Restriction map for pF1KB6430
 (Linear) (Six-base) MAPSORT of: KIBB6430.seq  Check: 3330  from: 1  to: 1029



KIBB6430.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AflIII A'CryG_T

Cuts at:      0     648     857    1029 
   Size:        648     209     172

  Fragments arranged by size: 

                 648     209     172
 
AhdI GACnn_n'nnGTC

Cuts at:      0     396    1029 
   Size:        396     633
 
AleI CACnn'nnGTG

Cuts at:      0     577    1029 
   Size:        577     452
 
AlwNI CAG_nnn'CTG

Cuts at:      0     327    1029 
   Size:        327     702
 
ApaI G_GGCC'C

Cuts at:      0      63     702    1029 
   Size:         63     639     327

  Fragments arranged by size: 

                 639     327      63
 
AvaI C'yCGr_G

Cuts at:      0      54    1029 
   Size:         54     975
 
BanI G'GyrC_C

Cuts at:      0      27     231     831    1029 
   Size:         27     204     600     198

  Fragments arranged by size: 

                 600     204     198      27
 
BanII G_rGCy'C

Cuts at:      0      63      91     146     702     776     893    1024    1029 
   Size:         63      28      55     556      74     117     131       5

  Fragments arranged by size: 

                 556     131     117      74      63      55      28       5
            
 
BbeI G_GCGC'C

Cuts at:      0      31     235    1029 
   Size:         31     204     794

  Fragments arranged by size: 

                 794     204      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     908    1029 
   Size:         10     898     121

  Fragments arranged by size: 

                 898     121      10
 
BglI GCCn_nnn'nGGC

Cuts at:      0     541    1029 
   Size:        541     488
 
BglII A'GATC_T

Cuts at:      0     509    1029 
   Size:        509     520
 
Bme1580I G_kGCm'C

Cuts at:      0      63     574     702    1029 
   Size:         63     511     128     327

  Fragments arranged by size: 

                 511     327     128      63
 
BmgBI CAC'GTC

Cuts at:      0     768    1029 
   Size:        768     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     586     622     860    1004    1029 
   Size:        355     231      36     238     144      25

  Fragments arranged by size: 

                 355     238     231     144      36      25
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      70     775    1029 
   Size:         70     705     254

  Fragments arranged by size: 

                 705     254      70
 
BsaAI yAC'GTr

Cuts at:      0     168     575     651     860    1029 
   Size:        168     407      76     209     169

  Fragments arranged by size: 

                 407     209     169     168      76
 
BsaHI Gr'CG_yC

Cuts at:      0       7      28     232     408    1029 
   Size:          7      21     204     176     621

  Fragments arranged by size: 

                 621     204     176      21       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     398     428     463     493     559     589     977    1007 
   Size:        398      30      35      30      66      30     388      30

Cuts at:   1007    1029 
   Size:         22

  Fragments arranged by size: 

                 398     388      66      35      30      30      30      30
                  22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     286     403     859    1029 
   Size:        286     117     456     170

  Fragments arranged by size: 

                 456     286     170     117
 
BseYI C'CCAG_C

Cuts at:      0     695    1029 
   Size:        695     334
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889    1029 
   Size:        889     140
 
BsiEI CG_ry'CG

Cuts at:      0     135     995    1029 
   Size:        135     860      34

  Fragments arranged by size: 

                 860     135      34
 
BsiHKAI G_wGCw'C

Cuts at:      0     776    1024    1029 
   Size:        776     248       5

  Fragments arranged by size: 

                 776     248       5
 
Bsp1286I G_dGCh'C

Cuts at:      0      63      91     146     574     702     776     893    1024 
   Size:         63      28      55     428     128      74     117     131

Cuts at:   1024    1029 
   Size:          5

  Fragments arranged by size: 

                 428     131     128     117      74      63      55      28
                   5
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     326     482    1029 
   Size:        326     156     547

  Fragments arranged by size: 

                 547     326     156
 
BssHII G'CGCG_C

Cuts at:      0     627    1029 
   Size:        627     402
 
BssSI C'ACGA_G

Cuts at:      0     775    1029 
   Size:        775     254
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     186     327    1029 
   Size:        186     141     702

  Fragments arranged by size: 

                 702     186     141
 
BstYI r'GATC_y

Cuts at:      0     509    1029 
   Size:        509     520
 
Bsu36I CC'TnA_GG

Cuts at:      0     688    1029 
   Size:        688     341
 
BtgI C'CryG_G

Cuts at:      0     135     158     226     235     417    1029 
   Size:        135      23      68       9     182     612

  Fragments arranged by size: 

                 612     182     135      68      23       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     138    1029 
   Size:        138     891
 
BtsI GCAGTG_nn'

Cuts at:      0     497    1029 
   Size:        497     532
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      34    1029 
   Size:         34     995
 
EaeI y'GGCC_r

Cuts at:      0     132     533    1029 
   Size:        132     401     496

  Fragments arranged by size: 

