Restriction map for pF1KB6504
 (Linear) (Six-base) MAPSORT of: KIBB6504.seq  Check: 2928  from: 1  to: 1146



KIBB6504.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

Acc65I G'GTAC_C

Cuts at:      0    1005    1146 
   Size:       1005     141
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     703    1146 
   Size:        703     443
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     307    1073    1146 
   Size:        307     766      73

  Fragments arranged by size: 

                 766     307      73
 
AvaI C'yCGr_G

Cuts at:      0     318     327     949    1146 
   Size:        318       9     622     197

  Fragments arranged by size: 

                 622     318     197       9
 
BamHI G'GATC_C

Cuts at:      0     264     402     773    1146 
   Size:        264     138     371     373

  Fragments arranged by size: 

                 373     371     264     138
 
BanI G'GyrC_C

Cuts at:      0     164     722    1005    1146 
   Size:        164     558     283     141

  Fragments arranged by size: 

                 558     283     164     141
 
BanII G_rGCy'C

Cuts at:      0     136     791     819     959    1145    1146 
   Size:        136     655      28     140     186       1

  Fragments arranged by size: 

                 655     186     140     136      28       1
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     980    1146 
   Size:        980     166
 
BbvCI CC'TCA_GC

Cuts at:      0     874    1146 
   Size:        874     272
 
BlpI GC'TnA_GC

Cuts at:      0     613    1146 
   Size:        613     533
 
Bme1580I G_kGCm'C

Cuts at:      0     569     576    1146 
   Size:        569       7     570

  Fragments arranged by size: 

                 570     569       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     376     604     628    1146 
   Size:        300      76     228      24     518

  Fragments arranged by size: 

                 518     300     228      76      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     874    1146 
   Size:        874     272
 
BsaAI yAC'GTr

Cuts at:      0     393    1146 
   Size:        393     753
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     998    1028    1146 
   Size:        998      30     118

  Fragments arranged by size: 

                 998     118      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     193     891    1146 
   Size:        193     698     255

  Fragments arranged by size: 

                 698     255     193
 
BseYI C'CCAG_C

Cuts at:      0      36     189     731    1146 
   Size:         36     153     542     415

  Fragments arranged by size: 

                 542     415     153      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     880    1146 
   Size:        880     266
 
BsiHKAI G_wGCw'C

Cuts at:      0     286     791    1016    1146 
   Size:        286     505     225     130

  Fragments arranged by size: 

                 505     286     225     130
 
BsmI GAATG_Cn'

Cuts at:      0    1109    1146 
   Size:       1109      37
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     280    1146 
   Size:        280     866
 
Bsp1286I G_dGCh'C

Cuts at:      0     136     286     569     576     791     819     959    1016 
   Size:        136     150     283       7     215      28     140      57

Cuts at:   1016    1145    1146 
   Size:        129       1

  Fragments arranged by size: 

                 283     215     150     140     136     129      57      28
                   7       1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     373     917    1146 
   Size:        373     544     229

  Fragments arranged by size: 

                 544     373     229
 
BsrBI CCG'CTC

Cuts at:      0     158    1146 
   Size:        158     988
 
BsrDI GCAATG_nn'

Cuts at:      0     453    1146 
   Size:        453     693
 
BstEII G'GTnAC_C

Cuts at:      0     642    1146 
   Size:        642     504
 
BstYI r'GATC_y

Cuts at:      0     264     402     773    1146 
   Size:        264     138     371     373

  Fragments arranged by size: 

                 373     371     264     138
 
BtgI C'CryG_G

Cuts at:      0      15      91     128     551    1088    1146 
   Size:         15      76      37     423     537      58

  Fragments arranged by size: 

                 537     423      76      58      37      15
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1069    1146 
   Size:       1069      77
 
EaeI y'GGCC_r

Cuts at:      0     125     455    1146 
   Size:        125     330     691

  Fragments arranged by size: 

