Restriction map for pF1KB6474
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbvCI       BbvI        BcgI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssHII      Bst1107I    BstAPI      
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
Fnu4HI      FseI        FspI        HaeII       HaeIII      
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       ItaI        KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspI        
StuI        StyI        SwaI        TseI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI          616  GTCTAC               GT^[AC][GT]AC
Asp700I       867  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         819  GGATCC               G^GATCC
BanII        1060  GAGCTC               G[GA]GC[CT]^C
BciVI         210  GTATCC               GTATCC(6/5)
BclI          546  TGATCA               T^GATCA
BsaI          833  GAGACC               GGTCTC(1/5)
BsaJI         578  CCTGGG               C^C[ACGT][ACGT]GG
Bsp1407I     1007  TGTACA               T^GTACA
BsrGI        1007  TGTACA               T^GTACA
BssSI         799  CTCGTG               CACGAG(-5/-1)
BstBI         914  TTCGAA               TT^CGAA
BstXI         852  CCATCCCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           16  GCGC                 GCG^C
DraI          430  TTTAAA               TTT^AAA
DraII         519  GGGACCT              [GA]G^G[ACGT]CC[CT]
EarI           46  CTCTTC               CTCTTC(1/4)
EcoNI         823  CCTGAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      519  GGGACCT              [GA]G^G[ACGT]CC[CT]
FbaI          546  TGATCA               T^GATCA
HhaI           16  GCGC                 GCG^C
HinP1I         16  GCGC                 G^CGC
Ksp632I        46  CTCTTC               CTCTTC(1/4)
MaeIII        949  GTGAC                ^GT[ACGT]AC
MwoI            9  GCTGATTGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          750  GCATGC               [GA]CATG^[CT]
NspV          914  TTCGAA               TT^CGAA
PleI          883  GACTC                GAGTC(4/5)
PpuMI         519  GGGACCT              [GA]G^G[AT]CC[CT]
SacI         1060  GAGCTC               GAGCT^C
SfcI          621  CTATAG               C^T[GA][CT]AG
SfuI          914  TTCGAA               TT^CGAA
SphI          750  GCATGC               GCATG^C
SspBI        1007  TGTACA               T^GTACA
Tsp45I        949  GTGAC                ^GT[GC]AC
XmnI          867  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          316  AAATTT               [GA]^AATT[CT]
              433  AAATTT              
ApoI          316  AAATTT               [GA]^AATT[CT]
              433  AAATTT              
AspHI         526  GAGCAC               G[AT]GC[AT]^C
             1060  GAGCTC              
AvaII         264  GGACC                G^G[AT]CC
              520  GGACC               
BbsI          157  GAAGAC               GAAGAC(2/6)
               63  GTCTTC              
BmyI          526  GAGCAC               G[AGT]GC[ACT]^C
             1060  GAGCTC              
BpuAI         157  GAAGAC               GAAGAC(2/6)
               63  GTCTTC              
BsiHKAI       526  GAGCAC               G[AT]GC[AT]^C
             1060  GAGCTC              
BsmAI         826  GAGAC                GTCTC(1/5)
              833  GAGAC               
Bsp1286I      526  GAGCAC               G[AGT]GC[ACT]^C
             1060  GAGCTC              
BstYI         279  AGATCC               [GA]^GATC[CT]
              819  GGATCC              
Cac8I         720  GCTGGC               GC[ACGT]^[ACGT]GC
              750  GCATGC              
Cfr13I        264  GGACC                G^G[ACGT]CC
              520  GGACC               
MflI          279  AGATCC               [GA]^GATC[CT]
              819  GGATCC              
Sau96I        264  GGACC                G^G[ACGT]CC
              520  GGACC               
XcmI          377  CCATGTACCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              410  CCAAGTCCACAGTGG     
XhoII         279  AGATCC               [GA]^GATC[CT]
              819  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AfaI        AluI        AlwI        BsiYI       BslI        
BsmFI       BsrI        BssKI       BstF5I      BstNI       
DdeI        DpnII       EcoRII      FokI        HinfI       
HphI        MboI        MboII       MnlI        MseI        
MslI        MvaI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      ScrFI       SfaNI       TaqI        
TfiI        Tru9I       Tsp509I     TspRI       TthHB8I     
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