Restriction map for pF1KB6518
 (Linear) (Six-base) MAPSORT of: KIBB6518.seq  Check: 8283  from: 1  to: 1158



KIBB6518.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     294     854     957    1158 
   Size:        294     560     103     201

  Fragments arranged by size: 

                 560     294     201     103
 
AccI GT'mk_AC

Cuts at:      0     449    1158 
   Size:        449     709
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     196     817    1158 
   Size:        114      82     621     341

  Fragments arranged by size: 

                 621     341     114      82
 
AleI CACnn'nnGTG

Cuts at:      0     103    1158 
   Size:        103    1055
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     883    1158 
   Size:        883     275
 
AlwNI CAG_nnn'CTG

Cuts at:      0     137     375     504    1158 
   Size:        137     238     129     654

  Fragments arranged by size: 

                 654     238     137     129
 
ApaI G_GGCC'C

Cuts at:      0      93    1158 
   Size:         93    1065
 
AvaI C'yCGr_G

Cuts at:      0      21    1158 
   Size:         21    1137
 
BanI G'GyrC_C

Cuts at:      0     939    1158 
   Size:        939     219
 
BanII G_rGCy'C

Cuts at:      0      14      93     646    1158 
   Size:         14      79     553     512

  Fragments arranged by size: 

                 553     512      79      14
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1036    1051    1158 
   Size:       1036      15     107

  Fragments arranged by size: 

                1036     107      15
 
Bme1580I G_kGCm'C

Cuts at:      0      83      93     944    1158 
   Size:         83      10     851     214

  Fragments arranged by size: 

                 851     214      83      10
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      70    1077    1101    1158 
   Size:         70    1007      24      57

  Fragments arranged by size: 

                1007      70      57      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     295     802    1158 
   Size:         39     256     507     356

  Fragments arranged by size: 

                 507     356     256      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     117     475     517    1158 
   Size:        117     358      42     641

  Fragments arranged by size: 

                 641     358     117      42
 
BsaAI yAC'GTr

Cuts at:      0     549    1158 
   Size:        549     609
 
BsaHI Gr'CG_yC

Cuts at:      0     614    1158 
   Size:        614     544
 
BsaWI w'CCGG_w

Cuts at:      0     152    1158 
   Size:        152    1006
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     283     417     718    1158 
   Size:        112     171     134     301     440

  Fragments arranged by size: 

                 440     301     171     134     112
 
BseYI C'CCAG_C

Cuts at:      0     228     555     999    1158 
   Size:        228     327     444     159

  Fragments arranged by size: 

                 444     327     228     159
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     688     985    1158 
   Size:        274     414     297     173

  Fragments arranged by size: 

                 414     297     274     173
 
BsiEI CG_ry'CG

Cuts at:      0     597     758    1043    1158 
   Size:        597     161     285     115

  Fragments arranged by size: 

                 597     285     161     115
 
BsiHKAI G_wGCw'C

Cuts at:      0     646    1158 
   Size:        646     512
 
BsmI GAATG_Cn'

Cuts at:      0     383    1158 
   Size:        383     775
 
Bsp1286I G_dGCh'C

Cuts at:      0      14      83      93     646     944    1158 
   Size:         14      69      10     553     298     214

  Fragments arranged by size: 

                 553     298     214      69      14      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     294     461     854     957    1158 
   Size:        294     167     393     103     201

  Fragments arranged by size: 

                 393     294     201     167     103
 
BsrDI GCAATG_nn'

Cuts at:      0     401     615    1100    1158 
   Size:        401     214     485      58

  Fragments arranged by size: 

                 485     401     214      58
 
BsrFI r'CCGG_y

Cuts at:      0    1045    1158 
   Size:       1045     113
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1119    1158 
   Size:       1119      39
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     108    1158 
   Size:        108    1050
 
BstYI r'GATC_y

Cuts at:      0     414     825    1158 
   Size:        414     411     333

  Fragments arranged by size: 

                 414     411     333
 
BtgI C'CryG_G

Cuts at:      0      69     647     697     752    1031    1090    1158 
   Size:         69     578      50      55     279      59      68

  Fragments arranged by size: 

                 578     279      69      68      59      55      50
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      17    1158 
   Size:         17    1141
 
EaeI y'GGCC_r

Cuts at:      0      95     366     661     755    1028    1040    1082    1158 
   Size:         95     271     295      94     273      12      42      76

  Fragments arranged by size: 

                 295     273     271      95      94      76      42      12
            
 
EagI C'GGCC_G

Cuts at:      0     755    1040    1158 
   Size:        755     285     118

  Fragments arranged by size: 

                 755     285     118
 
EarI CTCTTCn'nnn_

Cuts at:      0     151     178    1158 
   Size:        151      27     980

  Fragments arranged by size: 

                 980     151      27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     186    1087    1158 
   Size:        186     901      71

  Fragments arranged by size: 

                 901     186      71
 
EcoICRI GAG'CTC

Cuts at:      0     644    1158 
   Size:        644     514
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     114     196     295     802     817    1158 
   Size:         39      75      82      99     507      15     341

  Fragments arranged by size: 

