     580    1227    1912    2905    3096      33 
   Size:        547     647     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     647     547     191
 
SfoI GGC'GCC

Cuts at:   1616    1864    1616 
   Size:        248    3936
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4184
 
SmaI CCC'GGG

Cuts at:   1206    1206 
   Size:       4184
 
SmlI C'TyrA_G

Cuts at:   1339    2254    2746    3008    1339 
   Size:        915     492     262    2515

  Fragments arranged by size: 

                2515     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4184
 
SphI G_CATG'C

Cuts at:   1237    1737    1237 
   Size:        500    3684
 
StyI C'CwwG_G

Cuts at:    107    1318    1672     107 
   Size:       1211     354    2619

  Fragments arranged by size: 

                2619    1211     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    829    1767    1900    2359    2568     829 
   Size:        938     133     459     209    2445

  Fragments arranged by size: 

                2445     938     459     209     133
 
TatI w'GTAC_w

Cuts at:    611    1106    3699     611 
   Size:        495    2593    1096

  Fragments arranged by size: 

                2593    1096     495
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3701    3733    3701 
   Size:         32    4152
 
Tth111I GACn'n_nGTC

Cuts at:   1981    1981 
   Size:       4184
 
XbaI T'CTAG_A

Cuts at:   1215    1215 
   Size:       4184
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    519    1679     519 
   Size:       1160    3024
 
XmaI C'CCGG_G

Cuts at:   1204    1204 
   Size:       4184
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NspI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII    AflII     AhdI     AleI     AlfI     ApaI     AscI    AvrII
    BbvCI     BcgI    BfrBI     BglI    BmgBI   Bpu10I    BseRI     BsgI
    BsiWI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoRV
     FseI    FspAI     HpaI     MfeI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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