Restriction map for pF1KB6828
##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanII       
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

BalI          340  TGGCCA               TGG^CCA
BanI          483  GGTGCC               G^G[CT][GA]CC
BbvCI         419  CCTCAGC              CCTCAGC(-5/-2)
BbvI          433  GCTGC                GCAGC(8/12)
BfaI           95  CTAG                 C^TAG
BmyI          484  GTGCCC               G[AGT]GC[ACT]^C
BpmI           37  CTGGAG               CTGGAG(16/14)
BseRI          21  CTCCTC               GAGGAG(10/8)
BsmBI          40  GAGACG               CGTCTC(1/5)
BsmFI         226  GGGAC                GGGAC(10/14)
Bsp1286I      484  GTGCCC               G[AGT]GC[ACT]^C
BsrBI         196  CCGCTC               CCGCTC(-3/-3)
BsrDI         408  CATTGC               GCAATG(2/0)
BssKI          36  CCTGG                ^CC[ACGT]GG
BssSI          24  CTCGTG               CACGAG(-5/-1)
BstNI          36  CCTGG                CC^[AT]GG
BstXI         366  CCATAGACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         397  GCAAGC               GC[ACGT]^[ACGT]GC
DpnII          15  GATC                 ^GATC
DraI          471  TTTAAA               TTT^AAA
DsaI          290  CCATGG               C^C[GA][CT]GG
EaeI          340  TGGCCA               [CT]^GGCC[GA]
EcoRII         36  CCTGG                ^CC[AT]GG
Fnu4HI        433  GCTGC                GC^[ACGT]GC
HaeIII        341  GGCC                 GG^CC
HinfI         514  GAGTC                G^A[ACGT]TC
HphI          186  GGTGA                GGTGA(8/7)
ItaI          433  GCTGC                GC^[ACGT]GC
MaeI           95  CTAG                 C^TAG
MaeIII        323  GTGAC                ^GT[ACGT]AC
MboI           15  GATC                 ^GATC
MluNI         340  TGGCCA               TGG^CCA
MscI          340  TGGCCA               TGG^CCA
MseI          472  TTAA                 T^TAA
MvaI           36  CCTGG                CC^[AT]GG
NcoI          290  CCATGG               C^CATGG
NdeI          489  CATATG               CA^TATG
NdeII          15  GATC                 ^GATC
NlaIII        291  CATG                 CATG^
PleI          514  GAGTC                GAGTC(4/5)
Sau3AI         15  GATC                 ^GATC
ScrFI          36  CCTGG                CC^[ACGT]GG
TaqI            4  TCGA                 T^CGA
Tru9I         472  TTAA                 T^TAA
TseI          433  GCTGC                G^C[AT]GC
Tsp45I        323  GTGAC                ^GT[GC]AC
Tsp509I        47  AATT                 ^AATT
TthHB8I         4  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI          196  CCGC                 CCGC(-3/-1)
              295  GCGG                
BsaJI         290  CCATGG               C^C[ACGT][ACGT]GG
              479  CCAAGG              
BsiYI          63  CCCATTAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              196  CCGCTCTGTGG         
BslI           63  CCCATTAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              196  CCGCTCTGTGG         
BsmAI          40  GAGAC                GTCTC(1/5)
              175  GTCTC               
BsrI          343  CCAGT                ACTGG(1/-1)
              385  CCAGT               
BstF5I        554  CATCC                GGATG(2/0)
              108  GGATG               
DdeI          267  CTTAG                C^T[ACGT]AG
              420  CTCAG               
EarI          118  GAAGAG               CTCTTC(1/4)
              511  GAAGAG              
EcoT14I       290  CCATGG               C^C[AT][AT]GG
              479  CCAAGG              
FokI          554  CATCC                GGATG(9/13)
              108  GGATG               
Ksp632I       118  GAAGAG               CTCTTC(1/4)
              511  GAAGAG              
MboII         118  GAAGA                GAAGA(8/7)
              511  GAAGA               
MslI          354  CATAGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              408  CATTGCTGTG          
NlaIV         381  GGTTCC               GG[ACGT]^[ACGT]CC
              483  GGTGCC              
SfaNI         436  GCATC                GCATC(5/9)
              553  GCATC               
StyI          290  CCATGG               C^C[AT][AT]GG
              479  CCAAGG              
TspRI         359  CAGTG                CA[GC]TG[ACGT][ACGT]^
              386  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AluI        MnlI        MwoI        
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