Restriction map for pF1KB6430
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AgeI        Alw44I      
AlwI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BalI        BamHI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        HphI        KpnI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SalI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AhdI          391  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         322  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          687  CCTGAGG              CC^T[ACGT]AGG
AspEI         391  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI           54  CTCGGG               C^[CT]CG[GA]G
BglI          535  GCCAATGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         509  AGATCT               A^GATCT
BsgI          868  GTGCAG               GTGCAG(16/14)
BsmFI         123  GTCCC                GGGAC(10/14)
BsoBI          54  CTCGGG               C^[CT]CG[GA]G
BssHII        627  GCGCGC               G^CGCGC
BssSI         775  CTCGTG               CACGAG(-5/-1)
BstYI         509  AGATCT               [GA]^GATC[CT]
Bsu36I        687  CCTGAGG              CC^T[ACGT]AGG
EagI          132  CGGCCG               C^GGCCG
Eam1105I      391  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         132  CGGCCG               C^GGCCG
Eco52I        132  CGGCCG               C^GGCCG
Eco81I        687  CCTGAGG              CC^T[ACGT]AGG
EcoNI         462  CCTGAACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI         202  GACTC                G^A[ACGT]TC
MflI          509  AGATCT               [GA]^GATC[CT]
NcoI          158  CCATGG               C^CATGG
PflMI         762  CCAGGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          202  GACTC                GAGTC(4/5)
PpuMI         470  AGGACCT              [GA]G^G[AT]CC[CT]
SapI         1017  GAAGAGC              GCTCTTC(1/4)
SbfI          790  CCTGCAGG             CCTGCA^GG
SfaNI         618  GATGC                GCATC(5/9)
Sse8387I      790  CCTGCAGG             CCTGCA^GG
StuI          787  AGGCCT               AGG^CCT
TaqI          194  TCGA                 T^CGA
TthHB8I       194  TCGA                 T^CGA
Van91I        762  CCAGGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         509  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII        648  ACACGT               A^C[GA][CT]GT
              857  ACACGT              
ApaI           59  GGGCCC               GGGCC^C
              698  GGGCCC              
AspHI         772  GAGCTC               G[AT]GC[AT]^C
             1020  GAGCTC              
BbeI           27  GGCGCC               GGCGC^C
              231  GGCGCC              
BbsI            3  GAAGAC               GAAGAC(2/6)
              915  GTCTTC              
BcnI          125  CCCGG                CC^[GC]GG
              130  CCCGG               
BpuAI           3  GAAGAC               GAAGAC(2/6)
              915  GTCTTC              
BsaI           76  GAGACC               GGTCTC(1/5)
              781  GAGACC              
BsiEI         132  CGGCCG               CG[GA][CT]^CG
              992  CGGTCG              
BsiHKAI       772  GAGCTC               G[AT]GC[AT]^C
             1020  GAGCTC              
BspMI         317  ACCTGC               ACCTGC(4/8)
              473  ACCTGC              
BstAPI        180  GCAGTTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              321  GCAGACCCTGC         
DpnII         510  GATC                 ^GATC
              723  GATC                
EaeI          132  CGGCCG               [CT]^GGCC[GA]
              533  CGGCCA              
HgaI            6  GACGC                GACGC(5/10)
              407  GACGC               
KasI           27  GGCGCC               G^GCGCC
              231  GGCGCC              
MaeIII        652  GTGAC                ^GT[ACGT]AC
              979  GTCAC               
MboI          510  GATC                 ^GATC
              723  GATC                
MslI          153  CATCGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              573  CACGTGCGTG          
NarI           27  GGCGCC               GG^CGCC
              231  GGCGCC              
NciI          125  CCCGG                CC^[GC]GG
              130  CCCGG               
NdeII         510  GATC                 ^GATC
              723  GATC                
PvuII         821  CAGCTG               CAG^CTG
              971  CAGCTG              
SacI          772  GAGCTC               GAGCT^C
             1020  GAGCTC              
Sau3AI        510  GATC                 ^GATC
              723  GATC                
SfoI           27  GGCGCC               GGC^GCC
              231  GGCGCC              
Tsp45I        652  GTGAC                ^GT[GC]AC
              979  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaII       
BanI        BanII       BbrPI       BbvI        BmyI        
BpmI        BsaAI       BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HpaII       ItaI        
Ksp632I     KspI        MaeII       MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NlaIII      NlaIV       PmaCI       PmlI        PstI        
SacII       Sau96I      ScrFI       SfcI        StyI        
TseI        TspRI       XcmI        
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