                 691     330     125
 
EarI CTCTTCn'nnn_

Cuts at:      0     523     664     668    1146 
   Size:        523     141       4     478

  Fragments arranged by size: 

                 523     478     141       4
 
EcoICRI GAG'CTC

Cuts at:      0     789    1146 
   Size:        789     357
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     376     604     628     703    1146 
   Size:        300      76     228      24      75     443

  Fragments arranged by size: 

                 443     300     228      76      75      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     207     694    1146 
   Size:        207     487     452

  Fragments arranged by size: 

                 487     452     207
 
HaeII r_GCGC'y

Cuts at:      0      81     124     682    1146 
   Size:         81      43     558     464

  Fragments arranged by size: 

                 558     464      81      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     273     305    1146 
   Size:        273      32     841

  Fragments arranged by size: 

                 841     273      32
 
KpnI G_GTAC'C

Cuts at:      0    1009    1146 
   Size:       1009     137
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     969    1146 
   Size:        969     177
 
MscI TGG'CCA

Cuts at:      0     457    1146 
   Size:        457     689
 
MspA1I CmG'CkG

Cuts at:      0      17      93     189     860    1146 
   Size:         17      76      96     671     286

  Fragments arranged by size: 

                 671     286      96      76      17
 
PasI CC'CwG_GG

Cuts at:      0     426    1146 
   Size:        426     720
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     738    1146 
   Size:        738     408
 
PfoI T'CCnGG_A

Cuts at:      0     353    1146 
   Size:        353     793
 
PmlI CAC'GTG

Cuts at:      0     393    1146 
   Size:        393     753
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      35      67    1146 
   Size:         35      32    1079

  Fragments arranged by size: 

                1079      35      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     207     694    1146 
   Size:        207     487     452

  Fragments arranged by size: 

                 487     452     207
 
PstI C_TGCA'G

Cuts at:      0     917    1146 
   Size:        917     229
 
RsrII CG'GwC_CG

Cuts at:      0     855    1146 
   Size:        855     291
 
SacI G_AGCT'C

Cuts at:      0     791    1146 
   Size:        791     355
 
SacII CC_GC'GG

Cuts at:      0      18      94    1146 
   Size:         18      76    1052

  Fragments arranged by size: 

                1052      76      18
 
SanDI GG'GwC_CC

Cuts at:      0     207     694    1146 
   Size:        207     487     452

  Fragments arranged by size: 

                 487     452     207
 
SapI GCTCTTCn'nnn_

Cuts at:      0     523     664    1146 
   Size:        523     141     482

  Fragments arranged by size: 

                 523     482     141
 
SfcI C'TryA_G

Cuts at:      0     834     913    1146 
   Size:        834      79     233

  Fragments arranged by size: 

                 834     233      79
 
SmaI CCC'GGG

Cuts at:      0     320     329     951    1146 
   Size:        320       9     622     195

  Fragments arranged by size: 

                 622     320     195       9
 
StyI C'CwwG_G

Cuts at:      0     469     698    1146 
   Size:        469     229     448

  Fragments arranged by size: 

                 469     448     229
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1097    1101    1146 
   Size:       1097       4      45

  Fragments arranged by size: 

                1097      45       4
 
TatI w'GTAC_w

Cuts at:      0     938    1146 
   Size:        938     208
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     275    1146 
   Size:        275     871
 
XmaI C'CCGG_G

Cuts at:      0     318     327     949    1146 
   Size:        318       9     622     197

  Fragments arranged by size: 

                 622     318     197       9
 
XmnI GAAnn'nnTTC

Cuts at:      0     517    1146 
   Size:        517     629
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BlpI Bme1580I     BpmI   Bpu10I    BsaAI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI   BstEII    BstYI     BtgI     DrdI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I     KpnI     MmeI     MscI   MspA1I
     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI     PstI    RsrII
     SacI    SacII    SanDI     SapI     SfcI     SmaI     StyI    TaqII
     TatI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII     SalI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com