Restriction map for pF1KB6733
 (Linear) (Six-base) MAPSORT of: KIBB6733.seq  Check: 6371  from: 1  to: 285



KIBB6733.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     225     285 
   Size:        225      60
 
AccI GT'mk_AC

Cuts at:      0     281     285 
   Size:        281       4
 
AseI AT'TA_AT

Cuts at:      0      53     285 
   Size:         53     232
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     203     285 
   Size:        109      94      82

  Fragments arranged by size: 

                 109      94      82
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     225     285 
   Size:        225      60
 
EaeI y'GGCC_r

Cuts at:      0       2     285 
   Size:          2     283
 
MfeI C'AATT_G

Cuts at:      0     248     285 
   Size:        248      37
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     285 
   Size:        206      79
 
StyI C'CwwG_G

Cuts at:      0      17     285 
   Size:         17     268
 

 Enzymes that do cut:

     AarI     AccI     AseI     BsgI    BspMI     EaeI     MfeI     MmeI
     StyI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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