                 496     401     132
 
EagI C'GGCC_G

Cuts at:      0     132    1029 
   Size:        132     897
 
EarI CTCTTCn'nnn_

Cuts at:      0      46     263    1012    1029 
   Size:         46     217     749      17

  Fragments arranged by size: 

                 749     217      46      17
 
EcoICRI GAG'CTC

Cuts at:      0     774    1022    1029 
   Size:        774     248       7

  Fragments arranged by size: 

                 774     248       7
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     586     622     860    1004    1029 
   Size:        355     231      36     238     144      25

  Fragments arranged by size: 

                 355     238     231     144      36      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     466    1029 
   Size:        466     563
 
EcoO109I rG'GnC_Cy

Cuts at:      0      59      60     471     699    1029 
   Size:         59       1     411     228     330

  Fragments arranged by size: 

                 411     330     228      59       1
 
HaeII r_GCGC'y

Cuts at:      0      31     116     235    1029 
   Size:         31      85     119     794

  Fragments arranged by size: 

                 794     119      85      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     187     219     382     398     414     430     463     495 
   Size:        187      32     163      16      16      16      33      32

Cuts at:    495     977    1009    1029 
   Size:        482      32      20

  Fragments arranged by size: 

                 482     187     163      33      32      32      32      20
                  16      16      16
 
KasI G'GCGC_C

Cuts at:      0      27     231    1029 
   Size:         27     204     798

  Fragments arranged by size: 

                 798     204      27
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     425    1029 
   Size:        425     604
 
MslI CAynn'nnrTG

Cuts at:      0     157     577    1029 
   Size:        157     420     452

  Fragments arranged by size: 

                 452     420     157
 
MspA1I CmG'CkG

Cuts at:      0     137     228     419     493     741     823     973    1029 
   Size:        137      91     191      74     248      82     150      56

  Fragments arranged by size: 

                 248     191     150     137      91      82      74      56
            
 
NarI GG'CG_CC

Cuts at:      0      28     232    1029 
   Size:         28     204     797

  Fragments arranged by size: 

                 797     204      28
 
NcoI C'CATG_G

Cuts at:      0     158    1029 
   Size:        158     871
 
PasI CC'CwG_GG

Cuts at:      0     702    1029 
   Size:        702     327
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     768    1029 
   Size:        768     261
 
PfoI T'CCnGG_A

Cuts at:      0     481    1029 
   Size:        481     548
 
PmlI CAC'GTG

Cuts at:      0     575     651     860    1029 
   Size:        575      76     209     169

  Fragments arranged by size: 

                 575     209     169      76
 
PpuMI rG'GwC_Cy

Cuts at:      0     471    1029 
   Size:        471     558
 
PspOMI G'GGCC_C

Cuts at:      0      59     698    1029 
   Size:         59     639     331

  Fragments arranged by size: 

                 639     331      59
 
PstI C_TGCA'G

Cuts at:      0     323     626     795    1029 
   Size:        323     303     169     234

  Fragments arranged by size: 

                 323     303     234     169
 
PvuII CAG'CTG

Cuts at:      0     823     973    1029 
   Size:        823     150      56

  Fragments arranged by size: 

                 823     150      56
 
SacI G_AGCT'C

Cuts at:      0     776    1024    1029 
   Size:        776     248       5

  Fragments arranged by size: 

                 776     248       5
 
SacII CC_GC'GG

Cuts at:      0     138     229     420    1029 
   Size:        138      91     191     609

  Fragments arranged by size: 

                 609     191     138      91
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1012    1029 
   Size:       1012      17
 
SbfI CC_TGCA'GG

Cuts at:      0     795    1029 
   Size:        795     234
 
SfcI C'TryA_G

Cuts at:      0     319     622     791     974    1029 
   Size:        319     303     169     183      55

  Fragments arranged by size: 

                 319     303     183     169      55
 
SfoI GGC'GCC

Cuts at:      0      29     233    1029 
   Size:         29     204     796

  Fragments arranged by size: 

                 796     204      29
 
StuI AGG'CCT

Cuts at:      0     789    1029 
   Size:        789     240
 
StyI C'CwwG_G

Cuts at:      0      10     158     287    1029 
   Size:         10     148     129     742

  Fragments arranged by size: 

                 742     148     129      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      88     228    1029 
   Size:         88     140     801

  Fragments arranged by size: 

                 801     140      88
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     559     591    1029 
   Size:        559      32     438

  Fragments arranged by size: 

                 559     438      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     165     379     936    1029 
   Size:        165     214     557      93

  Fragments arranged by size: 

                 557     214     165      93
 

 Enzymes that do cut:

   AflIII     AhdI     AleI    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI     BglI    BglII Bme1580I    BmgBI     BpmI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspMI   BssHII    BssSI   BstAPI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I     KasI     MmeI     MslI   MspA1I
     NarI     NcoI     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI
     PstI    PvuII     SacI    SacII     SapI     SbfI     SfcI     SfoI
     StuI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaWI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI    BstBI   BstEII    BstXI  BstZ17I     ClaI     DraI   DraIII
     DrdI     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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