Restriction map for pF1KB6484
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BmyI        
BpmI        BpuAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraIII      EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MflI        
MluI        MroI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcsI         1012  AAATTC               [GA]^AATT[CT]
AfaI          120  GTAC                 GT^AC
AflIII        581  ACGTGT               A^C[GA][CT]GT
AlwI          513  GATCC                GGATC(4/5)
AlwNI         131  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI         1012  AAATTC               [GA]^AATT[CT]
AvaII         124  GGACC                G^G[AT]CC
BalI         1007  TGGCCA               TGG^CCA
BbrPI         580  CACGTG               CAC^GTG
BfaI          290  CTAG                 C^TAG
BlpI          430  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      430  GCTCAGC              GC^T[ACGT]AGC
BsgI           88  GTGCAG               GTGCAG(16/14)
BsmFI          39  GGGAC                GGGAC(10/14)
BspMI         472  ACCTGC               ACCTGC(4/8)
BsrDI         258  CATTGC               GCAATG(2/0)
BsrI          630  ACTGG                ACTGG(1/-1)
BssKI          84  CCTGG                ^CC[ACGT]GG
BstAPI        435  GCATGCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1044  GGATG                GGATG(2/0)
BstNI          84  CCTGG                CC^[AT]GG
BstXI        1010  CCAAATTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         430  GCTCAGC              GC^T[ACGT]AGC
DraI          385  TTTAAA               TTT^AAA
DraII         878  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI          310  GACCGAGTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          452  CCATGG               C^C[GA][CT]GG
EaeI         1007  TGGCCA               [CT]^GGCC[GA]
EcoO109I      878  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRII         84  CCTGG                ^CC[AT]GG
FokI         1044  GGATG                GGATG(9/13)
HincII        409  GTTGAC               GT[CT]^[GA]AC
HindII        409  GTTGAC               GT[CT]^[GA]AC
HinfI         615  GAATC                G^A[ACGT]TC
MaeI          290  CTAG                 C^TAG
MluNI        1007  TGGCCA               TGG^CCA
MscI         1007  TGGCCA               TGG^CCA
MslI          901  CATAATTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           84  CCTGG                CC^[AT]GG
NcoI          452  CCATGG               C^CATGG
NspI          435  GCATGC               [GA]CATG^[CT]
PmaCI         580  CACGTG               CAC^GTG
PmlI          580  CACGTG               CAC^GTG
RsaI          120  GTAC                 GT^AC
ScrFI          84  CCTGG                CC^[ACGT]GG
SexAI          83  ACCTGGT              A^CC[AT]GGT
SphI          435  GCATGC               GCATG^C
TfiI          615  GAATC                G^A[AT]TC
XcmI          983  CCATTTATCACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI          468  GTCTAC               GT^[AC][GT]AC
              962  GTCTAC              
BsaAI         167  TACGTG               [CT]AC^GT[GA]
              580  CACGTG              
BsaJI          35  CCTTGG               C^C[ACGT][ACGT]GG
              452  CCATGG              
BsmAI         742  GAGAC                GTCTC(1/5)
              769  GAGAC               
EcoT14I        35  CCTTGG               C^C[AT][AT]GG
              452  CCATGG              
HphI           97  GGTGA                GGTGA(8/7)
              419  TCACC               
MaeII         168  ACGT                 A^CGT
              581  ACGT                
MseI          284  TTAA                 T^TAA
              386  TTAA                
MspA1I         73  CAGCTG               C[AC]G^C[GT]G
              967  CAGCTG              
NlaIV          80  GGAACC               GG[ACGT]^[ACGT]CC
              809  GGCTCC              
PvuII          73  CAGCTG               CAG^CTG
              967  CAGCTG              
StyI           35  CCTTGG               C^C[AT][AT]GG
              452  CCATGG              
Tru9I         284  TTAA                 T^TAA
              386  TTAA                
Tsp45I         98  GTGAC                ^GT[GC]AC
              308  GTGAC               
TspRI          91  CAGTG                CA[GC]TG[ACGT][ACGT]^
              341  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AciI        AluI        BbvI        BsiYI       BslI        
Cac8I       Cfr13I      DdeI        DpnII       Fnu4HI      
HaeIII      ItaI        MaeIII      MboI        MboII       
MnlI        MwoI        NdeII       NlaIII      PstI        
Sau3AI      Sau96I      SfaNI       SfcI        TseI        
Tsp509I     
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