Restriction map for pF1KB6849
 (Circular) (Six-base) MAPSORT of: pF1KB6849.seq  Check: 5984  from: 1  to: 3644



pF1KB6849  3644 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:    682     682 
   Size:       3644
 
Acc65I G'GTAC_C

Cuts at:    660     660 
   Size:       3644
 
AclI AA'CG_TT

Cuts at:   3484    3484 
   Size:       3644
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1469    1901    2648    2916    1469 
   Size:        432     747     268    2197

  Fragments arranged by size: 

                2197     747     432     268
 
AflIII A'CryG_T

Cuts at:    996    2100    3077     996 
   Size:       1104     977    1563

  Fragments arranged by size: 

                1563    1104     977
 
AgeI A'CCGG_T

Cuts at:   2032    2032 
   Size:       3644
 
AleI CACnn'nnGTG

Cuts at:    190     373     190 
   Size:        183    3461
 
AlwNI CAG_nnn'CTG

Cuts at:    150     162     949    2516     150 
   Size:         12     787    1567    1278

  Fragments arranged by size: 

                1567    1278     787      12
 
ApaLI G'TGCA_C

Cuts at:    183    2414     183 
   Size:       2231    1413
 
ApoI r'AATT_y

Cuts at:    534     648    2997    3253     534 
   Size:        114    2349     256     925

  Fragments arranged by size: 

                2349     925     256     114
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3644
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3644
 
AvaI C'yCGr_G

Cuts at:    664     664 
   Size:       3644
 
BamHI G'GATC_C

Cuts at:    669     669 
   Size:       3644
 
BanI G'GyrC_C

Cuts at:    660    1074    1322    1357     660 
   Size:        414     248      35    2947

  Fragments arranged by size: 

                2947     414     248      35
 
BanII G_rGCy'C

Cuts at:    658    1688     658 
   Size:       1030    2614
 
BbeI G_GCGC'C

Cuts at:   1078    1326    1078 
   Size:        248    3396
 
BbsI GAAGACnn'nnnn_

Cuts at:    118     118 
   Size:       3644
 
BciVI GTATCCnnnnn_n'

Cuts at:   1542    2303    3644    1542 
   Size:        761    1341    1542

  Fragments arranged by size: 

                1542    1341     761
 
BclI T'GATC_A

Cuts at:   1164    1164 
   Size:       3644
 
BglII A'GATC_T

Cuts at:   1159    3196    1159 
   Size:       2037    1607
 
BlpI GC'TnA_GC

Cuts at:    756     756 
   Size:       3644
 
Bme1580I G_kGCm'C

Cuts at:    187    1269    1362    2418     187 
   Size:       1082      93    1056    1413

  Fragments arranged by size: 

                1413    1082    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    856     937    1272    3416     856 
   Size:         81     335    2144    1084

  Fragments arranged by size: 

                2144    1084     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2011    2011 
   Size:       3644
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    312     312 
   Size:       3644
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    820    1699    2191    2489     820 
   Size:        879     492     298    1975

  Fragments arranged by size: 

                1975     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    200     594    3348     200 
   Size:        394    2754     496

  Fragments arranged by size: 

                2754     496     394
 
BsaAI yAC'GTr

Cuts at:    997     997 
   Size:       3644
 
BsaBI GATnn'nnATC

Cuts at:      6    1158    3195       6 
   Size:       1152    2037     455

  Fragments arranged by size: 

                2037    1152     455
 
BsaHI Gr'CG_yC

Cuts at:   1075    1323    2025    3521    1075 
   Size:        248     702    1496    1198

  Fragments arranged by size: 

                1496    1198     702     248
 
BsaWI w'CCGG_w

Cuts at:    833    1354    2032    2306    2453    2864     833 
   Size:        521     678     274     147     411    1613

  Fragments arranged by size: 

                1613     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    247     407    1069    1827    2404    2900     247 
   Size:        160     662     758     577     496     991

  Fragments arranged by size: 

                 991     758     662     577     496     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    397     526     397 
   Size:        129    3515
 
BsiEI CG_ry'CG

Cuts at:    104    1186    1232    2440     104 
   Size:       1082      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1082      46
 
BsiHKAI G_wGCw'C

Cuts at:    187     658    1436    1626    2418     187 
   Size:        471     778     190     792    1413

  Fragments arranged by size: 

                1413     792     778     471     190
 
Bsp1286I G_dGCh'C

Cuts at:    187     658    1269    1362    1436    1626    1688    2418     187 
   Size:        471     611      93      74     190      62     730    1413

  Fragments arranged by size: 

                1413     730     611     471     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2864    2864 
   Size:       3644
 
BspHI T'CATG_A

Cuts at:    308    2820     308 
   Size:       2512    1132
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    218     694     908    1210    1591     218 
   Size:        476     214     302     381    2271

  Fragments arranged by size: 

                2271     476     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1936    1990    3474    3639      50 
   Size:       1886      54    1484     165      55

  Fragments arranged by size: 

                1886    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1556    1556 
   Size:       3644
 
BsrFI r'CCGG_y

Cuts at:    265    1642    1823    2032     265 
   Size:       1377     181     209    1877

  Fragments arranged by size: 

                1877    1377     209     181
 
BssSI C'ACGA_G

Cuts at:   1915    2273    1915 
   Size:        358    3286
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1021    1021 
   Size:       3644
 
BstBI TT'CG_AA

Cuts at:   2005    2005 
   Size:       3644
 
BstXI CCAn_nnnn'nTGG

Cuts at:    255     255 
   Size:       3644
 
BstYI r'GATC_y

Cuts at:      1     323     669    1136    1159    1494    1740    2752    2838 
   Size:        322     346     467      23     335     246    1012      86