                 507     341      99      82      75      39      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     850    1158 
   Size:        850     308
 
EcoO109I rG'GnC_Cy

Cuts at:      0      25      90     540     633    1158 
   Size:         25      65     450      93     525

  Fragments arranged by size: 

                 525     450      93      65      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     586     618    1158 
   Size:        586      32     540

  Fragments arranged by size: 

                 586     540      32
 
HindIII A'AGCT_T

Cuts at:      0     715     923    1158 
   Size:        715     208     235

  Fragments arranged by size: 

                 715     235     208
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     145    1158 
   Size:        145    1013
 
MscI TGG'CCA

Cuts at:      0      97     368    1084    1158 
   Size:         97     271     716      74

  Fragments arranged by size: 

                 716     271      97      74
 
MslI CAynn'nnrTG

Cuts at:      0     103     580    1158 
   Size:        103     477     578

  Fragments arranged by size: 

                 578     477     103
 
MspA1I CmG'CkG

Cuts at:      0     134     372     699    1092    1158 
   Size:        134     238     327     393      66

  Fragments arranged by size: 

                 393     327     238     134      66
 
NaeI GCC'GGC

Cuts at:      0    1047    1158 
   Size:       1047     111
 
NgoMIV G'CCGG_C

Cuts at:      0    1045    1158 
   Size:       1045     113
 
NotI GC'GGCC_GC

Cuts at:      0    1040    1158 
   Size:       1040     118
 
NspI r_CATG'y

Cuts at:      0    1080    1158 
   Size:       1080      78
 
PasI CC'CwG_GG

Cuts at:      0      86    1158 
   Size:         86    1072
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     375    1158 
   Size:        375     783
 
PfoI T'CCnGG_A

Cuts at:      0       5     779    1158 
   Size:          5     774     379

  Fragments arranged by size: 

                 774     379       5
 
PpuMI rG'GwC_Cy

Cuts at:      0     540     633    1158 
   Size:        540      93     525

  Fragments arranged by size: 

                 540     525      93
 
PshAI GACnn'nnGTC

Cuts at:      0      56    1158 
   Size:         56    1102
 
PspOMI G'GGCC_C

Cuts at:      0      89    1158 
   Size:         89    1069
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     898     930    1158 
   Size:        898      32     228

  Fragments arranged by size: 

                 898     228      32
 
PstI C_TGCA'G

Cuts at:      0     338     689     854    1158 
   Size:        338     351     165     304

  Fragments arranged by size: 

                 351     338     304     165
 
PvuII CAG'CTG

Cuts at:      0     134     372    1158 
   Size:        134     238     786

  Fragments arranged by size: 

                 786     238     134
 
RsrII CG'GwC_CG

Cuts at:      0    1131    1158 
   Size:       1131      27
 
SacI G_AGCT'C

Cuts at:      0     646    1158 
   Size:        646     512
 
SacII CC_GC'GG

Cuts at:      0     700    1093    1158 
   Size:        700     393      65

  Fragments arranged by size: 

                 700     393      65
 
SanDI GG'GwC_CC

Cuts at:      0     633    1158 
   Size:        633     525
 
SapI GCTCTTCn'nnn_

Cuts at:      0     151     178    1158 
   Size:        151      27     980

  Fragments arranged by size: 

                 980     151      27
 
ScaI AGT'ACT

Cuts at:      0     340    1158 
   Size:        340     818
 
SfcI C'TryA_G

Cuts at:      0     334     685     850    1158 
   Size:        334     351     165     308

  Fragments arranged by size: 

                 351     334     308     165
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1045    1158 
   Size:       1045     113
 
SmaI CCC'GGG

Cuts at:      0      23    1158 
   Size:         23    1135
 
StuI AGG'CCT

Cuts at:      0     119     673    1158 
   Size:        119     554     485

  Fragments arranged by size: 

                 554     485     119
 
StyI C'CwwG_G

Cuts at:      0     410     485    1158 
   Size:        410      75     673

  Fragments arranged by size: 

                 673     410      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1148    1158 
   Size:       1148      10
 
TatI w'GTAC_w

Cuts at:      0     338    1158 
   Size:        338     820
 
Tth111I GACn'n_nGTC

Cuts at:      0     952    1158 
   Size:        952     206
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      83     105     671    1023    1158 
   Size:         83      22     566     352     135

  Fragments arranged by size: 

                 566     352     135      83      22
 
XmaI C'CCGG_G

Cuts at:      0      21    1158 
   Size:         21    1137
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AlfI    AlwNI     ApaI     AvaI
     BanI    BanII     BglI Bme1580I     BmrI     BpmI     BsaI    BsaAI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI
    BtgZI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     NotI     NspI     PasI    PflMI     PfoI    PpuMI    PshAI
   PspOMI     PsrI     PstI    PvuII    RsrII     SacI    SacII    SanDI
     SapI     ScaI     SfcI    SgrAI     SmaI     StuI     StyI    TaqII
     TatI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaBI
    BsaXI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NcoI     NdeI     NheI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI     PsiI    PspXI     PvuI     SalI     SbfI
    SexAI     SfiI     SfoI     SgfI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI     XbaI     XhoI     XmnI     ZraI
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