Cuts at:   2838    2850    3196       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     346     335     322     246
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    347    1594    1775    1938     347 
   Size:       1247     181     163    2053

  Fragments arranged by size: 

                2053    1247     181     163
 
BtsI GCAGTG_nn'

Cuts at:    887     887 
   Size:       3644
 
DraI TTT'AAA

Cuts at:    643     643 
   Size:       3644
 
DrdI GACnn_nn'nnGTC

Cuts at:   1350    2208    1350 
   Size:        858    2786
 
EaeI y'GGCC_r

Cuts at:    635    1229    1403    1794    1821    3166     635 
   Size:        594     174     391      27    1345    1113

  Fragments arranged by size: 

                1345    1113     594     391     174      27
 
EagI C'GGCC_G

Cuts at:   1229    1229 
   Size:       3644
 
EarI CTCTTCn'nnn_

Cuts at:   1667    1877    1667 
   Size:        210    3434
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1852    2162    2308    3454    1852 
   Size:        310     146    1146    2042

  Fragments arranged by size: 

                2042    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    656     656 
   Size:       3644
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    312    1469    1901    2648    2916     312 
   Size:       1157     432     747     268    1040

  Fragments arranged by size: 

                1157    1040     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    783     783 
   Size:       3644
 
EcoRI G'AATT_C

Cuts at:    648     648 
   Size:       3644
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1663    1663 
   Size:       3644
 
FspI TGC'GCA

Cuts at:   1425    1425 
   Size:       3644
 
HaeII r_GCGC'y

Cuts at:    151    1078    1326    2348    3327     151 
   Size:        927     248    1022     979     468

  Fragments arranged by size: 

                1022     979     927     468     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1486    1516    1518    1548    1486 
   Size:         30       2      30    3582

  Fragments arranged by size: 

                3582      30      30       2
 
HincII GTy'rAC

Cuts at:    683     683 
   Size:       3644
 
KasI G'GCGC_C

Cuts at:   1074    1322    1074 
   Size:        248    3396
 
KpnI G_GTAC'C

Cuts at:    664     664 
   Size:       3644
 
MluI A'CGCG_T

Cuts at:   3077    3077 
   Size:       3644
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2315    2499    2315 
   Size:        184    3460
 
MscI TGG'CCA

Cuts at:   1405    1405 
   Size:       3644
 
MslI CAynn'nnrTG

Cuts at:    190     373    1633    1760     190 
   Size:        183    1260     127    2074

  Fragments arranged by size: 

                2074    1260     183     127
 
MspA1I CmG'CkG

Cuts at:    755    1069    1429    2442    2687     755 
   Size:        314     360    1013     245    1712

  Fragments arranged by size: 

                1712    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1825    1825 
   Size:       3644
 
NarI GG'CG_CC

Cuts at:   1075    1323    1075 
   Size:        248    3396
 
NgoMIV G'CCGG_C

Cuts at:   1823    1823 
   Size:       3644
 
NspI r_CATG'y

Cuts at:    697    1197    2104     697 
   Size:        500     907    2237

  Fragments arranged by size: 

                2237     907     500
 
PciI A'CATG_T

Cuts at:   2100    2100 
   Size:       3644
 
PmeI GTTT'AAAC

Cuts at:    643     643 
   Size:       3644
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2783    2815    2783 
   Size:         32    3612
 
PstI C_TGCA'G

Cuts at:    215     691    1376     215 
   Size:        476     685    2483

  Fragments arranged by size: 

                2483     685     476
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3644
 
PvuII CAG'CTG

Cuts at:   1069    1429    1069 
   Size:        360    3284
 
RsrII CG'GwC_CG

Cuts at:   1839    1839 
   Size:       3644
 
SacI G_AGCT'C

Cuts at:    658     658 
   Size:       3644
 
SalI G'TCGA_C

Cuts at:    681     681 
   Size:       3644
 
SapI GCTCTTCn'nnn_

Cuts at:   1667    1877    1667 
   Size:        210    3434
 
SbfI CC_TGCA'GG

Cuts at:    691     691 
   Size:       3644
 
ScaI AGT'ACT

Cuts at:   3161    3161 
   Size:       3644
 
SfcI C'TryA_G

Cuts at:     33     211     687    1372    2365    2556      33 
   Size:        178     476     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     476     191     178
 
SfoI GGC'GCC

Cuts at:   1076    1324    1076 
   Size:        248    3396
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3644
 
SmaI CCC'GGG

Cuts at:    666     666 
   Size:       3644
 
SmlI C'TyrA_G

Cuts at:    799    1714    2206    2468     799 
   Size:        915     492     262    1975

  Fragments arranged by size: 

                1975     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3644
 
SphI G_CATG'C

Cuts at:    697    1197     697 
   Size:        500    3144
 
StyI C'CwwG_G

Cuts at:    318     778    1132     318 
   Size:        460     354    2830

  Fragments arranged by size: 

                2830     460     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    180     559    1227    1360    1819    2028     180 
   Size:        379     668     133     459     209    1796

  Fragments arranged by size: 

                1796     668     459     379     209     133
 
TatI w'GTAC_w

Cuts at:   3159    3159 
   Size:       3644
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3161    3193    3161 
   Size:         32    3612
 
Tth111I GACn'n_nGTC

Cuts at:   1441    1441 
   Size:       3644
 
XbaI T'CTAG_A

Cuts at:    675     675 
   Size:       3644
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    507    1139     507 
   Size:        632    3012
 
XmaI C'CCGG_G

Cuts at:    664     664 
   Size:       3644
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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